LOCUS       sequence-198434-       12102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALE3 under the control of a 0.8kb ase enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA
  TITLE     A multiplexable TALE-based binary expression system for in vivo 
            cellular interaction studies.
  JOURNAL   Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3.
  PUBMED    29162808
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-21"; volume: "8(1):1663. doi"; pages: " 
            10.1038/s41467-017-01592-3"
COMMENT     Sequence Label: pJC-ase0.8kb-TALE3-VP64-P10
FEATURES             Location/Qualifiers
     source          1..12102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7507..7528
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7560..7626
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             7664..7684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7685..7711
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             11204..11353
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(12048..12066)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttccaatgc aagtcctggt aaatatatgt atatattatc cagtacgagt
     6541 ttttgaaaag ttaacaatag gtgatcccga gacatttttc gaatgaagta gaaaccagtc
     6601 cttggtttta gctataagct aaaaataaag attcgatgca tttctgcgtt ttacatgacg
     6661 aatattggag gtctaaggtg atctattagg atattttgca aattcctagg tggtagggca
     6721 ttccttgaaa accagggctg aaaaagctcc ccagggaata tactttttat tatatgcata
     6781 cgtatatggt tattataatg tccatatatt aataggggcg gtatataagc ataatgtgtt
     6841 tgctgccgat aaataatgag agagagcgtg gcgaaatcca cccttgaacc aggtggactt
     6901 tttggctcga gttgatcaga tgttagtttt cccaaaagcc gtactgtata taatatatat
     6961 atatgataaa ggtgtatgtg tgagcgatcg agaagggaca cccagtctag gggatgaaaa
     7021 gtcaggccct ttacataagg gatacgcagg acctcaaatg ccttctgttt tgtatgtgtg
     7081 tgctctgtgt tgtgtatgtc agtcaacgaa gtcacttccg ttgggtttgc gttttagttt
     7141 gagttcggag tttaggggca cgcgacacag agcgccagca gctgtcctga tgcaaggaca
     7201 cggaaaccat attacatcag tcaccagtta acattcactc aagaaggact aacttgctaa
     7261 aagtacaccc gcaatcgcca ccagtttttc tcccgccctc aaaacatagg ccggccatac
     7321 gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa
     7381 gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac
     7441 aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa
     7501 tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagag atctaaaagg
     7561 taggttcaac cactgatgcc taggcacacc gaaacgacta accctaattc ttatccttta
     7621 cttcaggcgg ccgcggctcg agctcgagat ggcttcctcc cctccaaaga aaaagagaaa
     7681 ggttagttgg aaggacgcaa gtggttggtc tagaatgcat gcggatccca ttcgtccgcg
     7741 caggccaagt cctgcccgcg agcttctgcc cggaccccaa ccggataggg ttcagccgac
     7801 tgcagatcgt ggggtgtctg cgcctgctgg cagccctctg gatggcttgc ccgctcggcg
     7861 gacggtgtcc cggacccggc tgccatctcc ccctgcgccc tcacctgcgt tctcggcggg
     7921 cagcttcagc gatctgctcc gtccgttcga tccgtcgctt cttgatacat cgcttcttga
     7981 ttcgatgcct gccgtcggca cgccgcatac agcggctgcc ccagcagagt gggatgaggc
     8041 gcaatcggct ctgcgtgcag ccgatgaccc gccacccacc gtgcgtgtcg ctgtcactgc
     8101 cgcgcggccg ccgcgcgcca agccggcccc gcgacggcgt gctgcgcaac cctccgacgc
     8161 ttcgccggcc gcgcaggtgg atctacgcac gctcggctac agtcagcagc agcaagagaa
     8221 gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg tgggccatgg
     8281 gtttacacac gcgcacatcg ttgcgctcag ccaacacccg gcagcgttag ggaccgtcgc
     8341 tgtcacgtat cagcacataa tcacggcgtt gccagaggcg acacacgaag acatcgttgg
     8401 cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc ttgctcacgg atgcggggga
     8461 gttgagaggt ccgccgttac agttggacac aggccaactt gtgaagattg caaaacgtgg
     8521 cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat gcactgacgg gtgcccccct
     8581 gaacctgacc ccggaccaag tggtggctat cgccagcaac attggcggca agcaagcgct
     8641 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ctccggacca
     8701 agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     8761 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
     8821 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     8881 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacaatg gcggcaagca
     8941 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     9001 ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca
     9061 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     9121 cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     9181 gtgccaggac catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg
     9241 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     9301 gaccccggac caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac
     9361 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     9421 ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     9481 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
     9541 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     9601 tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca agcaagcgct
     9661 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     9721 agtggtggct atcgccagca acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     9781 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
     9841 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     9901 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacaatg gcggcaagca
     9961 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc
    10021 ggaccaagtg gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca
    10081 gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag tggtggctat
    10141 cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
    10201 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg
    10261 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
    10321 gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac
    10381 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
    10441 ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa agcattgtgg cccagctgag
    10501 ccggcctgat ccggcgttgg ccgcgttgac caacgaccac ctcgtcgcct tggcctgcct
    10561 cggcggacgt cctgccatgg atgcagtgaa aaagggattg ccgcacgcgc cggaattgat
    10621 cagaagagtc aatcgccgta ttggcgaacg cacgtcccat cgcgttgccg actacgcgca
    10681 agtggttcgc gtgctggagt ttttccagtg ccactcccac ccagcgtacg catttgatga
    10741 ggccatgacg cagttcggga tgagcaggaa cgggttggta cagctctttc gcagagtggg
    10801 cgtcaccgaa ctcgaagccc gcggtggaac gctcccccca gcctcgcagc gttgggaccg
    10861 tatcctccag gcatcaggga tgaaaagggc caaaccgtcc cctacttcag ctcaaacacc
    10921 ggatcaggcg tctttgcatg cattcgccga ttcgctggag cgtgaccttg atgcgcccag
    10981 cccaatgcac gagggagatc agacgcgggc aagcagccgt aaacggtccc gatcggatcg
    11041 tgctgtcacc ggcccctccg cacagcaggc tgtcgaggtg cgcgttcccg aacagcgcga
    11101 tgcgctgcat ttgcccctca gctggagggt aaaacgcccg cgtaccagga tctggggcgg
    11161 cctcccggat ccgatatcta gatcccaggg ttccggacgg gctgacgcat tggacgattt
    11221 tgatctggat atgctgggaa gtgacgccct cgatgatttt gaccttgaca tgcttggttc
    11281 ggatgccctt gatgactttg acctcgacat gctcggcagt gacgcccttg atgatttcga
    11341 cctggacatg ctgatctaga aatgaatcgt ttttaaaata acaaatcaat tgttttataa
    11401 tattcgtacg attctttgat tatgtaataa aatgtgatca ttaggaagat tacgaaaaat
    11461 ataaaaaata tgagttctgt gtgtataaca aatgctgtaa acgccacaat tgtgtttgtt
    11521 gcaaataaac ccatgattat ttgattaaaa ttgttgtttt ctttgttcat agacaatagt
    11581 gtgttttgcc taaacgtgta ctgcataaac tccatgcgag tgtatagcga gctagtggct
    11641 aacgcttgcc ccaccaaagt agattcgtca aaatcctcaa tttcatcacc ctcctccaag
    11701 tttaacattt ggccgtcgga attaacttct aaagatgcca cataatctaa taaatgaaat
    11761 agagattcaa acgtggcgtc atcgtccgtt tcgaccattt ccgaaaagaa ctcgggcata
    11821 aactctatga tttctctgga cgtggtgttg tcgaaactct caaagtacgc agtcaggaac
    11881 gtgcgcgaca tgtcgtcggg aaactcgcgc ggaaacatgt tgttgtaacc gaacgggtcc
    11941 catagcgcca aaaccaaatc tgccagcgtc aatagaatga gcacgatgcc gacaatggag
    12001 ctggcttgga tagcgattcg agttaacgaa ttcttgaaga cgaaagggcc tcgtgatacg
    12061 cctattttta taggttaatg tcatgataat aatggtttct ta
//
