LOCUS       sequence-198455-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression of AsCpf1-2NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, 
            Khokha MK, Doudna JA, Giraldez AJ
  TITLE     CRISPR-Cpf1 mediates efficient homology-directed repair and 
            temperature-controlled genome editing.
  JOURNAL   Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
  PUBMED    29222508
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-12-8"; volume: "8(1):2024. doi"; pages: " 
            10.1038/s41467-017-01836-2"
COMMENT     Sequence Label: AsCpf1-2NLS
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    144..283
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(209..228)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     369..391
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(385..576)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2034..2053
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2137..2156
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2137..2155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2244..2266
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2286..2303
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2313..3413
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     3387..3410
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3468..3488
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3498..7418
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     CDS             7422..7448
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7428..7454
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7467..7487
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7494..7514
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7554..8258
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7696..7714)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7964..7983
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8265..8285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8417..8435)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8431..8478
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8844..8849,8850..8866,8867..8872))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8899..8917)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8985..9089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9090..9158,9159..9950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9308..9327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10121..10709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10610..10629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
       61 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      121 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      181 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
      241 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
      301 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      361 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      421 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
      481 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
      541 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
      601 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
      661 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
      721 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt
      781 aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca
      841 gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt
      901 tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg
      961 tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag
     1021 gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg
     1081 gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac
     1141 agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg
     1201 ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg
     1261 aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc
     1321 gaggcggcat aaatcgccgt gacgatcagc ggtccaatga tcgaagttag gctggtaaga
     1381 gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
     1441 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
     1501 atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg
     1561 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga
     1621 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag
     1681 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc
     1741 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag
     1801 gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc
     1861 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     1921 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     1981 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     2041 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     2101 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac
     2161 cacaacggtt tccctctagt gccggctccg gagagctctt taattaagcg gccgccctgc
     2221 aggactcgag ttctagaaat aattttgttt aactttaaga aggagatata catatgaaat
     2281 cttctcacca tcaccatcac catggttctt ctatgaaaat cgaagaaggt aaactggtaa
     2341 tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga
     2401 aagataccgg aattaaagtc accgttgagc atccggataa actggaagag aaattcccac
     2461 aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg
     2521 gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc
     2581 tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg
     2641 ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct
     2701 gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt
     2761 tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt
     2821 tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga
     2881 aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg
     2941 attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc
     3001 cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga
     3061 ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg
     3121 ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag
     3181 gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg
     3241 aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag aaaggtgaaa
     3301 tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca
     3361 acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga
     3421 gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggaa aacctgtact
     3481 tccaatccaa tgccaccatg acccagttcg aggggtttac caatctgtat caagtgagca
     3541 agacgctgcg ctttgaactg atcccacagg gaaaaacctt aaaacatatt caagagcagg
     3601 gctttatcga agaagataag gcccgtaatg accattacaa agagttaaag ccgattattg
     3661 atcgtatcta caagacctat gcggaccagt gcttacaatt ggtacagctt gattgggaga
     3721 acctctctgc cgccatcgat tcctatcgta aagaaaaaac tgaagaaacg cgcaacgccc
     3781 tgattgaaga gcaggccacc tatcgtaacg cgattcatga ctattttatt ggccgtacgg
     3841 acaatctgac ggacgcgatc aacaagcgcc atgcggagat ttacaaagga ctgtttaagg
     3901 ctgaactgtt caatggtaag gtccttaaac agcttgggac cgtcacaacg acggaacatg
     3961 aaaacgcgtt attacgtagc ttcgacaagt ttaccacgta tttctccggc ttttacgaaa
     4021 atcgcaaaaa cgttttcagt gccgaggata tttccactgc tatccctcat cgcattgtgc
     4081 aagacaactt cccaaaattc aaagaaaatt gtcatatctt cacccgctta atcaccgctg
     4141 taccgtccct gcgtgagcat ttcgaaaacg tgaaaaaggc cattggtatc ttcgtgtcta
     4201 cttcgattga ggaggtattt tcctttccat tctataatca gctgctgacc cagacccaaa
     4261 ttgatctgta caaccagctg cttggcggta tttctcgtga agcaggaacc gaaaaaatca
     4321 aagggttgaa cgaggtgctt aatctggcaa tccagaaaaa tgatgaaacc gcccacatca
     4381 ttgcttcgtt acctcatcgt tttatcccgt tgttcaagca aattttaagt gatcgcaata
     4441 cgctgtcgtt tattctggaa gaattcaaaa gtgatgaaga ggtaattcag tcgttttgca
     4501 aatataaaac cctgttacgt aacgaaaatg tcctggaaac agccgaggct ttgtttaacg
     4561 aactgaatag cattgacctg acgcatatct ttattagcca caaaaaatta gagaccatct
     4621 catcagctct gtgcgatcat tgggatacac tgcgcaatgc gctgtatgaa cgtcgtattt
     4681 cggaattgac tggcaaaatc actaaaagcg cgaaagagaa agtacagcgc tcgcttaaac
     4741 atgaagatat caacctgcag gagatcatca gcgccgcggg taaagaactg tcggaggcat
     4801 ttaaacagaa gacgagcgag attctgtccc acgcacatgc cgccttagac cagccgctcc
     4861 cgaccactct gaagaaacag gaagagaaag aaatccttaa aagtcaactg gacagtttac
     4921 tgggtctcta tcatctgctg gattggtttg cggtagacga aagcaatgaa gtggatccgg
     4981 agtttagtgc ccgtctgaca ggaatcaagc tggaaatgga gccttcgctt agcttctaca
     5041 acaaagcccg caattatgcc acgaaaaaac cctatagtgt cgaaaaattt aaactcaact
     5101 ttcaaatgcc gacccttgcg tcgggctggg atgtcaacaa agaaaaaaac aacggagcta
     5161 ttctgttcgt taaaaatggt ctgtactacc tgggcatcat gccgaaacag aaaggtcgct
     5221 acaaagccct ttcgttcgag cccacggaaa aaacaagcga aggcttcgac aaaatgtact
     5281 acgattactt tccggatgca gcaaaaatga tcccgaaatg ttccacacag ctgaaagccg
     5341 ttacagcaca ttttcagacg cacaccaccc ccatcttact gtccaacaat tttattgaac
     5401 cgctggagat tactaaagaa atttatgatt tgaacaatcc ggaaaaagag ccaaaaaagt
     5461 ttcaaaccgc ctacgctaaa aaaaccgggg atcagaaagg gtaccgcgaa gcgttgtgca
     5521 agtggattga tttcacccgc gattttctca gtaaatatac caagactacc tcgattgacc
     5581 tgagctcact gcgcccgagc tctcaatata aggatttggg tgagtactat gctgaattaa
     5641 accctttatt gtaccacatt tcttttcagc gcatcgccga aaaggaaatt atggacgcag
     5701 tcgaaaccgg gaaactgtac ctgttccaga tctataataa ggacttcgcc aaaggacatc
     5761 atggcaaacc gaacctgcac accctttact ggaccgggct tttctctccg gaaaatttgg
     5821 cgaaaacctc gatcaagctt aacggtcaag ctgagctgtt ttaccgtcca aaatcccgca
     5881 tgaagcgcat ggcgcatcgt ttaggtgaaa aaatgctgaa taagaaactg aaagatcaga
     5941 aaacccctat cccggatacc ctctaccagg aactgtatga ttacgtgaac catcgtctct
     6001 cgcatgacct gtcagacgaa gcgcgtgcgt tactgcccaa tgtaatcaca aaagaagttt
     6061 cgcatgaaat tattaaagat cgtcgtttta catctgataa attctttttt catgttccga
     6121 tcaccctcaa ctatcaggcc gcaaacagtc caagtaagtt taaccagcgc gttaatgctt
     6181 acctgaagga acatccggag actccgatta ttggaattga tcgcggtgaa cgtaatttga
     6241 tctatatcac tgtgatcgat agtaccggta agattctgga gcagcgcagc ttgaacacaa
     6301 ttcaacagtt tgattatcag aaaaaattag acaaccgcga aaaagagcgc gtggctgccc
     6361 gtcaggcgtg gtctgttgtc ggtaccatta aagatctgaa gcagggctat ctttctcagg
     6421 ttattcacga aattgtagat ctgatgatcc attatcaggc ggttgttgtg ttggagaatc
     6481 tcaatttcgg ttttaagagt aagcgcacag gcatcgctga aaaagcagtt tatcagcagt
     6541 ttgaaaaaat gctgatcgac aaattgaact gtttagttct caaagattac ccagcggaaa
     6601 aggtgggcgg agtgctgaat ccgtaccaat taacggatca attcacttcc ttcgcaaaga
     6661 tgggtaccca aagcggcttt ctgttctatg tgccggcccc gtatacctcg aaaatcgatc
     6721 cactgacggg cttcgtagat ccgttcgtgt ggaaaaccat taaaaatcat gaaagtcgta
     6781 aacattttct cgaaggcttc gacttcctgc actacgacgt gaaaactggc gatttcattc
     6841 tgcattttaa aatgaaccgc aacctttcgt ttcagcgcgg tctgccgggc tttatgccgg
     6901 cttgggacat tgtttttgag aaaaatgaaa cccagtttga tgctaaaggc actcctttca
     6961 tcgccggtaa acgcatcgta cctgtgattg aaaaccatcg ttttacaggg cgttaccgtg
     7021 atttataccc ggcgaacgaa ttgatcgcgc tgctggagga aaagggcatc gttttccgtg
     7081 acggctccaa tattctgccg aaattactgg aaaacgacga ttcacacgca attgatacca
     7141 tggtcgcact gattcgctca gtcttacaga tgcgtaactc taatgcagcc acaggagaag
     7201 attatattaa ttcgccagtc cgcgatttga acggtgtttg cttcgacagc cgttttcaga
     7261 atcctgaatg gccgatggac gctgatgcca acggagctta tcatatcgcc ctgaaaggcc
     7321 agctcctgct gaaccacctg aaggaaagca aagatctgaa attgcagaac ggcattagca
     7381 accaggactg gttagcatac atccaggaac tgcgtaacgc ctatccctat gacgtgcccg
     7441 attatgccag cctgggcagc ggctccccca agaaaaaacg caaggtggaa gatcctaaga
     7501 aaaagcggaa agtggactaa attggtagtg ggagcaacgg cagcagcgga tccgtgagca
     7561 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     7621 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     7681 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     7741 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     7801 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     7861 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctccaggac ggcgagttca
     7921 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     7981 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     8041 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     8101 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     8161 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     8221 gccgccactc caccggcggc atggacgagc tgtacaaggg gtcacctaag aaaaagcgaa
     8281 aagttgagta ataacattgg aagtggataa cggatccgcg atcgcggcgc gccacctggt
     8341 ggccggccgg taccacgcgt gcgcgctgat ccggctgcta acaaagcccg aaaggaagct
     8401 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     8461 gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt
     8521 ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg
     8581 gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc
     8641 atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg
     8701 caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt
     8761 gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca
     8821 atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga
     8881 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     8941 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9001 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9061 tgcttcaata acattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9121 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9181 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9241 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9301 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     9361 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9421 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9481 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9541 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9601 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     9661 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9721 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9781 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9841 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9901 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9961 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10021 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10141 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10261 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10441 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10501 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10561 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10621 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10681 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10741 tggccttttg c
//
