LOCUS       sequence-198459-        4004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ShadowY in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Murakoshi H, Shibata ACE
  TITLE     ShadowY: a dark yellow fluorescent protein for FLIM-based FRET 
            measurement.
  JOURNAL   Sci Rep. 2017 Jul 28;7(1):6791. doi: 10.1038/s41598-017-07002-4.
  PUBMED    28754922
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-07-28"; volume: "7(1):6791. doi"; pages: " 
            10.1038/s41598-017-07002-4"
COMMENT     Sequence Label: CMV-ShadowY
FEATURES             Location/Qualifiers
     source          1..4004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        112..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     570..590
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      658..667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(709..730)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(970..989)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1317..1338
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1381..1446
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1571..1692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1608..1627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1662..1681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1699..2154
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1786..1805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1996..2017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2210..2314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2315..2383,2384..3175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2533..2552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3346..3934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3835..3854
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttccctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc
      661 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      721 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      781 tacggcaagc tgaccctgaa gctcatctgc accaccggca agctgcccgt gccctggccc
      841 accctcgtga ccaccttcgg ctacggcctg atgtgcttcg cgcgctatcc agaccacatg
      901 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      961 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1021 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1081 cacaagctcg agtacgcttg gcccgtggtg aatgtctata tcatggccga caagcagaag
     1141 aacggcatca aggtgaactt ctctatctat cataacatcg aggacggcag cgtgcagctc
     1201 gccgaccact accagcagaa cacccccatc ggcgacgggc ccgtgctgct gcccgacaac
     1261 cactacctga gctactcggc taagctgagc aaagacccca acgagaagcg cgatcacatg
     1321 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1381 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
     1441 ggatcctaag cggccgcgac actagatcat aatcagccat accacatttg tagaggtttt
     1501 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     1561 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1621 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1681 caatgtatct taacgcgtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1741 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     1801 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     1861 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     1921 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     1981 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2041 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2101 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctcta
     2161 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2221 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2281 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2341 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2401 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2461 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2521 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2581 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2641 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2701 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2761 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2821 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2881 caaactatta actggcgaac tacttactct agcttcccgg caacaactga tagactggat
     2941 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3001 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3061 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3121 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3181 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3241 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3301 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3361 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3421 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3481 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3541 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3601 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3661 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3721 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3781 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3841 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3901 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3961 gttcctggcc ttttgctggc cttttgctca catggctcga caga
//
