LOCUS       sequence-198461-        2773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the nuclear envelope (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Chertkova AO, Mastop M, Postma M, van Bommel N, van der Niet S, 
            Batenburg KL, Joosen L, Gadella TWJ, Okada Y, Goedhart J
  TITLE     Robust and Bright Genetically Encoded Fluorescent Markers for 
            Highlighting Structures and Compartments in Mammalian Cells
  JOURNAL   bioRxiv (2020) 160374; doi: https://doi.org/10.1101/160374
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) 160374; doi: https://doi.org/10.1101/160374"
COMMENT     Sequence Label: mTurquoise2-LaminB
FEATURES             Location/Qualifiers
     source          1..2773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(42..44,45..47,48..758)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(87..108)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(348..367)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     695..716
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             801..842
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    858..882
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2680..2704)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggggattgc ggtcgatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg
       61 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      121 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      181 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct
      241 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      301 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      361 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      421 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact
      481 ttagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
      541 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
      601 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
      661 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      721 ccgccgggat cactctcggc atggacgagc tgtacaagaa gtccggactc agatctcgag
      781 ctcaagcttc gaattgcggt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
      841 cgatcgattt cgatcaaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca
      901 ccatggcgac tgcgaccccc gtgccgccgc ggatgggcag ccgcgctggc ggccccacca
      961 cgccgctgag ccccacgcgc ctgtcgcggc tccaggagaa ggaggagctg cgcgagctca
     1021 atgaccggct ggcggtgtac atcgacaagg tgcgcagcct ggagacggag aacagcgcgc
     1081 tgcagctgca ggtgacggag cgcgaggagg tgcgcggccg tgagctcacc ggcctcaagg
     1141 cgctctacga gaccgagctg gccgacgcgc gacgcgcgct cgacgacacg gcccgcgagc
     1201 gcgccaagct gcagatcgag ctgggcaagt gcaaggcgga acacgaccag ctgctcctca
     1261 actatgctaa gaaggaatct gatcttaatg gcgcccagat caagcttcga gaatatgaag
     1321 cagcactgaa ttcgaaagat gcagctcttg ctactgcact tggtgacaaa aaaagtttag
     1381 agggagattt ggaggatctg aaggatcaga ttgcccagtt ggaagcctcc ttagctgcag
     1441 ccaaaaaaca gttagcagat gaaactttac ttaaagtaga tttggagaat cgttgtcaga
     1501 gccttactga ggacttggag tttcgcaaaa gcatgtatga agaggagatt aacgagacca
     1561 gaaggaagca tgaaacgcgc ttggtagagg tggattctgg gcgtcaaatt gagtatgagt
     1621 acaagctggc gcaagccctt catgagatga gagagcaaca tgatgcccaa gtgaggctgt
     1681 ataaggagga gctggagcag acttaccatg ccaaacttga gaatgccaga ctgtcatcag
     1741 agatgaatac ttctactgtc aacagtgcca gggaagaact gatggaaagc cgcatgagaa
     1801 ttgagagcct ttcatcccag ctttctaatc tacagaaaga gtctagagca tgtttggaaa
     1861 ggattcaaga attagaggac ttgcttgcta aagaaaaaga caactctcgt cgcatgctga
     1921 cagacaaaga gagagagatg gcggaaataa gggatcaaat gcagcaacag ctgaatgact
     1981 atgaacagct tcttgatgta aagttagccc tggacatgga aatcagtgct tacaggaaac
     2041 tcttagaagg cgaagaagag aggttgaagc tgtctccaag cccttcttcc cgtgtgacag
     2101 tatcccgagc atcctcaagt cgtagtgtac gtacaactag aggaaagcgg aagagggttg
     2161 atgtggaaga atcagaggcg agtagtagtg ttagcatctc tcattccgcc tcagccactg
     2221 gaaatgtttg catcgaagaa attgatgttg atgggaaatt tatccgcttg aagaacactt
     2281 ctgaacagga tcaaccaatg ggaggctggg agatgatcag aaaaattgga gacacatcag
     2341 tcagttataa atatacctca agatatgtgc tgaaggcagg ccagactgtt acaatttggg
     2401 ctgcaaacgc tggtgtcaca gccagccccc caactgacct catctggaag aaccagaact
     2461 cgtggggcac tggcgaagat gtgaaggtta tattgaaaaa ttctcaggga gaggaggttg
     2521 ctcaaagaag tacagtcttt aaaacaacca tacctgaaga agaggaggag gaggaagaag
     2581 cagctggagt ggttgttgag gaagaacttt tccaccagca gggaacccca agagcatcca
     2641 atagaagctg tgcaattatg taaaagggtg ggcgcgccga cccagctttc ttgtacaaag
     2701 tggttcgatc gaaccgcggt ctagactcga cggtaccgcg ggcccgggat ccaccggatc
     2761 tagaaacaat ccc
//
