LOCUS       sequence-198462-        2749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the nuclear envelope (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Chertkova AO, Mastop M, Postma M, van Bommel N, van der Niet S, 
            Batenburg KL, Joosen L, Gadella TWJ, Okada Y, Goedhart J
  TITLE     Robust and Bright Genetically Encoded Fluorescent Markers for 
            Highlighting Structures and Compartments in Mammalian Cells
  JOURNAL   bioRxiv (2020) 160374; doi: https://doi.org/10.1101/160374
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) 160374; doi: https://doi.org/10.1101/160374"
COMMENT     Sequence Label: mScarlet-I-LaminB
FEATURES             Location/Qualifiers
     source          1..2749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..737
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(175..193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     443..462
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             777..818
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    834..858
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2656..2680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggggattgc ggtcgatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg
       61 cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg aacggccacg
      121 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      181 tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc cctcagttca
      241 tgtacggctc cagggccttc atcaagcacc ccgccgacat ccccgactac tataagcagt
      301 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgccgtga
      361 ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg aagctccgcg
      421 gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc tgggaagcat
      481 ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag atggccctgc
      541 gcctgaagga cggcggccgc tacctggcgg acttcaagac cacctacaag gccaagaagc
      601 ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt ggacatcacc tcccacaacg
      661 aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc accggcggca
      721 tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat tgcggtggta
      781 agcctatccc taaccctctc ctcggtctcg attctacgat cgatttcgat caaacaagtt
      841 tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat ggcgactgcg acccccgtgc
      901 cgccgcggat gggcagccgc gctggcggcc ccaccacgcc gctgagcccc acgcgcctgt
      961 cgcggctcca ggagaaggag gagctgcgcg agctcaatga ccggctggcg gtgtacatcg
     1021 acaaggtgcg cagcctggag acggagaaca gcgcgctgca gctgcaggtg acggagcgcg
     1081 aggaggtgcg cggccgtgag ctcaccggcc tcaaggcgct ctacgagacc gagctggccg
     1141 acgcgcgacg cgcgctcgac gacacggccc gcgagcgcgc caagctgcag atcgagctgg
     1201 gcaagtgcaa ggcggaacac gaccagctgc tcctcaacta tgctaagaag gaatctgatc
     1261 ttaatggcgc ccagatcaag cttcgagaat atgaagcagc actgaattcg aaagatgcag
     1321 ctcttgctac tgcacttggt gacaaaaaaa gtttagaggg agatttggag gatctgaagg
     1381 atcagattgc ccagttggaa gcctccttag ctgcagccaa aaaacagtta gcagatgaaa
     1441 ctttacttaa agtagatttg gagaatcgtt gtcagagcct tactgaggac ttggagtttc
     1501 gcaaaagcat gtatgaagag gagattaacg agaccagaag gaagcatgaa acgcgcttgg
     1561 tagaggtgga ttctgggcgt caaattgagt atgagtacaa gctggcgcaa gcccttcatg
     1621 agatgagaga gcaacatgat gcccaagtga ggctgtataa ggaggagctg gagcagactt
     1681 accatgccaa acttgagaat gccagactgt catcagagat gaatacttct actgtcaaca
     1741 gtgccaggga agaactgatg gaaagccgca tgagaattga gagcctttca tcccagcttt
     1801 ctaatctaca gaaagagtct agagcatgtt tggaaaggat tcaagaatta gaggacttgc
     1861 ttgctaaaga aaaagacaac tctcgtcgca tgctgacaga caaagagaga gagatggcgg
     1921 aaataaggga tcaaatgcag caacagctga atgactatga acagcttctt gatgtaaagt
     1981 tagccctgga catggaaatc agtgcttaca ggaaactctt agaaggcgaa gaagagaggt
     2041 tgaagctgtc tccaagccct tcttcccgtg tgacagtatc ccgagcatcc tcaagtcgta
     2101 gtgtacgtac aactagagga aagcggaaga gggttgatgt ggaagaatca gaggcgagta
     2161 gtagtgttag catctctcat tccgcctcag ccactggaaa tgtttgcatc gaagaaattg
     2221 atgttgatgg gaaatttatc cgcttgaaga acacttctga acaggatcaa ccaatgggag
     2281 gctgggagat gatcagaaaa attggagaca catcagtcag ttataaatat acctcaagat
     2341 atgtgctgaa ggcaggccag actgttacaa tttgggctgc aaacgctggt gtcacagcca
     2401 gccccccaac tgacctcatc tggaagaacc agaactcgtg gggcactggc gaagatgtga
     2461 aggttatatt gaaaaattct cagggagagg aggttgctca aagaagtaca gtctttaaaa
     2521 caaccatacc tgaagaagag gaggaggagg aagaagcagc tggagtggtt gttgaggaag
     2581 aacttttcca ccagcaggga accccaagag catccaatag aagctgtgca attatgtaaa
     2641 agggtgggcg cgccgaccca gctttcttgt acaaagtggt tcgatcgaac cgcggtctag
     2701 actcgacggt accgcgggcc cgggatccac cggatctaga aacaatccc
//
