LOCUS       sequence-198494-        5491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBA1
FEATURES             Location/Qualifiers
     source          1..5491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             434..1225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1885..1904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2138..2155
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatgcttca caagcgagct agtgaagcta acgataagaa cgataatacc
     2281 atcatcggtt ccgatttagc atcatctaag aaacgccgta tcgatttcac tgaatcttcg
     2341 tctgataagt catcttcgat tttagcttct ggtagtagca ggggttttca cggcgatagc
     2401 gtcgttcagc aaatcgacat ggcttttggt aattcgaacc gtcaggagat tgatgaagat
     2461 ctgcacagtc gccagctcgc cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc
     2521 aatgttctca tctcggggat gcacggtctt ggtgccgaga ttgccaagaa tcttatactt
     2581 gctggtgtga agtctgtgac cttgcatgat gaaagagttg tagagctatg ggacttatca
     2641 agcaactttg ttttctcgga ggatgatgtt ggcaagaata gagcagatgc ttctgttcag
     2701 aagttacagg atcttaacaa tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa
     2761 gaggatcttt ctggcttcca ggttgttgta ttctctgaca taagcatgga aagagcaatc
     2821 gaatttgatg actattgtca cagccatcag cctcctatcg cttttgtcaa ggctgatgtc
     2881 aggggtcttt tcggctctgt cttttgtgat tttgggcctg aatttgcggt tcttgatgtt
     2941 gatggagagg aaccccatac aggcatcatt gcgtctatct ctaatgagaa ccaggcattt
     3001 atttcctgtg tcgacgatga gaggcttgag tttgaagatg gtgacctagt tgttttctca
     3061 gaagttgagg gtatgacaga attgaatgat gggaaaccaa ggaagataaa aagtacacgg
     3121 ccatattcat tcacccttga tgaggacact actaactatg gaacatatgt gaagggtggt
     3181 attgtcactc aagtgaaaca gccaaagtta ctgaatttca agccattgag ggaagcgctt
     3241 aaggatccag gggattttct atttagtgat ttctctaagt ttgatcggcc tccactcctt
     3301 cacttagcat tccaggcact tgatcatttt aaagctgaag ctggcagatt tcctgttgct
     3361 ggctcagaag aggacgctca gaagcttata tcgattgcca cagccatcaa taccgggcag
     3421 ggtgatttaa aggtggagaa tgttgatcag aagcttctaa gacacttttc ctttggagcc
     3481 aaggctgtcc tgaatcctat ggctgcaatg tttggcggta ttgttggaca ggaggttgtt
     3541 aaagcttgct ctggaaaatt tcatcctctc ttccagtttt tctactttga ttcagtggag
     3601 tcactcccct ctgaacctgt ggactcgagt gactttgcac caagaaacag ccggtatgat
     3661 gcccaaatat ctgtatttgg ggccaagttc cagaagaaac ttgaagatgc taaagttttc
     3721 acagtagggt ctggtgctct tggctgtgag ttcttgaaaa atttggctct gatgggggtt
     3781 tcttgtggaa gccaagggaa gctgacagtg acagatgatg atatcattga gaagagtaac
     3841 ctcagtcgtc aatttctgtt ccgtgactgg aacattggac aggctaaatc cacagttgct
     3901 gcttcggctg ctgctgttat aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt
     3961 ggcgcggaga ctgagaatgt atttgacgat gccttctggg agaacttaac tgttgtcgtc
     4021 aatgcactgg ataatgttaa tgcgaggctc tatgttgatt cgaggtgctt gtatttccag
     4081 aagcctctcc ttgagtctgg aactcttggt acaaagtgca acacacagtc ggttatccca
     4141 catctaactg aaaattacgg tgcctcaagg gacccaccag agaaacaggc ccccatgtgt
     4201 acagtgcatt ccttcccgca taacattgat cactgtttaa cttgggctcg ctctgagttt
     4261 gagggtctgc tggagaaaac tcccgctgaa gtgaatgcat atctttctag cccagttgag
     4321 tacactaact caatgatgag tgctggtgat gctcaggcga gggacacatt ggagaggatt
     4381 gttgagtgcc ttgaaaagga gaagtgtgaa accttccaag actgcttaac ctgggcacga
     4441 ctgaggtttg aggattactt tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa
     4501 gatgctgcga ctagtaccgg agctccattc tggtctgctc caaaaagatt ccctcgtcca
     4561 ctccagtact cgtcgtccga cccaagtctc cttaacttta tcacagcgac tgctatttta
     4621 cgagcagaga catttgggat cccgatacct gagtggacta agaacccaaa ggaagcagca
     4681 gaagctgttg acagagtgat agtcccagac tttgagccaa ggcaagatgc aaagattgtg
     4741 actgatgaga aagctaccac tttaaccact gcttcggtgg acgacgctgc agtcatcgac
     4801 gatctcattg ctaagatcga ccagtgtagg cataacttgt cgccagactt caggatgaaa
     4861 cctattcagt tcgaaaagga tgatgataca aactatcaca tggatgtgat agcaggacta
     4921 gccaacatga gggcgagaaa ttacagcatc cctgaagtgg acaagctgaa agcaaagttc
     4981 atagcaggga gaatcatccc agccattgcg acttcaacag ccatggctac tgggctggtc
     5041 tgcctcgagc tgtacaaggt ccttgatgga ggacataaag tggaagctta cagaaacaca
     5101 tttgccaacc tggcacttcc actattctcc atggctgaac cacttccacc aaaggtggtg
     5161 aagcaccgtg acatggcttg gacagtttgg gacagatggg ttctcaaggg aaacccaact
     5221 cttcgtgagg tgttgcagtg gctggaggac aaagggctta gcgcatacag catctcttgt
     5281 ggaagctgtc tactgttcaa cagtatgttc acaaggcaca aggagaggat ggacaagaag
     5341 gttgtggatc tcgctcggga tgttgcgaaa gtcgagttac cgccttatcg caaccatctt
     5401 gatgtagtgg tggcttgtga ggatgaagat gacaatgacg tcgatattcc tctcgtctct
     5461 atctacttca ggtgagcttt gagaccacga a
//
