LOCUS       sequence-198517-        5557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC23
FEATURES             Location/Qualifiers
     source          1..5557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             434..1225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1885..1904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2138..2155
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatggagca tgagcaagat gaccctggta catcaacaaa tgtaggggtt
     2281 gactcgtcag ttgatgattc aatggcaagt ctctctatat gtgattcaga gcatcctaat
     2341 atttatcgac aagatattgt caaaaacaaa aagaccggta gtgttggtgt tgtcagcgaa
     2401 gtagctggtg attctgactc tgatagtgat attagtgatg aggaagaaga tgatgatgat
     2461 gacgaagata atgatgatga tgatgaggat gtagaggaag ggaaaaaggc tagtgaagaa
     2521 aatgttgtta atggagatgg cgagaaaaaa gctgatggga attataaatg tggtgcactt
     2581 gagggagatc agattcgtgt gctttggatg gataacactg agcctgttca agatattaat
     2641 gatgtaactg ttatagaccg tggtttctta catggtgatt atgttgcttc tgcttctgag
     2701 cctactggtc aggtgggagt tgttgtggat gttaacatat cagttgattt gttggctcct
     2761 gatggttcta ttcacaagga catctctacc aagaatttga aacgcgtcag ggattttgct
     2821 gttggtgatt atgtggttca tggtccatgg ctaggtagaa tcgatgatgt gttggacaat
     2881 gtgactgtct tatttgatga tggttccatg tgtaaagttc tacgtgttga acctcttcga
     2941 cttaaaccta ttccgaagaa taatcttgaa gaagatgcta attttcctta ttacccaggg
     3001 cagcgggtta aagcaagctc ctcttcggtt tttaagaatt cgcggtggtt atctggactg
     3061 tggaagccta atcgtttaga aggtacggtg accaaagtta ctgcgggatc aatttttgtc
     3121 tactggattg cctcagctgg ctttggtcca gattcttctg tttccccacc tgaggagcag
     3181 aatccaagta atttgacatt gttgtcatgt ttcactcatg ccaattggca agtgggtgac
     3241 tggtgccttc ttccatctct gaatcaatct gctacaatcc ccttacacaa gcatgtatct
     3301 aaattaagac tttatgattc ccaagctgat cggcaacaga aaattgggcg tgatttagaa
     3361 gatgtgcaag atgaagtaag tggaaaagtt gaaccagctg gtattactgc tgaagctttg
     3421 ccaaaagtaa ctagtgacga tcctcctcaa agaaatcctt ctgtttcaaa ggagcctgtt
     3481 catgagcctt ggcctctcca tcggaagaag attcgcaaac ttgttatcag aaaggacaaa
     3541 aaggttaaga aaaaagagga aagctttgaa caagctcttt tggtagttaa tagcagaaca
     3601 cgcgttgatg tatcctggca ggatggtaca atagaatgta gacgggaagc gataacattg
     3661 attccaattg agactcctgg tgatcatgaa tttgtctctg agcagtatgt ggtagagagg
     3721 acctctgatg atggtgataa cacaactgaa cctagacgtg ctggcgttgt aaaaaatgtc
     3781 aatgcaaaag atcgtactgc ttctgtgagg tggctaaatc cacttcgaag ggcagaagaa
     3841 ccccgtgagt ttgaaaagga agaaattgtc agtgtgtatg agctagaggg ccatccagat
     3901 tatgactact gttatgggga tgttgttgtt cgcttatcac ctattgccgt tgcgttacca
     3961 gcatcttctc ccggaaactc ttttgaggag gcaacacagc aagacaatgg ataccaagat
     4021 tcagaatccc atcaagaagc taaaatcctt gtggataagg aagaaaacga gcctagtacg
     4081 gacctttcaa agctctcatg ggttggaaat attactggcc tcaaggatgg tgatattgaa
     4141 gtaacatggg ccgatggaac aatatcaacg gttggccctc atgcagttta tgtagttgga
     4201 cgcgatgatg atgatgaatc agttgctgga gaaagtgaaa caagtgatgc tgctagttgg
     4261 gaaactttaa acgatgatga taggggtgct cctgagattc ctgaagagga ccttggaagg
     4321 agtagttcca ttgagggaaa ctctgatgca gatatctacg ctgagaatga ttctgggagg
     4381 aacggtgccc tagctcttcc tctggctgca atcgaatttg tgactcgact ggctagtgga
     4441 atcttttcgc gtgcaaggaa atctgtagat tcttccagtt ctgattacac ggttgaaaat
     4501 gtatataagc aagctgaatc gaccaacccg tccgatgaaa cagattccct tgatgaccct
     4561 agtccttcaa aagttaatgt gactgataat tgcgagtcaa agggcactca agcaaatgcc
     4621 aagaatattt tgagcgggga aacctctaca tttttagaag atgaagataa gccagtacct
     4681 tctgaaggtg atagttgcag tttcagacgc tttgatatat cacaagatcc tctggaccac
     4741 catttccttg gcgtagatgg gcagaaaacc aaagaaagac agtggttcaa gaaggttgat
     4801 caagactgga aaatacttca gaacaatctt ccagatggaa tctttgttcg agcttacgaa
     4861 gatagaatgg atctcttgag ggcagtaata gtcggggcat ttggaacacc ataccaagat
     4921 ggactcttct tttttgattt tcaccttcca tctgactacc ctagtgtgcc accgtcggca
     4981 tattatcatt ctggtggatg gaggttaaac cccaatctgt acgaagaagg caaagtctgt
     5041 cttagccttc tcaatacctg gacaggcagg ggaaatgaag tctgggatcc caaatcatct
     5101 agcatccttc aagttcttgt ttcactccag ggattggtgt tgaattcaaa gccttatttc
     5161 aatgaagccg ggtatgataa gcaggtcgga actgcggaag gagaaaaaaa ctcactggga
     5221 tacaacgaga atactttctt gctaaattgt aaaaccatga tgtatcttat gcggaaacca
     5281 ccaaaggatt ttgaagaact catcaaagat catttcagaa aacgcggata ttacattttg
     5341 aaggcatgtg acgcctacat gaaaggatat ctcataggtt ccctcaccaa agatgcctcg
     5401 gttatcgatg aacgcagcag cgctaattca acttcagttg gttttaagct tatgctggct
     5461 aagatagcac caaagctttt ctcagcactg agcgaggtag gagctgactg caatgaattc
     5521 cagcatctgc agcagcaata agctttgaga ccacgaa
//
