LOCUS       sequence-198518-        4972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC24
FEATURES             Location/Qualifiers
     source          1..4972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             434..1225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1885..1904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2138..2155
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatggaaat gtcccttact gactctgatt gggatagctc cagcgacagt
     2281 ggtagcagtg aacacgaaga agtcgagttt tcttatggtg gacgggcaca gaacattttc
     2341 tcaaaccttg aagaaaccat tggcaaaatc gatgagttcc tgtcgttcga gaggggattt
     2401 atgtatggtg acattgtgcg gtccgccact gaaccatcag gacagagtgg cagggttatc
     2461 aacatagaca tgtttgtcaa tctcgaaagt actcatggga agatcatgaa ggaagttgat
     2521 accaagaggc ttcaaaagtt gcgttctatt tcactctctg attatgtgat taacggacct
     2581 tgggttggaa gggttgacaa aatagttgag cgtgtctctg tcacccttga tgatgggacc
     2641 aactatgagg tccttgtaga tggtcaagat aaacttgtgg ccattccccc aaatttactc
     2701 gaggattctc aatattcgta ttacccaggg caaagagttc aggtaaagct ggcccatgcc
     2761 cccagatcaa ctacatggtt atgcgggacc tggagaggaa cccaggttat gggaactgtt
     2821 tgcactgtag aagcaggact tgtctacgtc gattgggttg cctccatcgt aatggagggt
     2881 gatcggaatt taactgcacc tcaagctttg cagaatcctg agagtttaac tttgttacct
     2941 tgtgtttctc atgcgagttg gcagcttggt gactggtgta tactccctgg ctcttcccac
     3001 tgtgatatag cagagcggca aactccaaat gtggctgcct acaatctcaa tgaatgccat
     3061 aagacattcc aaaaagggtt taacagaaat atgcagaact caggtttgga tgagctattt
     3121 gttatcacaa agacaaagat gaaggttgct gttatgtggc aagatggtag ctgcagtctg
     3181 ggagttgatt cccaacagct gcttcctgtt ggtgctgtta atgctcatga tttttggccc
     3241 gaacagtttg ttgtggaaaa ggaaacctgc aacagcaaaa aatggggagt tgtgaaggct
     3301 gtcaatgcta aggaacaaac tgtgaaggta caatggacaa tacaggttga gaaagaagca
     3361 acaggttgtg ttgatgaggt gatggaagaa attgtcagtg catatgaact gcttgagcac
     3421 cctgattttg gattctgttt cagcgatgtg gtggtcaagt tacttccaga aggaaaattt
     3481 gatccaaatg cagacacaat cgtcgctaca gaggcgaaac acctacttac agagagtgac
     3541 tacagtggcg catatttttt gtcaagtatt ggtgttgtta caggttttaa aaatggttcc
     3601 gtgaaggtga aatgggccaa tggttctact agcaaggttg caccatgtga aatttggaaa
     3661 atggaaaggt ctgaatattc caactctagc actgtaagtt cggaaggcag tgttcaagat
     3721 ctaagtcaga agattagtca atcggatgaa gcatcttcaa accatcagga aacgggtctg
     3781 gtgaagctct acagtgttgg tgaaagttgc aacgagaaca ttccggaatg tagttcattt
     3841 ttccttccaa aagctgccat tggatttatc acaaaccttg catcaagcct ttttggttat
     3901 cagggttcca cttcggttat aagttcacat tcacgttgca atgattctga agatcaaagt
     3961 gactctgagg tccttgttca agaaacagca gaatcatatg acaactctga aacaaattca
     4021 ggtgaagtgg atatgaccac cacgatggtc aacataccta tagaaggaaa aggaattaac
     4081 aagacactgg attcaactct tctagagaac agcaggaacc aagtgagatt cagacagttt
     4141 gatatggtta atgactgctc ggaccatcat ttcctttctt cggataaagg gttggctcag
     4201 tcccaggtca caaagagttg ggtgaagaaa gtccagcaag aatggagcaa tttggaggca
     4261 aatcttccga acacgatata tgtgcgtgtg tgtgaagaaa ggatggacct tctgcgtgca
     4321 gccctggttg gtgcccctgg aacgccatat cacgatgggc ttttcttttt cgacataatg
     4381 cttccacctc aatatcctca tgagccacca atggtacatt atcattcagg tgggatgcga
     4441 ctgaatccga acctgtatga gtcaggaaga gtttgcttga gtctgctgaa tacatggagt
     4501 ggctctggca ctgaagtatg gaacgcaggg agctcctcca tccttcaact tcttctttcg
     4561 tttcaggctc tggttctgaa tgagaagcct tacttcaatg aagctggcta tgataagcag
     4621 ttgggccgag ccgagggaga gaaaaactca gtgagttaca atgagaatgc attcctcata
     4681 acctgcaaat ccatgatctc aatgctccgt aagcctccaa agcattttga gatgcttgtg
     4741 aaggaccatt ttacgcaccg ggcccagcat gttctggctg cgtgcaaggc ttatatggaa
     4801 ggcgtccctg taggatcatc agctaacctg caggggaact caaccacaaa ttccaccggt
     4861 ttcaagatca tgctctccaa actctaccca aaacttcttg aagcattctc agagattgga
     4921 gttgattgcg tccaagagat tggaccagaa tcataagctt tgagaccacg aa
//
