LOCUS       sequence-198519-        4078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC25
FEATURES             Location/Qualifiers
     source          1..4078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             434..1225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1885..1904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2138..2155
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatggagcc caacgtagtg gagatcgcta ctcctcctgc cgcttcttgc
     2281 tcgagaattc gaacgccaac aaaggctgaa actccagaag tgattgatgt ggaggaatat
     2341 gatttgcaaa acggtggtgt tcctaatggc aacaatgttg attataagaa caaagggaag
     2401 gctatagact ttgattccat gtcttacggt gactatggtg aagaagatga gtatgctgtg
     2461 ggatctcctg gtgatgatta tggttatcct gaatcatctc cactatcaaa tagtttgtta
     2521 gacccagagt ctttgatcta tgaggacgac gaaaactatt ccgaacaata tgactttgaa
     2581 atggaggcgg agcctgataa ttactccatg tatcaagacc ttttcgatgg taaggatatc
     2641 ccaactggtg tggaggtttc aatggattgg tttccaaatt cagctgacaa ggaatcagcg
     2701 agtagtagca aatcgtctca tgctaataac gggaataaca gttccaagaa agcaacaaaa
     2761 gcaagtggaa tccattctca attttcttca gatatggaaa cccctgtagc tcaaccatgg
     2821 aatgctttac cgcacaaagc tgaaggagtc ataccaaact ctgcttatgc cttgccacag
     2881 aacagcaagg cttttcaacc accttacgct gtccactatt cagcccttaa aactgctttt
     2941 tccaattatc ttcagcctca gactcctgat accgtattgg gggaggctcc agctcctgct
     3001 gcaggttctt ctggtttatt gccaccaaac acacccggtt ttaaaagtaa tgctgctcgg
     3061 tttaaagagg agccgcctat attacctccg gatgattcca gagttaagag aaatatggag
     3121 gattatcttg ggctttattt gtttttcaaa agatttgaca tcgtcgaaga tttttcagat
     3181 caccactatg cttctaaggg aacaacttca aagcagcatt caaaggattg ggcgaaaagg
     3241 atccaagacg agtggagaat tcttgagaaa gatttaccag agatgatatt tgtcagagct
     3301 tatgaatcta ggatggatct tttaagggct gtaattatcg gagcccaagg aactccctac
     3361 catgatggac tcttcttttt tgatatcttc ttcccggata catacccttc tacgccaccg
     3421 attgttcatt accattctgg tgggctaaga atcaacccaa atctgtacaa ttgtgggaaa
     3481 gtgtgtctga gtctactcgg cacttggagt ggtaaccaga gggagaaatg gatccccaac
     3541 acttcgacga tgctacaagt tcttgtctcg atccaaggtc taatcttaaa tcaaaaacct
     3601 tacttcaacg agcccggcta cgagaggtca gcaggttctg cacatggtga gagtacctct
     3661 aaagcttaca gcgaaaacac attcatactg tctttaaaga ctatggttta caccatgagg
     3721 aggccaccca agtattttga ggactttgcg tatggacatt tcttcagctg tgctcacgac
     3781 gtgttgaagg cgtgtaatgc ttacagaaac ggagctacac cgggatatct ggtgaagggg
     3841 gctccagatg tggaagagaa tagcgcgggc atgagctctc tgaagtttag gacagatgtg
     3901 gctacctttg tggagacagt gctgttgaaa gagtttattc ttctaggtgt ccttggactc
     3961 gaaccggaag aagaagaaaa aacacctgag actataatcg ttgcagagag tagcaaatgt
     4021 accagatcaa ggtccaagag agacagagtt tcttctagtt gagctttgag accacgaa
//
