LOCUS       sequence-198520-        3880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC26
FEATURES             Location/Qualifiers
     source          1..3880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             434..1225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1885..1904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2138..2155
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatggagcc tgacgtggtg gagatccctc ctcctcctct gatcgcttcg
     2281 ggttctagaa ctcgtaaacc cagaaaggct gttcctgaag tgattgatgt ggaaagttat
     2341 gaattccgca atgttggtgt tgttaaggat aacaatgttg ttgataagaa gaacaaaggg
     2401 aaggcaatac aagttgactc cttttctttc aacaacgttc aatctcatca tcatggatcg
     2461 tcgctgctta acttagagac gttccaagac tattatggtc ataagaatat cccttttagt
     2521 gaatttgcta atcaaccaat tgatgttgat gactactcaa tgtatcaaga cgttctagat
     2581 cctaaggatg ttccagctgg tgcagaggtg acggtacctt ggggtttaaa ttcgagcagt
     2641 aagggaactg cgaaatcaag tataagcatt atgagaagtc aatccatgaa aggatatgga
     2701 acagtatctc tggcaacgac taatgttcct caattatggg attatacttt gcctcagcag
     2761 aatcaggcta tctattcttc tgtatcgttt tctgcggttc aacctcagac tcctgatgta
     2821 gtgatggtca ccaatcctac tccaaaccct tttagttatg atgcttctgc tagttcttct
     2881 catcctatag cggctgagcc tatttcttca gttcaggatt cttccaatgc taggaaactt
     2941 aaggaagagt ttctgaggga ttttaaaaga tttgacaccg tcgaggattt ctcagatcat
     3001 cactatgctt caaagggtaa atcttcaaag cagcactcga agaattgggt gaaaaaggtt
     3061 caagcagatt ggaagattct tgagaatgat ttgccagagg caatatctgt cagagcctgt
     3121 gaatcaagaa tggatctttt gagagctgta attattggag ctgagggaac tccttaccat
     3181 gacggtcttt tcttcttcga tattcagttc ccagatactt atccttcagt gccaccgaat
     3241 gttcattacc attctggtgg gcttagaatc aacccgaatc tatacaattg tgggaaagta
     3301 tgcttgagtc tactcggtac ctgggcaggt agcgcgaggg agaaatggct cccaaatgag
     3361 tcgacaatgt tacagcttct tgtctcaatc caagcactca tcttgaatga aaaaccatac
     3421 tttaacgaac ctggctacgt acagagcgcg gggactgcat caggcgagtc caagtctaaa
     3481 gtttacagtg agaacgtatt tctattgtcg ttaaagacta tggtttacag cataagaagg
     3541 ccaccccagc attttgaaga gtatgttcaa aaccattact ttgtccgctc tcacgacatt
     3601 gtgaaagcat gcaatgctta taaagctgga gctccgcttg gatcgatggt taaaggcgga
     3661 gtccaagacc ttgaagaagc tagacagagc ggctccaaga agtttaagac cgatgtagct
     3721 agctttatgc agacagtggt tgatgaattc gtcaaactag gagtcaagga gcttgcagaa
     3781 aaaccagaac cgcctatgag caatgctaac acagagaatc agagcaagaa aaaaacccgt
     3841 aagagaagca gatcatcgag gtgagctttg agaccacgaa
//
