LOCUS       sequence-19853-e         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE
  TITLE     Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for
            protein ISGylation.
  JOURNAL   Mol Cell Biol. 2004 Nov . 24(21):9592-600.
  PUBMED    15485925
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Nov . 24(21):9592-600."
COMMENT     Sequence Label: pCAGGS-FLAG-mISG15
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..36
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     21..37
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(508..531)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(536..552)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(536..552)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(604..623)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ccgggtaccg ggccccccct cgaggtcgac ggtatcgatt tacccacccc tcaggcgcaa
       61 atgcttgatc actgtgcact ggggctttag gccatactcc cccagcagct ccttgtcctc
      121 catgggcctt ccctcgaagc tcagccagaa ctggtcttcg tggacttgtt cccgctggga
      181 caccttcttc ttaagcgtgt ctacagtctg cgtcagaaag acctcataga tgttgctgtg
      241 gcccctttcg ttcctcacca ggatgctcag aggctcgctg cagttctgta ccactagcat
      301 cactgtgctg ctgggaccca gacccagact ggaaagggta agaccgtcct ggagcactgc
      361 agtttggtgg gccaggcgct gctggaaagc cggcacacca atcttctggg caatctgctt
      421 cttcagttct gacaccgtca tggagttagt cacggacacc aggaaatcgt taccccccag
      481 catcttcacc tttaggtccc aggcaagctt gtcgtcatcg tctttgtagt ccatggatcc
      541 actagttcta gagcggccgc caccgcggtg gagctcgaat tctttgccaa aatgatgaga
      601 cagcacaata accagcacgt tgcccaggag ctgtaggaaa a
//
