LOCUS       sequence-19854-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE
  TITLE     Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for
            protein ISGylation.
  JOURNAL   Mol Cell Biol. 2004 Nov . 24(21):9592-600.
  PUBMED    15485925
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Nov . 24(21):9592-600."
COMMENT     Sequence Label: pCAGGS-FLAG-mISG15
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11..27
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             32..55
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(526..542)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(527..543)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(582..601)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    647..702
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 tggcggccgc tctagaacta gtggatccat ggactacaaa gacgatgacg acaagcttgc
       61 ctgggaccta aaggtgaaga tgctgggggg taacgatttc ctggtgtccg tgactaactc
      121 catgacggtg tcagaactga agaagcagat tgcccagaag attggtgtgc cggctttcca
      181 gcagcgcctg gcccaccaaa ctgcagtgct ccaggacggt cttacccttt ccagtctggg
      241 tctgggtccc agcagcacag tgatgctagt ggtacagaac tgcagcgagc ctctgagcat
      301 cctggtgagg aacgaaaggg gccacagcaa catctatgag gtctttctga cgcagactgt
      361 agacacgctt aagaagaagg tgtcccagcg ggaacaagtc cacgaagacc agttctggct
      421 gagcttcgag ggaaggccca tggaggacaa ggagctgctg ggggagtatg gcctaaagcc
      481 ccagtgcaca gtgatcaagc atttgcgcct gaggggtggg taaatcgata ccgtcgacct
      541 cgaggggggg cccggtaccc gggcatgctc gagctagcag atctttttcc ctctgccaaa
      601 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
      661 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcg
//
