LOCUS       sequence-19855-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE
  TITLE     Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for
            protein ISGylation.
  JOURNAL   Mol Cell Biol. 2004 Nov . 24(21):9592-600.
  PUBMED    15485925
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Nov . 24(21):9592-600."
COMMENT     Sequence Label: pCAGGS-5HA-mISG15
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..47
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     32..48
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     5'UTR           58..100
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             136..162
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             166..192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(670..689)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 gctcatcgat gcatggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgctt
       61 gttctttttg cagaagctca gaataaacgc tcaactttgg ccatgtaccc atacgatgtt
      121 ccagattacg ccatgtaccc atacgatgtt ccagattacg ccatgtaccc atacgatgtt
      181 ccagattacg ccatgggtca tatggcctgg gacctaaagg tgaagatgct ggggggtaac
      241 gatttcctgg tgtccgtgac taactccatg acggtgtcag aactgaagaa gcagattgcc
      301 cagaagattg gtgtgccggc tttccagcag cgcctggccc accaaactgc agtgctccag
      361 gacggtctta ccctttccag tctgggtctg ggtcccagca gcacagtgat gctagtggta
      421 cagaactgca gcgagcctct gagcatcctg gtgaggaacg aaaggggcca cagcaacatc
      481 tatgaggtct ttctgacgca gactgtagac acgcttaaga agaaggtgtc ccagcgggaa
      541 caagtccacg aagaccagtt ctggctgagc ttcgagggaa ggcccatgga ggacaaggag
      601 ctgctggggg agtatggcct aaagccccag tgcacagtga tcaagcattt gcgcctgagg
      661 ggtgggggat ctttttccct ctgccaaaat tatggggaca tcatgaagcc ccttgagcat
      721 ctgacttctg gctaataaag gaattttatt ttcattgcaa tagtgtgttg gaatttt
//
