LOCUS       sequence-19856-e         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE
  TITLE     Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for
            protein ISGylation.
  JOURNAL   Mol Cell Biol. 2004 Nov . 24(21):9592-600.
  PUBMED    15485925
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Nov . 24(21):9592-600."
COMMENT     Sequence Label: pCAGGS-6His-mISG15
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(457..474)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(484..501)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             520..542
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(561..583)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cgcaatggct tgatcactgt gcactggggg ctttaggcca tactccccca gcagctcctt
       61 gtcctccatg ggccttccct cgaagctcag ccagaactgg tcttcgtgga cttgttcccg
      121 ctgggacacc ttcttcttaa gcgtgtctac agtctgcgtc agaaagacct catagatgtt
      181 gctgtggccc ctttcgttcc tcaccaggat gctcagaggc tcgctgcagt tctgtaccac
      241 tagcatcact gtgctgctgg gacccagacc cagactggaa agggtaagac cgtcctggag
      301 cactgcagtt tggtgggcca ggcgctgctg gaaagccggc acaccaatct tctgggcaat
      361 ctgcttcttc agttctgaca ccgtcatgga gttagtcacg gacaccagga aatcgttacc
      421 ccccagcatc ttcaccttta ggtcccaggc catatggctg ccgcgcggca ccaggccgct
      481 gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta aagttaaaca
      541 aaattatttc tagagccgcc ggtcacacgc cagaagccga accccgccct gccccgtccc
      601 ccccgaaggc agccgtcccc ccgcggacag ccccgaggct ggagagggag a
//
