LOCUS       sequence-19858-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Kim KI, Zhang DE
  TITLE     UBP43, an ISG15-specific deconjugating enzyme: expression, 
            purification, and enzymatic assays.
  JOURNAL   Methods Enzymol. 2005;398:491-9. doi: 10.1016/S0076-6879(05)98040-3.
  PUBMED    16275353
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2005"; volume: "398:491-9. doi"; pages: " 
            10.1016/S0076-6879(05)98040-3"
COMMENT     Sequence Label: pET15b-mISG15(GG)
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(628..646)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tgagtnattc cctctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggcctggga
      121 cctaaaggtg aagatgctgg ggggtaacga tttcctggtg tccgtgacta actccatgac
      181 ggtgtcagaa ctgaagaagc agattgccca gaagattggt gtgccggctt tccagcagcg
      241 cctggcccac caaactgcag tgctccagga cggtcttacc ctttccagtc tgggtctggg
      301 tcccagcagc acagtgatgc tagtggtaca gaactgcagc gagcctctga gcatcctggt
      361 gaggaacgaa aggggccaca gcaacatcta tgaggtcttt ctgacgcaga ctgtagacac
      421 gcttaagaag aaggtgtccc agcgggaaca agtccacgaa gaccagttct ggctgagctt
      481 cgagggaagg cccatggagg acaaggagct gctgggggag tatggcctaa agccccagtg
      541 cacagtgatc aagcatttgc gcctgagggg tgggtaagga tccggctgct aacaaagccc
      601 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      661 cctctaaacg ggtcttgagg gttttttgnn gaanggagga actatattcc ggtatcccgc
      721 aagaggccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
      781 cnagatgaaa aacctt
//
