LOCUS       sequence-198606-       10563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains human kappa CL domain, for cloning of antibody VL domains 
            using SapI restriction enzyme..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
  TITLE     Expression of IgG Monoclonals with Engineered Immune Effector 
            Functions.
  JOURNAL   Methods Mol Biol. 2018;1827:313-334. doi: 
            10.1007/978-1-4939-8648-4_16.
  PUBMED    30196504
REFERENCE   2  (bases 1 to 10563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1827:313-334. doi"; pages: " 
            10.1007/978-1-4939-8648-4_16"
COMMENT     Sequence Label: pRVL-4
FEATURES             Location/Qualifiers
     source          1..10563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      629..638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1047..1181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1071..1090)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1125..1144
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1324..1346)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1906..3695
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(3997..5922)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6357..6375)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6443..6547
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6548..6616,6617..7408)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6766..6785)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7579..8167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8068..8087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8598..8743
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8786..9823
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(9821..9840)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9865..9913
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgccgc caccatggtg ctgcagaccc aggtgttcat
      661 cagcctgctg ctgtggatca gcggcgccta cggcgaagag ctacagagtg agctcttcag
      721 gaccgtggcc gcccccagcg tgttcatctt cccccccagc gacgagcagc tgaaaagcgg
      781 caccgccagc gtggtgtgcc tgctgaacaa cttctacccc agggaggcca aggtgcagtg
      841 gaaggtggac aacgccctgc agagcggcaa cagccaggag agcgtgaccg agcaggacag
      901 caaggacagc acctacagcc tgagcagcac cctgaccctg agcaaggccg actacgagaa
      961 gcacaaggtg tacgcctgcg aggtgaccca ccagggcctg agcagccccg tgaccaagag
     1021 cttcaacagg ggcgagtgct gataaggatc cagacatgat aagatacatt gatgagtttg
     1081 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1141 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     1201 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     1261 acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga
     1321 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     1381 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     1441 gaggtcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct
     1501 ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca
     1561 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact
     1621 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct
     1681 ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg
     1741 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg
     1801 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa
     1861 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg
     1921 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc
     1981 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat
     2041 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag
     2101 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc
     2161 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg
     2221 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat
     2281 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca
     2341 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata
     2401 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg
     2461 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca
     2521 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag
     2581 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg
     2641 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt
     2701 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata
     2761 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc
     2821 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata
     2881 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
     2941 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
     3001 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     3061 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     3121 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     3181 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     3241 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
     3301 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     3361 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     3421 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     3481 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     3541 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag
     3601 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa
     3661 gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa
     3721 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
     3781 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
     3841 gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca
     3901 ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct
     3961 tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc
     4021 atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc
     4081 caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac
     4141 cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa
     4201 aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct
     4261 tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg
     4321 tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt
     4381 cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc
     4441 tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt
     4501 ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc
     4561 accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc
     4621 tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa
     4681 ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg
     4741 tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc
     4801 acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc
     4861 ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc
     4921 actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc
     4981 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
     5041 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     5101 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     5161 tcctgcccct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc
     5221 ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     5281 tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
     5341 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     5401 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     5461 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
     5521 tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
     5581 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc
     5641 tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt
     5701 ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc
     5761 tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc
     5821 tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc
     5881 taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga
     5941 tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc
     6001 ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg
     6061 aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa
     6121 actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct
     6181 cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt
     6241 caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg
     6301 aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct
     6361 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6421 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6481 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6541 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6601 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6661 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6721 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6781 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6841 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6901 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6961 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7021 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7081 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7141 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7201 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7261 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7321 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7381 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7441 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7501 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7561 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7621 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7681 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7741 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7801 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7861 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7921 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7981 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8041 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8101 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8161 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag
     8221 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     8281 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     8341 cgccagcaag acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg gctgctggag
     8401 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
     8461 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct
     8521 tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc
     8581 atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga
     8641 acacgcagat gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc
     8701 gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc
     8761 cgcagatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     8821 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     8881 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     8941 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     9001 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     9061 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     9121 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     9181 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     9241 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     9301 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     9361 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     9421 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     9481 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     9541 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     9601 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     9661 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     9721 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     9781 ccccagcact cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa
     9841 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
     9901 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     9961 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
    10021 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
    10081 actggccccg tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg
    10141 ttattatttt gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat
    10201 ggtttttgga tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc
    10261 tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag
    10321 ctagtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
    10381 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
    10441 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
    10501 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
    10561 cat
//
