LOCUS       sequence-198608-       11229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of 
            antibody VH domains using SapI restriction enzyme. Abolished 
            Fc-mediated immune effector functions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
  TITLE     Expression of IgG Monoclonals with Engineered Immune Effector 
            Functions.
  JOURNAL   Methods Mol Biol. 2018;1827:313-334. doi: 
            10.1007/978-1-4939-8648-4_16.
  PUBMED    30196504
REFERENCE   2  (bases 1 to 11229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1827:313-334. doi"; pages: " 
            10.1007/978-1-4939-8648-4_16"
COMMENT     Sequence Label: pRVL-6
FEATURES             Location/Qualifiers
     source          1..11229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      629..638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1713..1847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1737..1756)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1791..1810
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1990..2012)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2572..4361
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4663..6588)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7023..7041)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7109..7213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7214..7282,7283..8074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7432..7451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8245..8833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8734..8753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9264..9409
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9452..10489
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10487..10506)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10531..10579
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgccgc caccatggac tggacctgga gggtgttctg
      661 cctgctggcc gtggcccccg gcgcccacag cgaagagcta cagagtgagc tcttcgccag
      721 caccaaaggt ccatcagtat tcccactcgc gccaagctcc aaatccacaa gcggggggac
      781 tgcagctctg ggatgcctcg tcaaagatta tttccccgag cccgtgactg tgagttggaa
      841 ttctggagca ttgaccagtg gcgttcacac gtttccagcg gttctccagt cctctggctt
      901 gtattccctt tctagtgtcg ttaccgtccc atccagcagt cttggcacac aaacttacat
      961 ttgcaatgta aatcacaagc cctcaaatac gaaggttgat aagaaggtgg aacccaaatc
     1021 atgcgacaag acacacactt gtcccccttg ccctgcacct gaagctgaag gggcgccctc
     1081 tgtgtttctg tttccaccga agccaaagga cacgcttatg atttcccgaa cacctgaggt
     1141 tacttgcgtc gttgttgacg tctcccatga agatccagaa gtgaaattca attggtatgt
     1201 tgacggcgtt gaggtccata atgcgaagac taagccccgg gaggagcagt ataacagcac
     1261 ctacagagtt gtatccgtgc ttacggtact tcatcaagat tggttgaacg ggaaagagta
     1321 taaatgtgcc gtctccaata aggctctgcc tgcgtctatt gaaaagacca tttcaaaggc
     1381 caaaggtcaa ccccgggagc cccaagtata tacattgccc ccctctcgag acgagttgac
     1441 taagaatcaa gtcagcttga catgcttggt caaaggtttt tacccttcag atatagcggt
     1501 agagtgggag tccaacggcc agcctgaaaa caactacaag actacccccc ctgtcctgga
     1561 tagcgatgga tctttctttc tgtatagtaa actgaccgtt gataagagta gatggcagca
     1621 aggcaatgtc ttctcttgtt ctgtgatgca cgaggctttg cataatcact atacgcagaa
     1681 aagcttgtct cttagcccgg gaaaatgata aggatccaga catgataaga tacattgatg
     1741 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     1801 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     1861 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     1921 acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga
     1981 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     2041 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     2101 agccatgagg tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg
     2161 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     2221 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     2281 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     2341 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
     2401 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     2461 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     2521 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     2581 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     2641 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     2701 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     2761 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     2821 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
     2881 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
     2941 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
     3001 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
     3061 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
     3121 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
     3181 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
     3241 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
     3301 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
     3361 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
     3421 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
     3481 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
     3541 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
     3601 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
     3661 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     3721 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     3781 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     3841 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     3901 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
     3961 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     4021 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     4081 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     4141 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     4201 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
     4261 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
     4321 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta
     4381 ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg
     4441 tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat
     4501 gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga
     4561 aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac
     4621 acggctttta atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc
     4681 accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc
     4741 cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac
     4801 agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc
     4861 cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga
     4921 ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact
     4981 caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag
     5041 ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt
     5101 cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt
     5161 cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc
     5221 tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc
     5281 ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta
     5341 ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct
     5401 gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc
     5461 acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc
     5521 tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg
     5581 gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc
     5641 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     5701 tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     5761 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
     5821 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
     5881 tcctgcccct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     5941 tcctgctcct gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc
     6001 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc
     6061 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     6121 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     6181 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
     6241 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
     6301 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
     6361 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
     6421 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
     6481 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
     6541 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
     6601 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
     6661 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
     6721 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
     6781 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
     6841 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
     6901 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
     6961 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
     7021 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7081 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7201 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7261 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7321 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7381 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7441 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     7501 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7561 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7621 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7681 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7741 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7801 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7861 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7921 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7981 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8041 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8101 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8161 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8341 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     8401 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8461 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8521 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8581 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8641 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8701 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8761 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8821 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8881 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
     8941 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
     9001 cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg
     9061 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     9121 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     9181 cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
     9241 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
     9301 attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg
     9361 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca
     9421 gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
     9481 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     9541 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     9601 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     9661 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     9721 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     9781 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     9841 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     9901 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     9961 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
    10021 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
    10081 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
    10141 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
    10201 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
    10261 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
    10321 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
    10381 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
    10441 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg
    10501 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
    10561 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
    10621 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
    10681 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
    10741 atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
    10801 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
    10861 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
    10921 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt
    10981 gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
    11041 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
    11101 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
    11161 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
    11221 gaccgccat
//
