LOCUS       sequence-19866-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Zou W, Papov V, Malakhova O, Kim KI, Dao C, Li J, Zhang DE
  TITLE     ISG15 modification of ubiquitin E2 Ubc13 disrupts its ability to 
            form thioester bond with ubiquitin.
  JOURNAL   Biochem Biophys Res Commun. 2005 Oct 14. 336(1):61-8.
  PUBMED    16122702
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2005 Oct 14. 336(1):61-8."
COMMENT     Sequence Label: pcDNA3.0-HA-UbcH13
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(551..569)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(552..569)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(589..606)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    595..706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgacgtccc agactacgct ggaagcttgg taccgatggc cgggctgccc cgcaggatca
       61 tcaaggaaac ccagcgtttg ctggcagaac cagttcctgg catcaaagcc gaaccagatg
      121 agagcaacgc ccgttatttt catgtggtca ttgctggccc tcaggattcc ccctttgagg
      181 gagggacttt taaacttgaa ctattccttc cagaagaata cccaatggca gcccctaaag
      241 tacgtttcat gaccaaaatt tatcatccta atgtagacaa gttgggaaga atatgtttag
      301 atattttgaa agataagtgg tccccagcac tgcagatccg cacagttctg ctatcgatcc
      361 aggccttgtt aagtgctccc aatccagatg atccattagc aaatgatgta gcggagcagt
      421 ggaagaccaa cgaagcccaa gccatagaaa cagctagagc atggactagg ctatatgcca
      481 tgaataatat ttaagcggcc gcgatccatc acactggcgg ccgctcgagc atgcatctag
      541 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
      601 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      661 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tg
//
