LOCUS       sequence-198663-        5767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5767)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-VASP-5
FEATURES             Location/Qualifiers
     source          1..5767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     954..973
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1148..1171
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2495..2616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2532..2551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2586..2605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2623..3078)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2760..2781)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2972..2991
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3105..3209
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3211..3568
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3211..3402
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3234..3254)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3419..3554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3603..4397
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3603..4397
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3657..3676)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4267..4286
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4585..4604)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4629..4676
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5005..5593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5494..5513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtggcta ccatggtgag caagggcgag gaggtcatca aggagttcat gcgcttcaag
      601 gagcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      661 cgcccctacg agggcaccca gaccgccagg ctgaaggtga ccaagggtgg ccccctgccc
      721 ttcgcctggg acatcctgtc ccctcagatc atgtacggct ccaaggccta cgtgaagcac
      781 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      841 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      901 ggcgagttca tctacaaggt gaaggtgcgc ggcaccaact tcccctccga cggccccgta
      961 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1021 gccctgaagg gcgagatgaa gatgaggctg aggctgaagg acggcggcca ctacgacgcc
     1081 gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc
     1141 gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     1201 cgcgccgagg gccgccactc caccggcgcc tccggactca gatctatgag cgagacggtc
     1261 atctgttcca gccgggctac tgtgatgctt tatgatgaca gcaacaagcg atggctccct
     1321 gctggcactg gtccccaggc cttcagccgc gtccagatat accacaaccc cactgctaac
     1381 tccttccgag ttgttggccg caagatgcag ccggatcagc aggtggttat caactgtgcc
     1441 atcattcggg gtgtcaagta caatcaggcc actcccatct tccatcagtg gcgagatgcc
     1501 cgccaggtct ggggcctcaa cttcggcagc aaggaggacg ccatacagtt tgcaacaggc
     1561 atggccaacg ccctagaggc cttggaagga ggtgggcctc ccccagcccc agcaccccct
     1621 gcctggtctg cccagaatgg tccctcccca gaggagctgg aacaacagaa aaggcagccg
     1681 gagcatatgg agcgccgggt ctccaatgca ggaggcccac ctgctccccc agctgggggc
     1741 cctcctccac ctccaggacc tccccctcct ccaggtcccc ccccaccccc aggtctgccc
     1801 tcctcagggg tatctggggc aggtcatgga gcaggggcag ccccaccccc tgcaccccca
     1861 ctccctacaa cacagggccc caatagtggg ggttccgggg ccccaggcct ggctgctgcc
     1921 attgctggag ccaaactcag gaaagtgagc aagcaggagg aggcctctgg ggggcccctg
     1981 gcccccaaag ctgagaacag tcgaagcact ggtggggggc ttatggaaga gatgaacgcc
     2041 atgctggccc ggagaagaaa agccacacag gttggggaga agccccccaa agacgagtca
     2101 gccagtgagg agtcagaggc ccgactccct gcccagagtg aacctgtgag aagaccctgg
     2161 gagaagaaca gcacaacctt gccaaggatg aagtcgtcct cttctgtgac tacctccgag
     2221 gcccacccct cgacgccctg ctccagtgat gactccgact tggagagggt gaagcaggag
     2281 cttctggaag aggtgcggaa ggagctacag aaaatgaaag aggaaatcat cgaagtcttt
     2341 gtccaggagc tgaggaagcg gggttctcct tgagcggccg cgactctaga tcataatcag
     2401 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2461 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2521 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2581 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     2641 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     2701 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     2761 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     2821 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     2881 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     2941 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3001 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3061 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3181 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3241 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3301 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3361 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3421 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3481 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3541 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     3601 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3661 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3721 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3781 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3841 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3901 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3961 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4021 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4081 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4141 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4201 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4261 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4321 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4381 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4441 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4501 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4561 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4621 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     4681 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4741 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     4801 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     4861 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4921 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4981 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5041 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5101 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5161 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5221 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5281 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5341 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5401 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5461 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5521 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5581 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5641 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5701 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     5761 agttccg
//
