LOCUS       sequence-198673-        6426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6426)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..6426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1233
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     polyA_signal    3154..3275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3191..3210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3245..3264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3282..3737)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3419..3440)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3631..3650
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3764..3868
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3870..4227
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3870..4061
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3893..3913)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4078..4213
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4262..5056
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4262..5056
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4316..4335)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4926..4945
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5244..5263)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5288..5335
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5664..6252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6153..6172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgaacag cctgatcaaa gaaaacatgc ggatgaaggt ggtgctggaa
      601 ggcagcgtga acggccacca gttcaagtgc accggcgagg gcgagggcaa cccctacatg
      661 ggcacccaga ccatgcggat caaagtgatc gagggcggac ctctgccctt cgccttcgac
      721 atcctggcca catccttcat gtacggcagc cggaccttca tcaagtaccc caagggcatc
      781 cccgatttct tcaagcagag cttccccgag ggcttcacct gggagagagt gaccagatac
      841 gaggacggcg gcgtgatcac cgtgatgcag gacaccagcc tggaagatgg ctgcctggtg
      901 taccatgccc aggtcagggg cgtgaatttt cccagcaacg gcgccgtgat gcagaagaaa
      961 accaagggct gggagcccaa caccgagatg atgtaccccg ctgacggcgg actgagaggc
     1021 tacacccaca tggccctgaa ggtggacggc ggagggcacc tgagctgcag cttcgtgacc
     1081 acctaccgat ccaagaaaac cgtgggcaac atcaagatgc ccggcatcca cgccgtggac
     1141 caccggctgg aaaggctgga agagtccgac aacgagatgt tcgtggtgca gcgggagcac
     1201 gccgtggcca agttcgccgg cctgggcgga gggtccggac tcagatctcg agctcaagct
     1261 tcgatggcga ctgcgacccc cgtgccgccg cggatgggca gccgcgctgg cggccccacc
     1321 acgccgctga gccccacgcg cctgtcgcgg ctccaggaga aggaggagct gcgcgagctc
     1381 aatgaccggc tggcggtgta catcgacaag gtgcgcagcc tggagacgga gaacagcgcg
     1441 ctgcagctgc aggtgacgga gcgcgaggag gtgcgcggcc gtgagctcac cggcctcaag
     1501 gcgctctacg agaccgagct ggccgacgcg cgacgcgcgc tcgacgacac ggcccgcgag
     1561 cgcgccaagc tgcagatcga gctgggcaag tgcaaggcgg aacacgacca gctgctcctc
     1621 aactatgcta agaaggaatc tgatcttaat ggcgcccaga tcaagcttcg agaatatgaa
     1681 gcagcactca attcgaaaga tgcagctctt gctactgcac ttggtgacaa aaaaagttta
     1741 gagggagatt tggaggatct gaaggatcag attgcccagt tggaagcctc cttagctgca
     1801 gccaaaaaac agttagcaga tgaaacttta cttaaagtag atttggagaa tcgttgtcag
     1861 agccttactg aggacttgga gtttcgcaaa agcatgtatg aagaggagat taacgagacc
     1921 agaaggaagc atgaaacgcg cttggtagag gtggattctg ggcgtcaaat tgagtatgag
     1981 tacaagctgg cgcaagccct tcatgagatg agagagcaac atgatgccca agtgaggctg
     2041 tataaggagg agctggagca gacttaccat gccaaacttg agaatgccag actgtcatca
     2101 gagatgaata cttctactgt caacagtgcc agggaagaac tgatggaaag ccgcatgaga
     2161 attgagagcc tttcatccca gctttctaat ctacagaaag agtctagagc atgtttggaa
     2221 aggattcaag aattagagga cttgcttgct aaagaaaaag acaactctcg tcgcatgctg
     2281 acagacaaag agagagagat ggcggaaata agggatcaaa tgcagcaaca gctgaatgac
     2341 tatgaacagc ttcttgatgt aaagttagcc ctggacatgg aaatcagtgc ttacaggaaa
     2401 ctcttagaag gcgaagaaga gaggttgaag ctgtctccaa gcccttcttc ccgtgtgaca
     2461 gtatcccgag catcctcaag tcgtagtgta cgtacaacta gaggaaagcg gaagagggtt
     2521 gatgtggaag aatcagaggc gagtagtagt gttagcatct ctcattccgc ctcagccact
     2581 ggaaatgttt gcatcgaaga aattgatgtt gatgggaaat ttatccgctt gaagaacact
     2641 tctgaacagg atcaaccaat gggaggctgg gagatgatca gaaaaattgg agacacatca
     2701 gtcagttata aatatacctc aagatatgtg ctgaaggcag gccagactgt tacaatttgg
     2761 gctgcaaacg ctggtgtcac agccagcccc ccaactgacc tcatctggaa gaaccagaac
     2821 tcgtggggca ctggcgaaga tgtgaaggtt atattgaaaa attctcaggg agaggaggtt
     2881 gctcaaagaa gtacagtctt taaaacaacc atacctgaag aagaggagga ggaggaagaa
     2941 gcagctggag tggttgttga ggaagaactt ttccaccagc agggaacccc aagagcatcc
     3001 aatagaagct gtgcaattat gtagggatcc accggatcta gataactgat cataatcagc
     3061 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3121 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3181 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3241 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     3301 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3361 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3421 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3481 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3541 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3601 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3661 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3721 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3841 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     3901 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3961 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4021 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4081 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4141 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4201 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     4261 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4321 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4381 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4441 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4501 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4561 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4621 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4681 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4741 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4801 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4861 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4921 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4981 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5041 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5101 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5161 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5221 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     5281 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     5341 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     5401 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     5461 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     5521 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5581 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5641 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5701 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5761 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5821 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5881 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5941 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6001 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6061 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6121 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6181 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6241 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6301 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6361 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     6421 gttccg
//
