LOCUS       sequence-198674-        6258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6258)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-FYN2-N-10
FEATURES             Location/Qualifiers
     source          1..6258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2184..2864
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2986..3107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3023..3042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3077..3096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3114..3569)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3251..3272)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3463..3482
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3596..3700
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3702..4059
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3702..3893
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3725..3745)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3910..4045
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4094..4888
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4094..4888
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4148..4167)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4758..4777
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5076..5095)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5120..5167
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5496..6084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5985..6004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgggctg tgtgcaatgt aaggataaag aagcaacaaa actgacggag gagagggacg
      601 gcagcctgaa ccagagctct gggtaccgct atggcacaga ccccacccct cagcactacc
      661 ccagcttcgg tgtgacctcc atccccaact acaacaactt ccacgcagcc gggggccaag
      721 gactcaccgt ctttggaggt gtgaactctt cgtctcatac ggggaccttg cgtacgagag
      781 gaggaacagg agtgacactc tttgtggccc tttatgacta tgaagcacgg acagaagatg
      841 acctgagttt tcacaaagga gaaaaatttc aaatattgaa cagctcggaa ggagattggt
      901 gggaagcccg ctccttgaca actggagaga caggttacat tcccagcaat tatgtggctc
      961 cagttgactc tatccaggca gaagagtggt actttggaaa acttggccga aaagatgctg
     1021 agcgacagct attgtccttt ggaaacccaa gaggtacctt tcttatccgc gagagtgaaa
     1081 ccaccaaagg tgcctattca ctttctatcc gtgattggga tgatatgaaa ggagaccatg
     1141 tcaaacatta taaaattcgc aaacttgaca atggtggata ctacattacc acccgggccc
     1201 agtttgaaac acttcagcag cttgtacaac attactcaga gagagctgca ggtctctgct
     1261 gccgcctagt agttccctgt cacaaaggga tgccaaggct taccgatctg tctgtcaaaa
     1321 ccaaagatgt ctgggaaatc cctcgagaat ccctgcagtt gatcaagaga ctgggaaatg
     1381 ggcagtttgg ggaagtatgg atgggtacct ggaatggaaa cacaaaagta gccataaaga
     1441 ctcttaaacc aggcacaatg tcccccgaat cattccttga ggaagcgcag atcatgaaga
     1501 agctgaagca cgacaagctg gtccagctct atgcagtggt gtctgaggag cccatctaca
     1561 tcgtcaccga gtatatgaac aaaggaagtt tactggattt cttaaaagat ggagaaggaa
     1621 gagctctgaa attaccaaat cttgtggaca tggcagcaca ggtggctgca ggaatggctt
     1681 acatcgagcg catgaattat atccatagag atctgcgatc agcaaacatt ctagtgggga
     1741 atggactcat atgcaagatt gctgacttcg gattggcccg attgatagaa gacaatgagt
     1801 acacagcaag acaaggtgca aagttcccca tcaagtggac ggcccccgag gcagccctgt
     1861 acgggaggtt cacaatcaag tctgacgtgt ggtcttttgg aatcttactc acagagctgg
     1921 tcaccaaagg aagagtgcca tacccaggca tgaacaaccg ggaggtgctg gagcaggtgg
     1981 agcgaggcta caggatgccc tgcccgcagg actgccccat ctctctgcat gagctcatga
     2041 tccactgctg gaaaaaggac cctgaagaac gccccacttt tgagtacttg cagagcttcc
     2101 tggaagacta ctttaccgcg acagagcccc agtaccaacc tggtgaaaac ctggcatcca
     2161 gcggggatcc accggtcgcc accatgagtg cgattaagcc agacatgaag atcaaactcc
     2221 gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt acaggcaagc
     2281 cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct ctgccttttg
     2341 cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc aaatatccag
     2401 acaacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct
     2461 tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg gaaggggaca
     2521 ctttctataa taaagttcga ttttatggta ccaactttcc cgccaatggt ccagttatgc
     2581 agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc
     2641 tgacgggtga tattcatatg gctttgttgc ttgaaggaaa tgcccattac cgatgtgact
     2701 tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac cactttgtgg
     2761 accactgcat tgagatttta agccatgaca aagattacaa caaggttaag ctgtatgagc
     2821 atgctgttgc tcattctgga ttgcctgaca atgccagacg ataagcggcc gcgactctag
     2881 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2941 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     3001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     3121 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3181 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3241 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3301 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3361 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3421 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3481 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3541 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3661 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     3721 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3781 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3901 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3961 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4021 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     4081 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4141 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4201 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4261 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4321 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4381 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4441 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4501 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4561 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4621 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4681 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4741 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4801 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4861 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4921 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4981 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5041 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     5101 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     5161 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     5221 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     5281 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     5341 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5401 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5461 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5521 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5581 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5641 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5701 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5761 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5821 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5881 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5941 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6001 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6061 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6121 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6181 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     6241 cccatatatg gagttccg
//
