LOCUS       sequence-198693-       10062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Halo-Actin reporter for detection of both mRNA and protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Yoon YJ, Wu B, Buxbaum AR, Das S, Tsai A, English BP, Grimm JB, 
            Lavis LD, Singer RH
  TITLE     Glutamate-induced RNA localization and translation in neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6877-E6886. Epub 2016 
            Oct 17.
  PUBMED    27791158
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2016-11-1"; volume: "113(44)"; pages: 
            "E6877-E6886. Epub 2016 Oct 17"
COMMENT     Sequence Label: HaloTag-bActinCDS-bActinUTR-MS2V5
FEATURES             Location/Qualifiers
     source          1..10062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2664..3551
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     misc_feature    6452..7040
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6505..6525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6923..6934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7115..7348
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7467..7485)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7553..7657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7658..7726,7727..8518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7876..7895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8689..9277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9178..9197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9431..9448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9688..9709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9724..9729,9730..9747,9748..9754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9762..9778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9767..9789
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9786..9802
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9786..9802
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9852..9871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gattacaagg
     2641 atgacgacga taagggcgga ccggcagaaa tcggtactgg ctttccattc gacccccatt
     2701 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
     2761 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
     2821 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
     2881 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
     2941 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
     3001 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
     3061 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
     3121 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
     3181 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
     3241 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
     3301 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
     3361 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
     3421 gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc
     3481 tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg tctactctgg
     3541 agatttccgg tggaggcggt ggatcaggtg gaggaggttc actgcaggat gatgatatcg
     3601 ccgcgctcgt cgtcgacaac ggctccggca tgtgcaaggc cggcttcgcg ggcgacgatg
     3661 ccccccgggc cgtcttcccc tccatcgtgg ggcgccccag gcaccagggc gtgatggtgg
     3721 gcatgggtca gaaggattcc tatgtgggcg acgaggccca gagcaagaga ggcatcctca
     3781 ccctgaagta ccccatcgag cacggcatcg tcaccaactg ggacgacatg gagaaaatct
     3841 ggcaccacac cttctacaat gagctgcgtg tggctcccga ggagcacccc gtgctgctga
     3901 ccgaggcccc cctgaacccc aaggccaacc gcgagaagat gacccagatc atgtttgaga
     3961 ccttcaacac cccagccatg tacgttgcta tccaggctgt gctatccctg tacgcctctg
     4021 gccgtaccac tggcatcgtg atggactccg gtgacggggt cacccacact gtgcccatct
     4081 acgaggggta tgccctcccc catgccatcc tgcgtctgga cctggctggc cgggacctga
     4141 ctgactacct catgaagatc ctcaccgagc gcggctacag cttcaccacc acggccgagc
     4201 gggaaatcgt gcgtgacatt aaggagaagc tgtgctacgt cgccctggac ttcgagcaag
     4261 agatggccac ggctgcttcc agctcctccc tggagaagag ctacgagctg cctgacggcc
     4321 aggtcatcac cattggcaat gagcggttcc gctgccctga ggcactcttc cagccttcct
     4381 tcctgggcat ggagtcctgt ggcatccacg aaactacctt caactccatc atgaagtgtg
     4441 acgtggacat ccgcaaagac ctgtacgcca acacagtgct gtctggcggc accaccatgt
     4501 accctggcat tgccgacagg atgcagaagg agatcactgc cctggcaccc agcacaatga
     4561 agatcaagat cattgctcct cctgagcgca agtactccgt gtggatcggc ggctccatcc
     4621 tggcctcgct gtccaccttc cagcagatgt ggatcagcaa gcaggagtat gacgagtccg
     4681 gcccctccat cgtccaccgc aaatgcttct aggctagcta ggcggactgt tactgagctg
     4741 cgttttacac cctttctttg acaaaaccta acttgcgcag aaaaaaaaaa aataagagac
     4801 aacattggca tggctttgtt tttttaaatt ttttttaaag tttttttttt tttttttttt
     4861 tttttaagtt tttttgtttt gttttggcgc ttttgactca ggatttaaaa actggaacgg
     4921 tgaaggcgac agcagttggt tggagcaaac atcccccaaa gttctacaaa tgtggctgag
     4981 gactttgtac attgttttgt tttttttttt tttggttttg tcttttttta atagtcattc
     5041 caagtatcca tgaaataagt ggttacagga agtccctcac cctcccaaaa gccaccccca
     5101 ctcctaagag gaggatggtc gcgtccatgc cctgagtcca ccccggggaa ggtgacaacc
     5161 ggtaacctac aaacgggtgg aggatcaccc cacccgacac ttcacaatca aggggtacaa
     5221 tacacaaggg tggaggaaca ccccaccctc cagacacatt acacagaaat ccaatcaaac
     5281 agaagcacca tcagggcttc tgctaccaaa tttatctcaa aaaactacaa caaggaatca
     5341 ccatcaggga ttccctgtgc aatatacgtc aaacgagggc cacgacggga ggacgatcac
     5401 gcctcccgaa tatcggcatg tctggctttc gaattcagtg cgtggagcat cagcccacgc
     5461 agccaatcag agtcgaatac aagtcgactt tcgcgaagag catcagcctt cgcgccattc
     5521 ttacacaaac cacactctcc cctacaggaa cagcatcagc gttcctgccc agtacccaac
     5581 tcaagaaaat ttatgtcccc atgcagcatc agcgcatggg ccccaagaat acatccccaa
     5641 caaaatcaca tccgagcacc aacagggctc ggagtgttgt ttcttgtcca actggacaaa
     5701 ccctccatgg accatcaggc catggactct caccaacaag acaaaaacta ctcttctcga
     5761 agcagcatca gcgcttcgaa acactcgagc atacattgtg cctatttctt gggtggacga
     5821 tcacgccacc catgctctca cgaatttcaa aacacggaca aggacgagca ccaccagggc
     5881 tcgtcgttcc acgtccaata cgattactta cctttcggga tcacgatcac ggatcccgca
     5941 gctacatcac ttccactcag gacattcaag catgcacgat cacggcatgc tccacaagtc
     6001 tcaaccacag aaactaccaa atgggttcag caccagcgaa cccactccta cctcaaacct
     6061 cttcccacaa aactggcaag caggatcacc gcttgcccat tccaacatac caaatcaaaa
     6121 acaattactg gtacagcatc agcgtaccag cccacatctc tcactactat caaaaaccaa
     6181 accgttcagc aacagcgaac ggtacacacg gaaaaatcaa ctggtttaca aatacgaaag
     6241 acgatcacgc tttcgtccag cgcaaactat tacgaaaaac atccgacggg aagagcaaca
     6301 gccttcccgc ggcggaaaac ctcacaaaaa cacgacaaac ggatgcacga acacggcatc
     6361 cgccgacaac ccacaaactt acaaccaggc aaacggtgca ggatcaccgc accgtacatc
     6421 aaacacctca gatctcatat cgatagatcc taatcaacct ctggattaca aaatttgtga
     6481 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6541 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6601 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6661 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6721 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6781 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6841 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6901 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6961 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7021 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
     7081 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7141 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7201 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7261 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7321 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7381 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
     7441 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7501 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7561 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7621 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7681 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7741 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7801 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7861 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     7921 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7981 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8041 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8101 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8161 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8221 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8281 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8341 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8401 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8461 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8521 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8581 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8641 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8701 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8761 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8821 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8881 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8941 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9001 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9061 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9301 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9361 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9421 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9481 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
     9541 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9601 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
     9661 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9721 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9781 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
     9841 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     9901 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     9961 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    10021 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
