LOCUS       sequence-198702-        9704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EBNA episome plasmid for CAG promoter constitutive expression of 
            dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53
            shRNA expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: pCXLE-dCas9VPP300-T2A-GFP-shP53
FEATURES             Location/Qualifiers
     source          1..9704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..27
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             31..51
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(61..84,85..87,88..2643,2644..2646,2647..4161)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(61..84,85..87,88..2574,2575..2577,2578..4161)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4165..4185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4222..4332
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4384..4494
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4546..4656
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4708..4818
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4867..6717
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      6481..6490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6733..6786
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6793..6795,6796..6798,6799..7512)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6793..6795,6796..6798,6799..7509)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7099..7118)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7446..7467
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7550..8138
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7603..7623)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8021..8032)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8229..8248)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8294..8349
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8348..8367)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8710..8726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8710..8726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8723..8745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8734..8750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8758..8764,8765..8782,8783..8788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8803..8824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    8913..8946
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tacccatacg atgttccaga ttacgcttcg ccgaagaaaa agcgcaaggt cgaagcgtcc
       61 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
      121 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
      181 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
      241 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
      301 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
      361 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
      421 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
      481 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
      541 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
      601 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
      661 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
      721 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg
      781 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
      841 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
      901 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
      961 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     1021 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     1081 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     1141 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     1201 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     1261 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     1321 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     1381 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     1441 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     1501 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     1561 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     1621 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     1681 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     1741 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     1801 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     1861 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     1921 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     1981 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     2041 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     2101 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     2161 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     2221 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     2281 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     2341 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     2401 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     2461 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     2521 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt cgacgccatc
     2581 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     2641 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     2701 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     2761 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     2821 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     2881 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     2941 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     3001 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     3061 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     3121 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     3181 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     3241 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     3301 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     3361 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     3421 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     3481 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     3541 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     3601 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     3661 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     3721 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     3781 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     3841 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     3901 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     3961 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     4021 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     4081 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     4141 ctgtctcagc tgggaggcga cagccccaag aagaagagaa aggtggaggc cagcctcgag
     4201 tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat gctcccagcc
     4261 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     4321 cttgacatgc tccccgggtc actacccagc gctagcgtcg agtgcgaaag cggcgggccg
     4381 gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
     4441 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
     4501 tcactaccca gcgctagcgt cgagtgcgaa agcggcgggc cggccgacgc ccttgacgat
     4561 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     4621 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtcactacc cagcgctagc
     4681 gtcgagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
     4741 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     4801 tttgaccttg acatgctccc cgggtcacta cccagcgcta gcgtcgagtt cgaaggcagc
     4861 ggcgggattt tcaaaccaga agaactacga caggcactga tgccaacttt ggaggcactt
     4921 taccgtcagg atccagaatc ccttcccttt cgtcaacctg tggaccctca gcttttagga
     4981 atccctgatt actttgatat tgtgaagagc cccatggatc tttctaccat taagaggaag
     5041 ttagacactg gacagtatca ggagccctgg cagtatgtcg atgatatttg gcttatgttc
     5101 aataatgcct ggttatataa ccggaaaaca tcacgggtat acaaatactg ctccaagctc
     5161 tctgaggtct ttgaacaaga aattgaccca gtgatgcaaa gccttggata ctgttgtggc
     5221 agaaagttgg agttctctcc acagacactg tgttgctacg gcaaacagtt gtgcacaata
     5281 cctcgtgatg ccacttatta cagttaccag aacaggtatc atttctgtga gaagtgtttc
     5341 aatgagatcc aaggggagag cgtttctttg ggggatgacc cttcccagcc tcaaactaca
     5401 ataaataaag aacaattttc caagagaaaa aatgacacac tggatcctga actgtttgtt
     5461 gaatgtacag agtgcggaag aaagatgcat cagatctgtg tccttcacca tgagatcatc
     5521 tggcctgctg gattcgtctg tgatggctgt ttaaagaaaa gtgcacgaac taggaaagaa
     5581 aataagtttt ctgctaaaag gttgccatct accagacttg gcacctttct agagaatcgt
     5641 gtgaatgact ttctgaggcg acagaatcac cctgagtcag gagaggtcac tgttagagta
     5701 gttcatgctt ctgacaaaac cgtggaagta aaaccaggca tgaaagcaag gtttgtggac
     5761 agtggagaga tggcagaatc ctttccatac cgaaccaaag ccctctttgc ctttgaagaa
     5821 attgatggtg ttgacctgtg cttctttggc atgcatgttc aagagtatgg ctctgactgc
     5881 cctccaccca accagaggag agtatacata tcttacctcg atagtgttca tttcttccgt
     5941 cctaaatgct tgaggactgc agtctatcat gaaatcctaa ttggatattt agaatatgtc
     6001 aagaaattag gttacacaac agggcatatt tgggcatgtc caccaagtga gggagatgat
     6061 tatatcttcc attgccatcc tcctgaccag aagataccca agcccaagcg actgcaggaa
     6121 tggtacaaaa aaatgcttga caaggctgta tcagagcgta ttgtccatga ctacaaggat
     6181 atttttaaac aagctactga agatagatta acaagtgcaa aggaattgcc ttatttcgag
     6241 ggtgatttct ggcccaatgt tctggaagaa agcattaagg aactggaaca ggaggaagaa
     6301 gagagaaaac gagaggaaaa caccagcaat gaaagcacag atgtgaccaa gggagacagc
     6361 aaaaatgcta aaaagaagaa taataagaaa accagcaaaa ataagagcag cctgagtagg
     6421 ggcaacaaga agaaacccgg gatgcccaat gtatctaacg acctctcaca gaaactatat
     6481 gccaccatgg agaagcataa agaggtcttc tttgtgatcc gcctcattgc tggccctgct
     6541 gccaactccc tgcctcccat tgttgatcct gatcctctca tcccctgcga tctgatggat
     6601 ggtcgggatg cgtttctcac gctggcaagg gacaagcacc tggagttctc ttcactccga
     6661 agagcccagt ggtccaccat gtgcatgctg gtggagctgc acacgcagag ccaggacgtc
     6721 gacggctccg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
     6781 ggcccaatcg atatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     6841 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     6901 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     6961 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     7021 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     7081 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     7141 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     7201 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     7261 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     7321 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     7381 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     7441 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     7501 ctgtacaagt aatttaaaga attcaagctt cggggactag tcatatgata atcaacctct
     7561 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7621 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7681 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7741 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7801 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7861 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7921 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     7981 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     8041 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     8101 tcagacgagt cggatctccc tttgggccgc ctccccgcat cggtacaatt cactcctcag
     8161 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     8221 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     8281 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     8341 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     8401 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     8461 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     8521 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     8581 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     8641 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     8701 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8761 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8821 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     8881 ctcaattccg atcatattca ataaccctta atataacttc gtataatgta tgctatacga
     8941 agttattagg tctgaagagg agtttacgtc cagccaagct taggatcaat tctcatgttt
     9001 gacagcttat catcgataag ctgatcctca caggccgcac ccagcttttc ttccgttgcc
     9061 ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg aggggtcccg agaatcccca
     9121 tccctaccgt ccagcaaaaa gggggacgag gaatttgagg cctggcttga ggctcaggac
     9181 gcaaatcttg aggatgttca gcgggagttt tccgggctgc gagtaattgg tgatgaggac
     9241 gaggatggtt cggaggatgg ggaattttca gacctggatc tgtctgacag cgaccatgaa
     9301 ggggatgagg gtgggggggc tgttggaggg ggcaggagtc tgcactccct gtattcactg
     9361 agcgtcgtct aataaagatg tctattgatc tcttttagtg tgaatcatgt ctgacgaggg
     9421 gccaggtaca ggacctggaa atggcctagg agagaaggga gacacatctg gaccagaagg
     9481 ctccggcggc agtggacctc aaagaagagg gggtgataac catggacgag gacggggaag
     9541 aggacgagga cgaggaggcg gaagaccagg agccccgggc ggctcaggat cagggccaag
     9601 acatagagat ggtgtccgga gaccccaaaa acgtccaagt tgcattggct gcaaagggac
     9661 ccacggtgga acaggagcag gagcaggagc gggaggggca ggag
//
