LOCUS       sequence-198704-       10697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EBNA episome plasmid for CAG promoter constitutive expression of 
            dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. 
            Includes p53 shRNA expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: pCXLE-dCas9VPPH-T2A-GFP-shP53
FEATURES             Location/Qualifiers
     source          1..10697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..27
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             31..51
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(61..84,85..87,88..2643,2644..2646,2647..4161)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(61..84,85..87,88..2574,2575..2577,2578..4161)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4165..4185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4222..4332
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4384..4494
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4546..4656
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4708..4818
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4867..6717
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      6481..6490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7321..7692
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             7726..7779
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7786..7788,7789..7791,7792..8505)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7786..7788,7789..7791,7792..8502)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8092..8111)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8439..8460
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8543..9131
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8596..8616)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9014..9025)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9222..9241)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9287..9342
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9341..9360)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9703..9719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9703..9719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9716..9738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9727..9743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9751..9757,9758..9775,9776..9781))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9796..9817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    9906..9939
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tacccatacg atgttccaga ttacgcttcg ccgaagaaaa agcgcaaggt cgaagcgtcc
       61 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
      121 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
      181 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
      241 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
      301 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
      361 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
      421 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
      481 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
      541 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
      601 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
      661 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
      721 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg
      781 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
      841 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
      901 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
      961 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     1021 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     1081 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     1141 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     1201 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     1261 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     1321 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     1381 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     1441 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     1501 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     1561 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     1621 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     1681 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     1741 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     1801 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     1861 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     1921 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     1981 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     2041 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     2101 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     2161 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     2221 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     2281 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     2341 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     2401 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     2461 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     2521 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt cgacgccatc
     2581 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     2641 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     2701 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     2761 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     2821 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     2881 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     2941 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     3001 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     3061 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     3121 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     3181 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     3241 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     3301 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     3361 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     3421 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     3481 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     3541 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     3601 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     3661 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     3721 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     3781 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     3841 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     3901 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     3961 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     4021 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     4081 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     4141 ctgtctcagc tgggaggcga cagccccaag aagaagagaa aggtggaggc cagcctcgag
     4201 tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat gctcccagcc
     4261 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     4321 cttgacatgc tccccgggtc actacccagc gctagcgtcg agtgcgaaag cggcgggccg
     4381 gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
     4441 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
     4501 tcactaccca gcgctagcgt cgagtgcgaa agcggcgggc cggccgacgc ccttgacgat
     4561 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     4621 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtcactacc cagcgctagc
     4681 gtcgagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
     4741 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     4801 tttgaccttg acatgctccc cgggtcacta cccagcgcta gcgtcgagtt cgaaggcagc
     4861 ggcgggattt tcaaaccaga agaactacga caggcactga tgccaacttt ggaggcactt
     4921 taccgtcagg atccagaatc ccttcccttt cgtcaacctg tggaccctca gcttttagga
     4981 atccctgatt actttgatat tgtgaagagc cccatggatc tttctaccat taagaggaag
     5041 ttagacactg gacagtatca ggagccctgg cagtatgtcg atgatatttg gcttatgttc
     5101 aataatgcct ggttatataa ccggaaaaca tcacgggtat acaaatactg ctccaagctc
     5161 tctgaggtct ttgaacaaga aattgaccca gtgatgcaaa gccttggata ctgttgtggc
     5221 agaaagttgg agttctctcc acagacactg tgttgctacg gcaaacagtt gtgcacaata
     5281 cctcgtgatg ccacttatta cagttaccag aacaggtatc atttctgtga gaagtgtttc
     5341 aatgagatcc aaggggagag cgtttctttg ggggatgacc cttcccagcc tcaaactaca
     5401 ataaataaag aacaattttc caagagaaaa aatgacacac tggatcctga actgtttgtt
     5461 gaatgtacag agtgcggaag aaagatgcat cagatctgtg tccttcacca tgagatcatc
     5521 tggcctgctg gattcgtctg tgatggctgt ttaaagaaaa gtgcacgaac taggaaagaa
     5581 aataagtttt ctgctaaaag gttgccatct accagacttg gcacctttct agagaatcgt
     5641 gtgaatgact ttctgaggcg acagaatcac cctgagtcag gagaggtcac tgttagagta
     5701 gttcatgctt ctgacaaaac cgtggaagta aaaccaggca tgaaagcaag gtttgtggac
     5761 agtggagaga tggcagaatc ctttccatac cgaaccaaag ccctctttgc ctttgaagaa
     5821 attgatggtg ttgacctgtg cttctttggc atgcatgttc aagagtatgg ctctgactgc
     5881 cctccaccca accagaggag agtatacata tcttacctcg atagtgttca tttcttccgt
     5941 cctaaatgct tgaggactgc agtctatcat gaaatcctaa ttggatattt agaatatgtc
     6001 aagaaattag gttacacaac agggcatatt tgggcatgtc caccaagtga gggagatgat
     6061 tatatcttcc attgccatcc tcctgaccag aagataccca agcccaagcg actgcaggaa
     6121 tggtacaaaa aaatgcttga caaggctgta tcagagcgta ttgtccatga ctacaaggat
     6181 atttttaaac aagctactga agatagatta acaagtgcaa aggaattgcc ttatttcgag
     6241 ggtgatttct ggcccaatgt tctggaagaa agcattaagg aactggaaca ggaggaagaa
     6301 gagagaaaac gagaggaaaa caccagcaat gaaagcacag atgtgaccaa gggagacagc
     6361 aaaaatgcta aaaagaagaa taataagaaa accagcaaaa ataagagcag cctgagtagg
     6421 ggcaacaaga agaaacccgg gatgcccaat gtatctaacg acctctcaca gaaactatat
     6481 gccaccatgg agaagcataa agaggtcttc tttgtgatcc gcctcattgc tggccctgct
     6541 gccaactccc tgcctcccat tgttgatcct gatcctctca tcccctgcga tctgatggat
     6601 ggtcgggatg cgtttctcac gctggcaagg gacaagcacc tggagttctc ttcactccga
     6661 agagcccagt ggtccaccat gtgcatgctg gtggagctgc acacgcagag ccaggacgtc
     6721 gacactagcg tcgagttcga aggcagcggc gggccttcag ggcagatcag caaccaggcc
     6781 ctggctctgg cccctagctc cgctccagtg ctggcccaga ctatggtgcc ctctagtgct
     6841 atggtgcctc tggcccagcc acctgctcca gcccctgtgc tgaccccagg accaccccag
     6901 tcactgagcg ctccagtgcc caagtctaca caggccggcg aggggactct gagtgaagct
     6961 ctgctgcacc tgcagttcga cgctgatgag gacctgggag ctctgctggg gaacagcacc
     7021 gatcccggag tgttcacaga tctggcctcc gtggacaact ctgagtttca gcagctgctg
     7081 aatcagggcg tgtccatgtc tcatagtaca gccgaaccaa tgctgatgga gtaccccgaa
     7141 gccattaccc ggctggtgac cagcagccag cggccccccg accccgctcc aactcccctg
     7201 ggaaccagcg gcctgcctaa tgggctgtcc ggagatgaag acttctcaag catcgctgat
     7261 atggacttta gtgccctgct gtcacagatt tcctctagtg ggcagggagg aggtggaagc
     7321 ggcttcagcg tggacaccag tgccctgctg gacctgttca gcccctcggt gaccgtgccc
     7381 gacatgagcc tgcctgacct tgacagcagc ctggccagta tccaagagct cctgtctccc
     7441 caggagcccc ccaggcctcc cgaggcagag aacagcagcc cggattcagg gaagcagctg
     7501 gtgcactaca cagcgcagcc gctgttcctg ctggaccccg gctccgtgga caccgggagc
     7561 aacgacctgc cggtgctgtt tgagctggga gagggctcct acttctccga aggggacggc
     7621 ttcgccgagg accccaccat ctccctgctg acaggctcgg agcctcccaa agccaaggac
     7681 cccactgtct cctctagagt cgacactagc gtcgacggct ccggagaggg cagaggaagt
     7741 ctgctaacat gcggtgacgt cgaggagaat cctggcccaa tcgatatggt gagcaagggc
     7801 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     7861 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     7921 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     7981 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     8041 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     8101 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     8161 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     8221 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     8281 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     8341 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     8401 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     8461 accgccgccg ggatcactct cggcatggac gagctgtaca agtaatttaa agaattcaag
     8521 cttcggggac tagtcatatg ataatcaacc tctggattac aaaatttgtg aaagattgac
     8581 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     8641 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8701 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     8761 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8821 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8881 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     8941 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     9001 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     9061 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     9121 cgcctccccg catcggtaca attcactcct caggtgcagg ctgcctatca gaaggtggtg
     9181 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     9241 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     9301 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     9361 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     9421 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     9481 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     9541 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     9601 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     9661 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     9721 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     9781 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     9841 tttccagtcg ggaaacctgt cgtgccagcg gatctcaatt ccgatcatat tcaataaccc
     9901 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac
     9961 gtccagccaa gcttaggatc aattctcatg tttgacagct tatcatcgat aagctgatcc
    10021 tcacaggccg cacccagctt ttcttccgtt gccccagtag catctctgtc tggtgacctt
    10081 gaagaggaag aggaggggtc ccgagaatcc ccatccctac cgtccagcaa aaagggggac
    10141 gaggaatttg aggcctggct tgaggctcag gacgcaaatc ttgaggatgt tcagcgggag
    10201 ttttccgggc tgcgagtaat tggtgatgag gacgaggatg gttcggagga tggggaattt
    10261 tcagacctgg atctgtctga cagcgaccat gaaggggatg agggtggggg ggctgttgga
    10321 gggggcagga gtctgcactc cctgtattca ctgagcgtcg tctaataaag atgtctattg
    10381 atctctttta gtgtgaatca tgtctgacga ggggccaggt acaggacctg gaaatggcct
    10441 aggagagaag ggagacacat ctggaccaga aggctccggc ggcagtggac ctcaaagaag
    10501 agggggtgat aaccatggac gaggacgggg aagaggacga ggacgaggag gcggaagacc
    10561 aggagccccg ggcggctcag gatcagggcc aagacataga gatggtgtcc ggagacccca
    10621 aaaacgtcca agttgcattg gctgcaaagg gacccacggt ggaacaggag caggagcagg
    10681 agcgggaggg gcaggag
//
