LOCUS       sequence-198711-        9622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dominant negative glutamate receptor subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Robert A, Hyde R, Hughes TE, Howe JR
  TITLE     The expression of dominant-negative subunits selectively suppresses 
            neuronal AMPA and kainate receptors.
  JOURNAL   Neuroscience. 2002;115(4):1199-210.
  PUBMED    12453491
REFERENCE   2  (bases 1 to 9622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2002"; volume: "115(4)"; pages: "1199-210"
COMMENT     Sequence Label: pRH8
FEATURES             Location/Qualifiers
     source          1..9622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        47..426
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        427..630
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     581..601
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     675..694
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        675..693
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     745..761
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     746..762
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5406..5423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5412..5636
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(5768..6356)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5848..5867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6685..6732
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6757..6776
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6964..7758)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6964..7758)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7075..7094)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7685..7704
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7793..8150)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7807..7942
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7959..8150
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8107..8127
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8210..9001,9002..9070))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8833..8852
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9071..9175)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(9202..9622,1..35)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9289..9308)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9499..9520
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcgaatttt aacaaaatat taacgcttac aatttacgcg cgcgttgaca ttgattattg
       61 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      121 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      181 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      241 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      301 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      361 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      421 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      481 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      541 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      601 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
      661 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac
      721 ttaagcttgg taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat
      781 tcgggctcgc aagggcttcg ccaggctgga cattgtgctt gctggatttt tcccggatgc
      841 tcccggacta acatggatgt cccaccatcc cttgcagtgg aagcttgctc cttggcgcag
      901 tgagagtgaa gaacatgcag cgactgctaa tgggtttggg aagcggagac tccttcctct
      961 ttctgtgacc atgccgtgat tgtgtctgcg gccactactc cacgcatctt ccttctcgtc
     1021 caagcccgga gcctaacgct agatcgggga agtgggtgcc gcgcgcgcag gcacggaaac
     1081 atcatgaaga ttatttcccc agttttaagt aatctagtct tcagtcgctc cattaaagtc
     1141 ctgctctgct tattgtggat cggatattcg caaggaacca cacatgtgtt aagattcggt
     1201 ggtatatttg aatatgtgga atctggcccc atgggagcag aagaacttgc attcagattt
     1261 gctgtgaata ccatcaacag aaacaggact ttgctgccca acaccacttt aacttatgat
     1321 actcagaaga tcaatctcta tgacagtttt gaagcatcta agaaagcttg tgatcagctg
     1381 tctcttgggg tggctgctat cttcggtcct tcacacagtt catcagccaa tgctgtgcag
     1441 tccatctgca atgctctggg ggttccccac atacagaccc gctggaagca ccaggtgtca
     1501 gacaacaagg attccttcta cgtcagtctc tacccagact tctcttccct gagccgcgcc
     1561 atcttggatt tggtgcagtt ttttaagtgg aaaactgtca cagttgtgta tgacgacagc
     1621 actggtctca ttcgcttgca agagctcatc aaagctccat cgaggtacaa tcttcgactt
     1681 aaaattcgtc agctgccagc tgataccaaa gatgcaaaac ctttgctgaa ggagatgaaa
     1741 agaggcaagg agttccacgt gatcttcgac tgcagccatg agatggcagc aggcatttta
     1801 aaacaggcat tagctatggg aatgatgaca gaatactatc actatatatt tacaactctg
     1861 gacctctttg ctcttgacgt ggagccctac agatacagtg gcgtaaatat gacagggttc
     1921 aggatactaa atacagagaa tacccaagtc tcctccatca tcgaaaagtg gtctatggaa
     1981 cggttacagg cgcctccaaa acctgactca ggtttgctgg atggatttat gacgactgat
     2041 gctgctctga tgtatgatgc agtgcacgtt gtgtctgtgg ctgtccaaca gtttccccag
     2101 atgacagtca gctccttgca atgcaatcga cacaaaccct ggcgctttgg gacccgcttc
     2161 atgagtctaa ttaaagaggc tcactgggaa ggtctcacag gcagaataac atttaacaaa
     2221 accaatggat tacggacaga ttttgatttg gatgtgatca gtctcaagga agaaggtctg
     2281 gagaagattg gaacttggga tccagccagt ggcctgaata tgacagaaag tcagaaagga
     2341 aagccagcaa atatcacaga ctcattgtct aatcgttctt tgattgttac caccattttg
     2401 gaagaaccgt atgttctgtt taagaagtct gacaaaccac tctatgggaa tgatcgattt
     2461 gaaggctact gtattgatct cctacgagag ttatctacaa tccttggctt tacatatgag
     2521 attaggcttg tggaggatgg gaaatatgga gcccaggatg atgtgaacgg acaatggaat
     2581 ggaatggttc gtgaactaat cgatcataaa gctgaccttg cagttgctcc actggctata
     2641 acctatgttc gtgagaaggt catcgacttt tcaaagccgt ttatgacact tggaataagt
     2701 attttgtacc gcaagcccaa tggtacaaac ccaggcgtct tctccttcct gaatcctctc
     2761 tcccctgata tctggatgta tgttctgctg gcttgcttgg gtgtcagttg tgtgctcttt
     2821 gtcatagcca ggtttagtcc ctatgagtgg tataacccac acccttgcaa ccctgactca
     2881 gacgtggtgg aaaacaattt taccttgcta aatagtttct ggtttggagt tggagctctc
     2941 aggcggcaag gttctgagct catgcccaaa gcactctcca ccaggatagt gggaggcatt
     3001 tggtggtttt tcacacttat catcatttct tcgtataccg ctaacctagc cgcctttctg
     3061 actgtggaac gcatggagtc gcccattgac tctgctgacg atttagctaa gcaaaccaag
     3121 atagagtatg gagcagtgga ggacggcgca accatgacgt tttttaagaa atcaaaaatt
     3181 tcaacgtatg ataaaatgtg ggcgtttatg agcagcagga gacagtctgt gcttgtcaaa
     3241 agcaatgagg aagggatcca acgagtcctc acctcggatt atgctttctt aatggagtca
     3301 acaaccatcg agtttgttac acagcggaac tgtaacctca cgcagattgg cggccttata
     3361 gactccaaag gctatggcgt tggcactcct atgggctctc catatcgaga caaaatcacc
     3421 atagcaattc ttcagctgca ggaggaaggc aagctgcaca tgatgaagga gaaatggtgg
     3481 cggggcaatg gctgcccaga ggaggagagc aaagaggcca gtgctctggg ggtgcagaat
     3541 attggtggta tcttcattgt cctggcagcc ggcttggtgc tctcagtttt tgtggcagtg
     3601 ggagagtttt tatacaaatc caaaaaaaac gctcaattgg aaaagaggtc cttctgtagc
     3661 gctatggtgg aagagctgag aatgtccctg aagtgccagc gtcggctcaa acataagcca
     3721 caggccccag ttattgtgaa aacagaagaa gttatcaaca tgcacacatt taacgacaga
     3781 aggttgccag gtaaagaaac catggcatga agctgggagg ccaatcaccc aagcacaaac
     3841 tgtcgtcttt tttttttttt ttccaaacaa tttagcgaga atgtttcctg tggaaatatg
     3901 caacctgtgc aaaataaaat gagttacctc atgccgctgt gtctatgaac tagagactct
     3961 gtgatctaag cagtttcagt gatcagactt gatttacaag caccgtggat caaccaagtt
     4021 acacggggtt acactgttta tcataggttc ctcccttcct ttgagtgaat gttacatgca
     4081 aatgttgtgg ctggtttcaa atgcagtcca gggagaaact gctggttcct tcggaagctc
     4141 agctgtcgtc aggagatgga atgccggtgc ccaaaagggt aaccaataaa aatgccataa
     4201 aaattttaaa aaaatgcgtg agatcggcaa aaattatagt gttacaagaa acagtacagt
     4261 cccatggtca ccaacacaat agaggtgata atgttactag cccccaatac tcagtaaaat
     4321 cgtcatctga atagataata tgtgttcata gaatgtgaaa aaaaatgtaa tgcgagacac
     4381 accagtatca atagaagtgg aactgaaggc agaacatcat cagttacttt tctttttcaa
     4441 tagtctgtgt catggattgt gatatagatg gcaattatca agccaataat tttttttctg
     4501 aaaataccta tggcaaatat tttaataggc aacttgctcc cacaaatccc tactctaacc
     4561 tcccccagaa atataaaagg aaccattggt ttagagattg gtatgtaaga gatgatgttt
     4621 tgcaagcctt gtcgtgcatt gtaaaagggc tcagtgttac tggttacagg gaagactgaa
     4681 gctttcaccc tgacattctg aaatgtcaac cgaaactctc cttcctcctg taaaggacct
     4741 tgatggggca gattccattg atcaaagaat ggggacttgt cacctataca atggtacgtg
     4801 acagaacttt gaggtggact gcatttaata atagtcacaa tgttaaaaga acaaaattct
     4861 tgagcagttt tttttttttg ttttgttttg ttttcaaaaa atgttcaggt ttatttgtgg
     4921 aaatgcaaga tttctatgaa aatagttttt gtatggaaat ttttgtaata ctttttatca
     4981 acaaaataag aacacatgtt tctgtcaggg gtgtgaggtc aagcatgaac ggtagtgcgt
     5041 gtgcaccacc aacgtttggt gaaaactatt tttatcaaga aaaaggaatc atagaagaga
     5101 aatattttca agttagatac tataaaagct aggtgcacta ccaccacggc ttgtcgcgcc
     5161 acacccctga gtcccacaag gtggataaca tattgtaatg aacagttgtg tgtaaaatgg
     5221 caaaagacac agacctcttg acaacattgt gaaaacagtt gagtgcacac agtttgctgt
     5281 ttgaatccaa tgcacaaaaa ttttacaaaa atccattaaa attatgtccg ttttaaaacc
     5341 tgcagcccat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg
     5401 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5461 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5521 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5581 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5641 tgaggcggaa agaaccagtg gcggtaatac ggttatccac agaatcaggg gataacgcag
     5701 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5761 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5821 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5881 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5941 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6001 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6061 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6121 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6181 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6241 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6301 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6361 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6421 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6481 gttttaaatc aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc
     6541 cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg
     6601 aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc
     6661 cctgggaccg aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc
     6721 tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta
     6781 gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     6841 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     6901 cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
     6961 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     7021 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     7081 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     7141 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     7201 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
     7261 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     7321 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     7381 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     7441 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     7501 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     7561 ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     7621 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     7681 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     7741 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa
     7801 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     7861 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     7921 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     7981 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     8041 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     8101 ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag gactcttcct
     8161 ttttcaataa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8221 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8281 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8341 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8401 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8461 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8521 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8581 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8641 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8701 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8761 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8821 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8881 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8941 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9001 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9061 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9181 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9241 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9301 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9361 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9421 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9481 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9541 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9601 agctgattta acaaaaattt aa
//
