LOCUS       sequence-198713-       10096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synOptopatch construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE
  TITLE     All-optical synaptic electrophysiology probes mechanism of 
            ketamine-induced disinhibition.
  JOURNAL   Nat Methods. 2018 Oct;15(10):823-831. doi: 
            10.1038/s41592-018-0142-8. Epub 2018 Oct 1.
  PUBMED    30275587
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0142-8"; journalName: "Nat Methods"; date: 
            "2018-10"; volume: "15"; issue: "10"; pages: "823-831"
COMMENT     Sequence Label: LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark...
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..660
                     /label=chicken beta-actin promoter
     intron          661..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1729..1740
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             1816..1833
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1837..1869
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1873..1881,1882..1896)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1924..1980
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     2676..2695
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4858..5199
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             5215..5271
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5272..6030
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAARERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             join(6100..6102,6103..6813)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGGGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6142..6163)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6403..6422)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6750..6771
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7118..7137)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7183..7238
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7237..7256)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7599..7615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7599..7615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7612..7634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7623..7639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7647..7653,7654..7671,7672..7677))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7692..7713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7772..7967
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7818..7953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7973..8107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8010..8029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8064..8083
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8175..8192)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8346..8934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8426..8445)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9105..9896,9897..9965))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9728..9747
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9966..10070)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc
      841 cttaaagggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgt catcctcatc
     1741 gccgccacca tgctgcagaa cgagcttgct cttaagttgg ctggacttga tattaacaag
     1801 actggaggag gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
     1861 caaatgggtc gggatctgta cgacgatgac gataaggatc tcgcaacaat ggtcgactca
     1921 tcacgtcgta agtggaataa ggcaggtcac gcagtcagag ctataggtcg gctgagctca
     1981 cccgtggttt ccgagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag
     2041 gggctgaggc tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc
     2101 aagaagcccg tgcagctgcc cggcgcctac atcgtggaca tcaagttgga catcgtgtcc
     2161 cacaacgagg actacaccat cgtggaacag tgcgaacgcg ccgagggccg ccactccacc
     2221 ggcggcatgg acgagctgta caagggaggt acaggcggga gtctggtgag caagggcgag
     2281 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2341 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt
     2401 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2461 tcccctcagt tcatgtacgg ctccaaggcc tacattaagc acccagccga catccccgac
     2521 tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac
     2581 ggcggcatta ttcacgtgaa ccaggactcc tccctgcagg acggcgtatt catctacaag
     2641 gtgaagctgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     2701 ggctgggagg ctacgcgtga cgacctgact gaagagcaga tcgcagaatt taaagaggct
     2761 ttctccctat ttgacaagga cggggatggg acgataacaa ccaaggagct tgggacggtg
     2821 ttccgatcgc tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta
     2881 gatgccgacg gtgacggcac attcgacttc cctgagttcc tgacgatgat ggcaagaaaa
     2941 atgaatgaca cagacagtga agaggaaatt cgcgaagcgt tccgcgtgtt tgataaggac
     3001 ggcaatggct acatcggcgc agcagagctt cgccacgtga tgacagacct tggagagaag
     3061 ttaacagatg aggaggttga tgaaatgatc agggtagcag acatcgatgg ggatggtcag
     3121 gtaaactacg aagagtttgt acaaatgatg acagcgaagg gtggaggttc tggtggaggt
     3181 tctggaggtg gatcaggagg aggttcgaga tctaagctct caggtggcgg agggagcggc
     3241 ggaggtggct ctggaggtgg tggctcagct gaagcatggt acaatctggg aaacgcttat
     3301 tacaagcagg gcgactatca aaaagccatt gagtattacc aaaaggctct tgagctagac
     3361 cctaataacg ccgaagcgtg gtataaccta ggcaacgcat attacaagca gggagactac
     3421 caaaaggcaa tcgaatatta ccagaaggca ctcgaactgg acccaaataa cgcagaggct
     3481 tggtataatc tgggaaacgc ctactataaa cagggtgact accaaaaggc gatagaggac
     3541 tatcagaagg ccttggagct cgatccgaac aatctgcaac gtagtgctgg tggcggaggg
     3601 tctggtggag gaggctcagg aggaggtggc gcatcaaacg ttgccaagta cgggcacctt
     3661 gaagccagcc ctgatgatgg gcagaatcag ttcagtgata tcattaagtc cagatctgat
     3721 gaacacaacg atgtacagaa gaaaaccttt accaaatgga taaacgctcg attttcaaag
     3781 agtgggaaac cacccatcaa tgatatgttc tcagacctca aagatgggag aaagctcttg
     3841 gatcttctag aaggcctcac aggaacatca ttgccaaagg aacgtggttc cacaagggtg
     3901 catgccttaa ataatgtcaa ccgggtgctg caggttttac atcagaacaa tgtggagctg
     3961 gtgaatatcg gaggcactga cattgtggat ggaaatccca agctgacgct gggtttgctg
     4021 tggagtatta ttctgcactg gcaggtgaag gatgtcatga aagatatcat gtcagacctg
     4081 cagcagacga acagcgagaa gatcctgctg agctgggtac ggcagaccac caggccctac
     4141 agccaagtca acgtcctcaa cttcaccacc agctggacag atggactcgc attcaacgcc
     4201 gtgctccacc ggcacaaacc agatctcttc agctgggaca gagtggtcaa aatgtcccca
     4261 actgagagac ttgaacatgc ttttagcaag gcccatactt atttggggat tgaaaaactt
     4321 ctggatcctg aagatgttgc cgtgcagctc cctgacaaga aatccataat tatgtattta
     4381 acgtctctgt ttgaggtgct tcctcagcaa gtcactatag accaattgaa gctctcaggt
     4441 ggcggaggga gcggcggagg tggctctgga ggtggtggct cagctgaagc atggtacaat
     4501 ctgggaaacg cttattacaa gcagggcgac tatcaaaaag ccattgagta ttaccaaaag
     4561 gctcttgagc tagaccctaa taacgccgaa gcgtggtata acctaggcaa cgcatattac
     4621 aagcagggag actaccaaaa ggcaatcgaa tattaccaga aggcactcga actggaccca
     4681 aataacgcag aggcttggta taatctggga aacgcctact ataaacaggg tgactaccaa
     4741 aaggcgatag aggactatca gaaggccttg gagctcgatc cgaacaatct gcaacgtagt
     4801 gctggtggcg gagggtctgg tggaggaggc tcaggaggag gtggcgcatc aaacgaattc
     4861 cagtgccgga tctgcatgcg gaacttcagc gaccggtcca acctgagcag gcacatcaga
     4921 acccacaccg gagaaaagcc cttcgcctgc gacatttgcg gccggaagtt cgccatcagc
     4981 agcaacctga acagccacac caagatccac actggcagcc agaaaccttt ccagtgcaga
     5041 atttgtatga gaaactttag cagaagcgac aacctggcca gacacatccg gacacatact
     5101 ggtgaaaaac cttttgcctg tgatatctgt ggcagaaagt ttgccacctc cggcaatctg
     5161 acccggcaca caaagattca cctgcggggc agccagctat cgattggctc cggagccacg
     5221 aacttctctc tgttaaagca agcaggagac gtggaagaaa accccggtcc catggtaagt
     5281 atcgctctgc aggctggtta cgacctactg ggtgacggca gacctgaaac cctgtggctg
     5341 ggcatcggca ctctgctgat gctgattgga accttctact ttctggtccg cggatgggga
     5401 gtcaccgata aggatgcccg ggaatattac gctgtgacta tcctggtgtc cggaatcgca
     5461 tccgccgcat atctgtctat gttctttggt atcgggctta ctgaggtgtc cgtcgggggc
     5521 gaaatgttgg atatctatta tgccaggtac gcccagtggc tgtttaccac cccacttctg
     5581 ctgctgcacc tggcccttct cgctaaggtg gatcgggtga ccatcggcac cctggtgggt
     5641 gtggacgccc tgatgatcgt cactggcctc atcggagcct tgagccacac ggccatagcc
     5701 agatacagtt ggtggttgtt ctctacaatt tgcatgatag tggtgctcta tgttctggct
     5761 acatccctgc gatctgctgc aagggagcgg ggccccgagg tggcatctac ctttaacacc
     5821 ctgacagctc tggtcttggt gctgtggacc gcttacccta tcctgtggat cataggcact
     5881 gagggcgctg gcgtggtggg cctgggcatc gaaactctgc tgtttatggt gttggacgtg
     5941 actgccaagg tcggctttgg ctttatcctg ttgagatccc gggctattct gggcgacacc
     6001 gaggcaccag aacccagtgc cggtgccgat acgttgatga agagtagaat cacaagcgaa
     6061 ggcgagtaca tccccctgga tcaaatagac ataaatgtag tgagcaaggg cgaggagctg
     6121 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6181 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     6241 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggcggcggc
     6301 ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6361 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     6421 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6481 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6541 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     6601 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6661 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     6721 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6781 gggatcactc tcggcatgga cgagctgtac aaatcaagga tcacctctga gggcgagtat
     6841 atccctctgg atcagatcga cattaacgtc ggtggaaaat cccgcataac ctctgagggc
     6901 gagtacatcc ctctggatca aatcgatata aacgtggggg gaaaaagtcg aatcacttct
     6961 gagggcgagt atatacccct cgatcagatt gacatcaatg tggggggctt ttgttatgag
     7021 aatgaagtgt aggcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     7081 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     7141 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     7201 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     7261 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     7321 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     7381 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     7441 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     7501 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     7561 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     7621 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7681 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7741 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     7801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7921 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt
     7981 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8041 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8101 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8161 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8221 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8281 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8461 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8521 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8581 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8641 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8701 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8761 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8821 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8881 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8941 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9001 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9061 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9121 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9181 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9241 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9301 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9361 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9421 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9481 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9541 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9601 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9661 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9721 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9781 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9841 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9901 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9961 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10021 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10081 cgaaaagtgc cacctg
//
