LOCUS       sequence-198714-        3510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express DNA binding domain of human Zinc finger protein 57 in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Zuo Z, Roy B, Chang YK, Granas D, Stormo GD
  TITLE     Measuring quantitative effects of methylation on transcription 
            factor-DNA binding affinity.
  JOURNAL   Sci Adv. 2017 Nov 17;3(11):eaao1799. doi: 10.1126/sciadv.aao1799. 
            eCollection 2017 Nov.
  PUBMED    29159284
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2017-11-17"; volume: "3(11):eaao1799. doi"; pages: " 
            10.1126/sciadv.aao1799. eCollection 2017 Nov"
COMMENT     Sequence Label: hisSUMO-ZFP57
FEATURES             Location/Qualifiers
     source          1..3510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             95..112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             131..421
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
     CDS             422..439
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     terminator      1506..1553
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1771..2359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1851..1870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2531..3322,3323..3391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3154..3173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3392..3496)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagtgctagt ggtgctagcc ccgcgaaatt aatacgactc actatagggt ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgcatcac catcaccatc acggttcttc
      121 tatggctagc atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga
      181 agtcaagcct gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt
      241 caagatcaaa aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg
      301 taaggaaatg gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac
      361 ccctgaagat ttggacatgg aggataacga tattattgag gctcacagag aacagattgg
      421 tctggtgcca cgcggtagta cccgtgtgtt gcaagcgtca caagctggcc cacccttctt
      481 ttgctatacg tgcggcaagt gtttcagtcg tcgtagctat ttgtactccc accagtttgt
      541 tcacaacccg aaattgacca actcatgcag ccagtgcggc aagctgttcc gttctccgaa
      601 aagtttaagt taccatcgcc gcatgcattt gggtgaacgt ccgttttgct gcacactgtg
      661 tgataaaacg tactgtgatg cctccggcct cagtcgccac cgccgcgtgc acctgggtta
      721 tcgcccacac tcttgctctg tttgtggaaa atccttccgt gaccaatccg aactgaaacg
      781 ccatcagaaa attcatcaaa atcaggaacc ggttgatgga aatcaggaat gcaccctccg
      841 tattccgggg acgcaggcag agtttcaaac gccgattgca cgttcgcaac gcagcattca
      901 gggcctgctg gatgttaacc atgccccagt cgcgcgttcg caggaaccga ttttccgcac
      961 agagggcccg atggcccaga accaggcctc cgtcctgaaa aaccaggcgc cggttacgcg
     1021 tacccaagcc ccaatcacag gcactctgtg tcaggatgcg cgctcaaaca gccatccggt
     1081 gaagccgtct cgcttaaacg tgttctgttg cccgcactgt agcttaacct tcagtaaaaa
     1141 aagttatctg tcccgccatc agaaagcaca tctgacggag ccgccgaatt attgttttca
     1201 ttgtagtaaa agtttcagct cgttttcgcg tttggtacgc catcagcaga cgcactggaa
     1261 gcaaaaatcc tacctgtgcc cgatctgcga tctgagtttt ggtgaaaaag aaggcctgat
     1321 ggaccattgg cgcggttata aaggaaaagg ttaatgagga tcccgggaat tctcgagtaa
     1381 ggttaacctg caggaggcct ttaattaagg tggtgcggcc gcgctagcgg tcccggggga
     1441 tcgatccggc tgctaacaaa gcccgaaagg aagctgagtt agctgctgcc accgctgaac
     1501 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     1561 gaggaactat atccggaagc ttggcactgg ccgaccgggg tcgagcactg actcgctgcg
     1621 ctcggtcgtt cggccgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1681 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1741 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1801 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1861 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1921 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1981 gtatctcagt tcggtgtggg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2041 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cgctaagaca
     2101 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2161 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2221 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2281 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2341 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2401 gaacgaaaac tcacagatcc aggattttgg tcatgagatt atcaaaaagg atcttcacct
     2461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2581 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2821 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2941 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3121 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3481 aaataggggt tccgcgcaca tttccccgaa
//
