LOCUS       sequence-198718-        4200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF436 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF436
FEATURES             Location/Qualifiers
     source          1..4200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1511..1610)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1627..1646)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1628..1646)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1651..1667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1651..1667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2098..2889
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2982..3570
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3471..3490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3724..3741
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3900..3927)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4019..4105)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4154..4176
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4168..4185
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4169..4185
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggcagccac cctgctcatg
      121 gctgggtccc aggcacctgt gacgtttgaa gatatggcca tgtatctcac ccgggaagaa
      181 tggagacctc tggacgctgc acagagggac ctttaccggg atgttatgca ggagaattat
      241 ggaaatgttg tctcactaga ttttgagatc aggagtgaga acgaggtaaa tcccaagcaa
      301 gagattagtg aagatgtaca atttgggact acatctgaaa gacctgctga gaatgctgag
      361 gaaaatcctg aaagtgaaga gggctttgaa agcggagata ggtcagaaag acaatgggga
      421 gatttaacag cagaagagtg ggtaagctat cctctccaac cagtcactga tctacttgtc
      481 cacaaagaag tccacacagg catccgctat catatatgtt ctcattgtgg aaaggccttc
      541 agtcagatct cagaccttaa tcgacatcag aagacccaca ctggagacag accctataaa
      601 tgttatgaat gtggaaaagg cttcagtcgc agctcacacc ttattcagca tcaaagaaca
      661 catactgggg agaggcctta tgactgtaac gagtgtggga aaagttttgg aagaagttct
      721 cacctgattc agcatcagac aatccacact ggagagaagc ctcacaaatg taatgagtgt
      781 ggaaaaagtt tctgccgtct ctctcaccta atccaacacc aaaggaccca cagtggtgag
      841 aaaccctatg agtgtgagga gtgtgggaaa agcttcagcc ggagctctca cctagctcag
      901 caccagagga cccacacggg tgagaaacct tatgaatgta acgaatgtgg ccgaggcttc
      961 agtgagagat ctgatctcat caaacactat cgagtccaca caggggagag gccctacaag
     1021 tgtgatgagt gtgggaagaa cttcagtcag aactccgacc ttgtgcgtca tcgcagagcc
     1081 cacacgggag agaagccata ccactgtaac gaatgtgggg aaaatttcag ccgcatctca
     1141 cacttggttc agcaccagag aactcacact ggagagaagc catatgaatg caatgcttgt
     1201 gggaaaagct tcagccggag ctctcatctc atcacacacc agaaaattca cactggagag
     1261 aagccttatg agtgtaatga gtgttggcga agctttggtg aaaggtcaga tctaattaaa
     1321 catcagagaa cccacacagg ggagaagccc tacgagtgtg tgcagtgtgg gaaaggtttc
     1381 acccagagct ccaacctcat cacacatcaa agagttcaca cgggagagaa accttatgaa
     1441 tgtaccgaat gtgagaagag tttcagcagg agctcagctc ttattaaaca taagagagtt
     1501 catacggact acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1561 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1621 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1681 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     1741 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     1801 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     1861 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1921 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     1981 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     2041 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     2101 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     2161 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     2221 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     2281 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     2341 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2401 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     2461 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2521 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2581 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2641 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2701 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2761 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2821 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     2881 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     2941 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3001 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3061 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3121 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3361 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3601 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3661 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3721 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3781 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3841 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3901 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3961 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4021 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4081 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4141 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
