LOCUS       sequence-19873-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Soeno Y, Abe H, Kimura S, Maruyama K, Obinata T
  TITLE     Generation of functional beta-actinin (CapZ) in an E. coli 
            expression system.
  JOURNAL   J Muscle Res Cell Motil. 1998 Aug . 19(6):639-46.
  PUBMED    9742448
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Muscle 
            Res Cell Motil. 1998 Aug . 19(6):639-46."
COMMENT     Sequence Label: CAP Z alpha 1 beta 1 CP
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             100..122
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cggcccgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact ttaagaagga
      121 gatatacata tgagtgacca gcagctggat tgtgccttgg atttgatgag gcgtctgcct
      181 ccgcagcaga tagagaagaa tctcagtgac ctcattgact tggtcccaag cctctgtgaa
      241 gatctcctct cctctgttga tcagccattg aagattgcac gagataaggt ggtaggaaaa
      301 gactatctat tgtgtgacta caacagagat ggagactcgt atagatcacc gtggagtaac
      361 aagtatgatc ctcccctgga agacggtgcc atgccatctg ctcgcctgcg caagctggag
      421 gtggaagcca acaatgcctt tgaccagtat agagacttgt atttcgaagg tggagtctcc
      481 tctgtctacc tctgggatct ggatcatggt tttgccggtg tgatcctcat taagaaagct
      541 ggagatggat caaagaagat taagggatgc tgggattcca tccacgtggt ggaggttcag
      601 gagaaatcca gcggccgtac tgctcattac aagctgacct ccacagtgat gctgtggctg
      661 cagactaata aaactggttc tggtaccatg aaccttggcg ggagcctcac cagacagatg
      721 gagaaagatg agactgtgag cgactcttct ccgcacatag ccaatattgg acgcttggt
//
