LOCUS       sequence-198740-        6645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF423 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF423
FEATURES             Location/Qualifiers
     source          1..6645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3988..4087)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4104..4123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4105..4123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4128..4144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4128..4144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4575..5366
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5459..6047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5948..5967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6201..6218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6377..6404)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6496..6582)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(6631..6645,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6645,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagttgg caccatgcat aagaagaggg ttgaagaggg ggaggcctca gacttctcgc
      181 tggcctggga ttcctccgtg acagcagcag gaggcctaga aggagagcca gagtgcgatc
      241 agaaaaccag ccgtgcgctg gaagacagga acagcgtgac aagtcaagag gagagaaatg
      301 aggatgatga agacatggag gatgaatcaa tttacacctg cgatcactgt cagcaggact
      361 tcgagtctct ggcagacctg acggaccacc gggcccaccg ctgtcctgga gatggtgatg
      421 acgacccaca actctcctgg gtggcctcgt ctccctccag caaggatgtt gcgtcaccca
      481 cgcagatgat cggagatggt tgtgacctcg gcctcggcga ggaggaaggg ggcacgggcc
      541 tgccataccc ttgccagttc tgcgacaagt ccttcatccg cttgagctac ttgaagaggc
      601 acgagcagat ccacagcgac aagctgccgt tcaagtgcac ctactgcagc cgcctcttca
      661 agcacaagag gagccgcgac cggcacatca agctgcatac gggcgacaag aagtatcact
      721 gccacgagtg cgaggcagcc ttctcccgca gcgaccacct caagatccac ctgaagaccc
      781 acagctccag caagcccttc aagtgcactg tgtgcaagcg cggcttctcc tccaccagct
      841 cgctgcagag ccacatgcag gcccacaaaa agaacaagga gcatctggcc aagtcggaga
      901 aggaagccaa gaaggacgac ttcatgtgcg actactgcga ggacaccttc agccagacgg
      961 aggagctgga gaagcacgtg ctcacccgcc acccgcagct gtccgagaag gcggacctgc
     1021 agtgcattca ctgccctgag gtcttcgtcg acgagaacac actgctcgcc catatccacc
     1081 aagcccacgc caaccagaaa cacaagtgcc ccatgtgccc tgagcagttc tcctcagtgg
     1141 aaggtgtcta ctgccacctg gacagccacc ggcagcccga ctccagcaac cacagtgtca
     1201 gtcccgaccc tgtactgggc agcgtggcct ccatgagcag cgccacaccc gactccagcg
     1261 cctctgtgga gcgtggctcc accccggact ccaccttgaa gccgctgcgg gggcagaaga
     1321 agatgcggga tgacgggcag ggctggacca aggtggtcta tagctgcccc tattgttcca
     1381 agcgggactt taacagcctg gccgtgctgg agatccacct gaagaccatc cacgcggaca
     1441 agccccagca gagccacaca tgtcagatct gcctggactc catgcccacc ctctacaacc
     1501 tcaacgagca cgttcgcaag ctgcacaaga accatgccta ccctgtgatg cagtttggca
     1561 acatctctgc cttccactgc aactactgcc ccgagatgtt cgccgacatc aatagcctgc
     1621 aggagcacat ccgcgtctcc cactgcggcc ccaacgccaa cccctctgac ggtaataatg
     1681 ctttcttctg caaccagtgc tccatgggtt tccttactga gtcctccctc accgagcaca
     1741 tccagcaggc ccactgcagt gtgggcagtg ccaaactaga gtctccggtg gtgcagccca
     1801 cgcagtcctt catggaggtc tattcctgcc cctactgcac caactccccc atctttggct
     1861 ccatcctgaa actcaccaag cacatcaagg agaaccacaa gaacattcca ctggcccaca
     1921 gcaagaagtc caaggccgag cagagcccag tctcgtccga tgtggaggtg tcttccccga
     1981 agcggcagcg gctctcagca agcgccaact ccatctccaa tggggagtat ccttgtaatc
     2041 aatgcgacct caagttctcc aactttgaga gcttccagac ccacctgaag ctgcacctgg
     2101 agctgctgct gcggaagcaa gcgtgccccc agtgcaaaga ggactttgac tcccaggagt
     2161 ccctcctgca gcacctgaca gtgcattaca tgaccacgtc gacccactat gtgtgcgaga
     2221 gctgcgacaa gcaattttcc tcggtggatg acctgcagaa gcacctgctg gacatgcaca
     2281 cctttgtgtt gtaccactgc accctgtgtc aggaggtctt cgactccaag gtgtccatcc
     2341 aggtgcacct ggcggtgaag cacagcaatg agaagaagat gtaccgctgc acggcctgca
     2401 actgggactt ccgcaaggag gctgacctgc aggtgcacgt caaacacagc cacctgggca
     2461 acccggccaa ggctcacaag tgcatcttct gtggggagac cttcagcacc gaggtggagc
     2521 tgcagtgcca catcaccaca cacagcaaga agtataactg taagttctgc agcaaggcct
     2581 tccacgccat catcctgctg gagaagcacc tgcgggagaa gcactgtgtg tttgatgctg
     2641 cgaccgagaa cggcacggcc aatggggtac ccccaatggc caccaagaaa gctgagcctg
     2701 ctgacctgca gggcatgctg cttaagaacc ctgaggcacc taacagccat gaggccagcg
     2761 aggatgacgt ggacgcgtcg gagcccatgt acggctgtga catctgcggg gcggcctaca
     2821 ccatggaggt gctgctgcag aatcaccggc tgcgggacca caatatccgg ccgggcgagg
     2881 atgatggctc acgcaagaag gctgagttta tcaagggcag tcacaagtgc aacgtttgtt
     2941 cacggacttt cttctcggag aacgggctac gggagcacct gcagacgcac cggggccctg
     3001 ccaagcacta catgtgtccc atctgtggtg agcgcttccc ttcgctgctg acgctcaccg
     3061 aacacaaggt gacccacagc aagagcctgg acacgggcac ctgtcgcatc tgcaagatgc
     3121 ccctgcagag cgaggaggag tttattgagc actgccagat gcaccctgac ctgcgcaact
     3181 cactcacggg cttccgctgt gtggtctgca tgcagacagt cacttccacg cttgagctca
     3241 agatccatgg caccttccac atgcagaagc tggcgggcag ctcagcggcg tcctccccca
     3301 atggccaggg gctgcagaag ctctacaagt gcgccctgtg cctcaaggag ttccgcagca
     3361 agcaggacct ggtgaagctt gacgtcaatg ggctgcccta cggcctctgc gccggctgca
     3421 tggcccgcag cgccaacgga caggtgggtg gcctggcccc gcccgagccc gccgaccggc
     3481 cctgtgccgg cctccgttgc cccgagtgca gtgtcaagtt tgagagtgcc gaagacctgg
     3541 agagccacat gcaggtggac caccgtgacc tcacgccgga gaccagtggg ccccggaaag
     3601 gcacccagac atcgccagtg ccccggaaaa agacatacca gtgcatcaag tgccagatga
     3661 ccttcgagaa cgagagagag atccaaatcc acgttgccaa ccacatgatt gaggaaggca
     3721 tcaaccacga gtgtaagctg tgcaaccaga tgttcgactc cccggccaag ctcctctgtc
     3781 acctcattga gcacagcttc gagggcatgg gcggcacctt caaatgcccc gtgtgtttca
     3841 cagtcttcgt ccaggccaac aagttgcagc agcacatctt tgccgtgcac gggcaggagg
     3901 acaagatcta cgactgctca cagtgccctc agaagttctt cttccagacc gagctgcaga
     3961 accacacgat gagccagcac gcacagttgc caactttctt gtacaaagtt ggcattataa
     4021 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     4081 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     4141 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     4201 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     4261 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     4321 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     4381 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     4441 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     4501 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     4561 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     4621 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     4681 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     4741 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     4801 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     4861 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     4921 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     4981 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     5041 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     5101 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     5161 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     5221 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     5281 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     5341 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     5401 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5461 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5521 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5581 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5641 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5701 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5761 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5821 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5881 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5941 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6001 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6061 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6301 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     6361 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     6421 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     6481 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     6541 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     6601 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgtt
//
