LOCUS       sequence-19875-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Bhattacharya N, Ghosh S, Sept D, Cooper JA
  TITLE     Binding of myotrophin/V-1 to actin-capping protein: implications for
            how capping protein binds to the filament barbed end.
  JOURNAL   J Biol Chem. 2006 Oct 13. 281(41):31021-30.
  PUBMED    16895918
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2006 Oct 13. 281(41):31021-30."
COMMENT     Sequence Label: actin CP alpha 2 beta 2
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    81..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             120..142
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggtgang ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
      121 ttgtttaact ttaagaagga gatatacata tgagcgatca gcagctggac tgcgccttgg
      181 acctgatgag gcgcctgcct ccacagcaga ttgagaagaa cctcagcgat ctgatcgacc
      241 tggtccccag tctgtgtgaa gatctcctgt catctgttga ccagcccctg aaaattgcca
      301 gagacaaggt ggtgggcaag gattaccttt tgtgtgacta caacagagac ggggactcct
      361 ataggtcacc gtggagtaac aagtatgacc ctcctttgga agatggggcc atgccatctg
      421 ctcggctcag aaagctggag gtagaggcca acaatgcctt cgaccaatac cgagacctgt
      481 attttgaagg tggggtctca tcagtctacc tctgggatct tgatcatggc tttgctggag
      541 tgatcctcat aaagaaagct ggagatggat ccaagaagat caaaggctgc tgggattcca
      601 tccacgtggt ggaagtgcag gagaagtcca gcggccgtac tgcccattac aagttgacct
      661 ccacggtgat gctatggctg caaaccaaca aatccggctc gng
//
