LOCUS       sequence-19876-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pDW1775
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             133..153
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             154..180
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             203..790
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 tcaaatgctc actgacgttc cataaattcc cctcggtatc caattagaaa ggtgggactc
       61 acgaagatct aactcgagaa agatattgta tatatcgtaa caataggagg ttcaacaatg
      121 gcttcctccc ctccaaagaa aaagagaaag gttagttgga aggacgcaag tggttggtct
      181 agacccggga accggtggat cccagctagt gaaatctgaa ttggaagaga agaaatctga
      241 acttagacat aaattgaaat atgtgccaca tgaatatatt gaattgattg aaatcgcaag
      301 aaattcaact caggatagaa tccttgaaat gaaggtgatg gagttcttta tgaaggttta
      361 tggttatcgt ggtaaacatt tgggtggatc aaggaaacca gacggagcaa tttatactgt
      421 cggatctcct attgattacg gtgtgatcgt tgatactaag gcatattcag gaggttataa
      481 tcttccaatt ggtcaagcag atgaaatgca aagatatgtc gaagagaatc aaacaagaaa
      541 caagcatatc aaccctaatg aatggtggaa agtctatcca tcttcagtaa cagaatttaa
      601 gttcttgttt gtgagtggtc atttcaaagg aaactacaaa gctcagctta caagattgaa
      661 tcatatcact aattgtaatg gagctgttct tagtgtagaa gagcttttga ttggtggaga
      721 aatgattaaa gctggtacat tgacacttga ggaagtgaga aggaaattta ataacggtga
      781 gataaacttt taataggagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
      841 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
      901 acgttaagca tgtaataatt aacatgtaat gcatgacgtt attta
//
