LOCUS       sequence-198777-       13347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13347)
  AUTHORS   Jiang G, Sancar A
  TITLE     Recruitment of DNA damage checkpoint proteins to damage in 
            transcribed and nontranscribed sequences.
  JOURNAL   Mol Cell Biol. 2006 Jan . 26(1):39-49.
  PUBMED    16354678
REFERENCE   2  (bases 1 to 13347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2006 Jan . 26(1):39-49."
COMMENT     Sequence Label: pcDNA3-ATR Kinase dead
FEATURES             Location/Qualifiers
     source          1..13347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(29..617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(109..128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(788..1579,1580..1648))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1411..1430
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1649..1753)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1828..1847)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2019..2398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2399..2602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2553..2573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2647..2666
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2647..2665
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2725..2748
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(10722..10739)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10728..10952
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10998..11426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11085..11104)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11295..11316
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11435..11455)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11440..11769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11620..11755
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11682..11701
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             11836..12630
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11890..11909)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12500..12519
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    12804..12925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12841..12860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12895..12914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12974..12990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12974..12990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12987..13009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12998..13014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13022..13028,13029..13046,13047..13052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13067..13088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13205..13222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
       61 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
      781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
      841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
      961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     1681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     1801 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     1861 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     1921 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     1981 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     2041 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     2101 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     2161 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     2221 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     2281 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     2341 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     2401 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     2461 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     2521 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     2581 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
     2641 cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt
     2701 ggtaccgagc tcggatccag gatggactac aaggacgacg atgacaaggg ggaacatggc
     2761 ctggagctgg cttccatgat ccccgccctg cgggagctgg gcagtgccac accagaggaa
     2821 tataatacag ttgtacagaa gccaagacaa attctgtgtc aattcattga ccggatactt
     2881 acagatgtaa atgttgttgc tgtagaactt gtaaagaaaa ctgactctca gccaacctcc
     2941 gtgatgttgc ttgatttcat ccagcatatc atgaaatcct ccccacttat gtttgtaaat
     3001 gtgagtggaa gccatgaggc caaaggcagt tgtattgaat tcagtaattg gatcataacg
     3061 agacttctgc ggattgcagc aactccctcc tgtcatttgt tacacaagaa aatctgtgaa
     3121 gtcatctgtt cattattatt tctttttaaa agcaagagtc ctgctatttt tggggtcctc
     3181 acaaaagaat tattacaact ttttgaagac ttggtttacc tccatagaag aaatgtgatg
     3241 ggtcatgctg tggaatggcc agtggtcatg agccgatttt taagtcaatt agatgaacac
     3301 atgggatatt tacaatcagc tcctttgcag ttgatgagta tgcaaaattt agaatttatt
     3361 gaagtcactt tattaatggt tcttactcgt attattgcaa ttgtgttttt tagaaggcaa
     3421 gaactcttac tttggcagat aggttgtgtt ctgctagagt atggtagtcc aaaaattaaa
     3481 tccctagcaa ttagcttttt aacagaactt tttcagcttg gaggactacc agcacaacca
     3541 gctagcactt ttttcagctc atttttggaa ttattaaaac accttgtaga aatggatact
     3601 gaccaattga aactctatga agagccatta tcaaagctga taaagacact atttcccttt
     3661 gaagcagaag cttatagaaa tattgaacct gtctatttaa atatgctgct ggaaaaactc
     3721 tgtgtcatgt ttgaagacgg tgtgctcatg cggcttaagt ctgatttgct aaaagcagct
     3781 ttgtgccatt tactgcagta tttccttaaa tttgtgccag ctgggtatga atctgcttta
     3841 caagtcagga aggtctatgt gagaaatatt tgtaaagctc ttttggatgt gcttggaatt
     3901 gaggtagatg cagagtactt gttgggccca ctttatgcag ctttgaaaat ggaaagtatg
     3961 gaaatcattg aggagattca atgccaaact caacaggaaa acctcagcag taatagtgat
     4021 ggaatatcac ccaaaaggcg tcgtctcagc tcgtctctaa acccttctaa aagagcacca
     4081 aaacagactg aggaaattaa acatgtggac atgaaccaaa agagcatatt atggagtgca
     4141 ctgaaacaga aagctgaatc ccttcagatt tcccttgaat acagtggcct aaagaatcct
     4201 gttattgaga tgttagaagg aattgctgtt gtcttacaac tgactgctct gtgtactgtt
     4261 cattgttctc atcaaaacat gaactgccgt actttcaagg actgtcaaca taaatccaag
     4321 aagaaacctt ctgtagtgat aacttggatg tcattggatt tttacacaaa agtgcttaag
     4381 agctgtagaa gtttgttaga atctgttcag aaactggacc tggaggcaac cattgataag
     4441 gtggtgaaaa tttatgatgc tttgatttat atgcaagtaa acagttcatt tgaagatcat
     4501 atcctggaag atttatgtgg tatgctctca cttccatgga tttattccca ttctgatgat
     4561 ggctgtttaa agttgaccac atttgccgct aatcttctaa cattaagctg taggatttca
     4621 gatagctatt caccacaggc acaatcacga tgtgtgtttc ttctgactct gtttccaaga
     4681 agaatattcc ttgagtggag aacagcagtt tacaactggg ccctgcagag ctcccatgaa
     4741 gtaatccggg ctagttgtgt tagtggattt tttatcttat tgcagcagca gaattcttgt
     4801 aacagagttc ccaagattct tatagataaa gtcaaagatg attctgacat tgtcaagaaa
     4861 gaatttgctt ctatacttgg tcaacttgtc tgtactcttc acggcatgtt ttatctgaca
     4921 agttctttaa cagaaccttt ctctgaacac ggacatgtgg acctcttctg taggaacttg
     4981 aaagccactt ctcaacatga atgttcatct tctcaactaa aagcttctgt ctgcaagcca
     5041 ttccttttcc tactgaaaaa aaaaatacct agtccagtaa aacttgcttt catagataat
     5101 ctacatcatc tttgtaagca tcttgatttt agagaagatg aaacagatgt aaaagcagtt
     5161 cttggaactt tattaaattt aatggaagat ccagacaaag atgttagagt ggcttttagt
     5221 ggaaatatca agcacatatt ggaatccttg gactctgaag atggatttat aaaggagctt
     5281 tttgtcttaa gaatgaagga agcatataca catgcccaaa tatcaagaaa taatgagctg
     5341 aaggatacct tgattcttac aacaggggat attggaaggg ccgcaaaagg agatttggta
     5401 ccatttgcac tcttacactt attgcattgt ttgttatcca agtcagcatc tgtctctgga
     5461 gcagcataca cagaaattag agctctggtt gcagctaaaa gtgttaaact gcaaagtttt
     5521 ttcagccagt ataagaaacc catctgtcag tttttggtag aatcccttca ctctagtcag
     5581 atgacagcac ttccgaatac tccatgccag aatgctgacg tgcgaaaaca agatgtggct
     5641 caccagagag aaatggcttt aaatacgttg tctgaaattg ccaacgtttt cgactttcct
     5701 gatcttaatc gttttcttac taggacatta caagttctac tacctgatct tgctgccaaa
     5761 gcaagccctg cagcttctgc tctcattcga actttaggaa aacaattaaa tgtcaatcgt
     5821 agagagattt taataaacaa cttcaaatat attttttctc atttggtctg ttcttgttcc
     5881 aaagatgaat tagaacgtgc ccttcattat ctgaagaatg aaacagaaat tgaactgggg
     5941 agcctgttga gacaagattt ccaaggattg cataatgaat tattgctgcg tattggagaa
     6001 cactatcaac aggtttttaa tggtttgtca atacttgcct catttgcatc cagtgatgat
     6061 ccatatcagg gcccgagaga tatcatatca cctgaactga tggctgatta tttacaaccc
     6121 aaattgttgg gcattttggc tttttttaac atgcagttac tgagctctag tgttggcatt
     6181 gaagataaga aaatggcctt gaacagtttg atgtctttga tgaagttaat gggacccaaa
     6241 catgtcagtt ctgtgagggt gaagatgatg accacactga gaactggcct tcgattcaag
     6301 gatgattttc ctgaattgtg ttgcagagct tgggactgct ttgttcgctg cctggatcat
     6361 gcttgtctgg gctcccttct cagtcatgta atagtagctt tgttacctct tatacacatc
     6421 cagcctaaag aaactgcagc tatcttccac tacctcataa ttgaaaacag ggatgctgtg
     6481 caagattttc ttcatgaaat atatttttta cctgatcatc cagaattaaa aaagataaaa
     6541 gccgttctcc aggaatacag aaaggagacc tctgagagca ctgatcttca gacaactctt
     6601 cagctctcta tgaaggccat tcaacatgaa aatgtcgatg ttcgtattca tgctcttaca
     6661 agcttgaagg aaaccttgta taaaaatcag gaaaaactga taaagtatgc aacagacagt
     6721 gaaacagtag aacctattat ctcacagttg gtgacagtgc ttttgaaagg ttgccaagat
     6781 gcaaactctc aagctcggtt gctctgtggg gaatgtttag gggaattggg ggcgatagat
     6841 ccaggtcgat tagatttctc aacaactgaa actcaaggaa aagattttac atttgtgact
     6901 ggagtagaag attcaagctt tgcctatgga ttattgatgg agctaacaag agcttacctt
     6961 gcgtatgctg ataatagccg agctcaagat tcagctgcct atgccattca ggagttgctt
     7021 tctatttatg actgtagaga gatggagacc aacggcccag gtcaccaatt gtggaggaga
     7081 tttcctgagc atgttcggga aatactagaa cctcatctaa ataccagata caagagttct
     7141 cagaagtcaa ccgattggtc tggagtaaag aagccaattt acttaagtaa attgggtagt
     7201 aactttgcag aatggtcagc atcttgggca ggttatctta ttacaaaggt tcgacatgat
     7261 cttgccagta aaattttcac ctgctgtagc attatgatga agcatgattt caaagtgacc
     7321 atctatcttc ttccacatat tctggtgtat gtcttactgg gttgtaatca agaagatcag
     7381 caggaggttt atgcagaaat tatggcagtt ctaaagcatg acgatcagca taccataaat
     7441 acccaagaca ttgcatctga tctgtgtcaa ctcagtacac agactgtgtt ctccatgctt
     7501 gaccatctca cacagtgggc aaggcacaaa tttcaggcac tgaaagctga gaaatgtcca
     7561 cacagcaaat caaacagaaa taaggtagac tcaatggtat ctactgtgga ttatgaagac
     7621 tatcagagtg taacccattt tctagacctc ataccccagg atactctggc agtagcttcc
     7681 tttcgctcca aagcatacac acgagctgta atgcactttg aatcatttat tacagaaaag
     7741 aagcaaaata ttcaggaaca tcttggattt ttacagaaat tgtatgctgc tatgcatgaa
     7801 cctgatggag tggccggagt cagtgcaatt agaaaggcag aaccatctct aaaagaacag
     7861 atccttgaac atgaaagcct tggcttgctg agggatgcca ctgcttgtta tgacagggct
     7921 attcagctag aaccagacca gatcattcat tatcatggtg tagtaaagtc catgttaggt
     7981 cttggtcagc tgtctactgt tatcactcag gtgaatggag tgcatgctaa caggtccgag
     8041 tggacagatg aattaaacac gtacagagtg gaagcagctt ggaaattgtc acagtgggat
     8101 ttggtggaaa actatttggc agcagatgga aaatctacaa catggagtgt cagactggga
     8161 cagctattat tatcagccaa aaaaagagat atcacagctt tttatgactc actgaaacta
     8221 gtgagagcag aacaaattgt acctctttca gctgcaagct ttgaaagagg ctcctaccaa
     8281 cgaggatatg aatatattgt gagattgcac atgttatgtg agttggagca tagcatcaaa
     8341 ccacttttcc agcattctcc aggtgacagt tctcaagaag attctctaaa ctgggtagct
     8401 cgactagaaa tgacccagaa ttcctacaga gccaaggagc ctatcctggc tctccggagg
     8461 gctttactaa gcctcaacaa aagaccagat tacaatgaaa tggttggaga atgctggctg
     8521 cagagtgcca gggtagctag aaaggctggt caccaccaga cagcctacaa tgctctcctt
     8581 aatgcagggg aatcacgact cgctgaactg tacgtggaaa gggcaaagtg gctctggtcc
     8641 aagggtgatg ttcaccaggc actaattgtt cttcaaaaag gtgttgaatt atgttttcct
     8701 gaaaatgaaa ccccacctga gggtaagaac atgttaatcc atggtcgagc tatgctacta
     8761 gtgggccgat ttatggaaga aacagctaac tttgaaagca atgcaattat gaaaaaatat
     8821 aaggatgtga ccgcgtgcct gccagaatgg gaggatgggc atttttacct tgccaagtac
     8881 tatgacaaat tgatgcccat ggtcacagac aacaaaatgg aaaagcaagg tgatctcatc
     8941 cggtatatag ttcttcattt tggcagatct ctacaatatg gaaatcagtt catatatcag
     9001 tcaatgccac gaatgttaac tctatggctt gattatggta caaaggcata tgaatgggaa
     9061 aaagctggcc gctccgatcg tgtacaaatg aggaatgatt tgggtaaaat aaacaaggtt
     9121 atcacagagc atacaaacta tttagctcca tatcaatttt tgactgcttt ttcacaattg
     9181 atctctcgaa tttgtcattc tcacgatgaa gtttttgttg tcttgatgga aataatagcc
     9241 aaagtatttc tagcctatcc tcaacaagca atgtggatga tgacagctgt gtcaaagtca
     9301 tcttatccca tgcgtgtgaa cagatgcaag gaaatcctca ataaagctat tcatatgaaa
     9361 aaatccttag agaagtttgt tggagatgca actcgcctaa cagataagct tctagaattg
     9421 tgcaataaac cggttgatgg aagtagttcc acattaagca tgagcactca ttttaaaatg
     9481 cttaaaaagc tggtagaaga agcaacattt agtgaaatcc tcattcctct acaatcagtc
     9541 atgataccta cacttccatc aattctgggt acccatgcta accatgctag ccatgaacca
     9601 tttcctggac attgggccta tattgcaggg tttgatgata tggtggaaat tcttgcttct
     9661 cttcagaaac caaagaagat ttctttaaaa ggctcagatg gaaagttcta catcatgatg
     9721 tgtaggccaa aagatgacct gagaaaggat tgtagactaa tggaattcaa ttccttgatt
     9781 aataagtgct taagaaaaga tgcagagtct cgtagaagag aacttcatat tcgaacatat
     9841 gcagttattc cactaaatga tgaatgtggg attattgaat gggtgaacaa cactgctggt
     9901 ttgagaccta ttctgaccaa actatataaa gaaaagggag tgtatatgac aggaaaagaa
     9961 cttcgccagt gtatgctacc aaagtcagca gctttatctg aaaaactcaa agtattccga
    10021 gaatttctcc tgcccaggca tcctcctatt tttcatgagt ggtttctgag aacattccct
    10081 gatcctacat catggtacag tagtagatca gcttactgcc gttccactgc agtaatgtca
    10141 atggttggtt atattctggg gcttggagac cgtcatggtg aaaatattct ctttgattct
    10201 ttgactggtg aatgcgtaca tgtagatttc aattgtcttt tcaataaggg agaaaccttt
    10261 gaagttccag aaattgtgcc atttcgcctg actcataata tggttaatgg aatgggtcct
    10321 atgggaacag agggtctttt tcgaagagca tgtgaagtta caatgaggct gatgcgtgat
    10381 cagcgagagc ctttaatgag tgtcttaaag acttttctac atgatcctct tgtggaatgg
    10441 agtaaaccag tgaaagggca ttccaaagcg ccactgaatg aaactggaga agttgtcaat
    10501 gaaaaggcca agacccatgt tcttgacatt gagcagcgac tacaaggtgt aatcaagact
    10561 cgaaatagag tgacaggact gccgttatct attgaaggac atgtgcatta ccttatacag
    10621 gaagctactg atgaaaactt actatgccag atgtatcttg gttggactcc atatatgtga
    10681 ctcgagtcta gctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
    10741 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
    10801 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
    10861 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
    10921 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
    10981 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    11041 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    11101 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    11161 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
    11221 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    11281 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    11341 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    11401 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
    11461 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    11521 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    11581 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    11641 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    11701 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    11761 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
    11821 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
    11881 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
    11941 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
    12001 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
    12061 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
    12121 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
    12181 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
    12241 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
    12301 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
    12361 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
    12421 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
    12481 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
    12541 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
    12601 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
    12661 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
    12721 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    12781 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    12841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12901 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    12961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    13021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    13081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    13141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    13201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    13261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    13321 gagcaaaagg ccagcaaaag gccagga
//
