LOCUS       sequence-198794-        3301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AraC protein in E. coli. The expression is controlled by a
            constitutive promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Guo S, Murray RM
  TITLE     Construction of Incoherent Feedforward Loop Circuits in a Cell-Free 
            System and in Cells.
  JOURNAL   ACS Synth Biol. 2019 Mar 15;8(3):606-610. doi: 
            10.1021/acssynbio.8b00493. Epub 2019 Mar 6.
  PUBMED    30790525
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00493"; journalName: "ACS Synth Biol"; date: 
            "2019-03-15- 15"; volume: "8"; issue: "3"; pages: "606-610"
COMMENT     Sequence Label: pSG28-J23151-AraC
FEATURES             Location/Qualifiers
     source          1..3301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             122..781
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(188..207)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      802..896
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1303..1320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1437..1982)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1517..1536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2244..3122)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 atgcatgttt gtcttcttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat
       61 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
      121 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      181 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      241 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      301 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
      361 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      421 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
      481 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
      541 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
      601 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
      661 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
      721 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
      781 atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
      841 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
      901 gcctccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
      961 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     1021 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     1081 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
     1141 cgtaacggca aaagcaccgc cggacatcag cggtagcgga gtgtatactg gcttactatg
     1201 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
     1261 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     1321 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     1381 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     1441 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     1501 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     1561 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     1621 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     1681 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1741 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     1801 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     1861 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     1921 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     1981 aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt
     2041 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag
     2101 cttatcatcg ataagcttcc gatggcgcgc cgagaggctt tacactttat gcttccggct
     2161 gaagacaagg cacgactctc aagggcgcaa taaaaaagcc cccggaaggt gatcttccgg
     2221 gggctttctc atgcgttagt tcattatgac aacttgacgg ctacatcatt cactttttct
     2281 tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag
     2341 aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg
     2401 gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta
     2461 atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt
     2521 gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc
     2581 tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc
     2641 agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc
     2701 ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg
     2761 cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg
     2821 cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag
     2881 tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt
     2941 ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt
     3001 cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc
     3061 gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc
     3121 atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg cattagaaac
     3181 tttcctcagc atgattaaga tgtttcagta cgaaaattgc tttcattgtt gatctccttt
     3241 ttaagtgaac ttgggcccgc ttgcattgta cctaggactg agctagccat caagaacagc
     3301 a
//
