LOCUS       sequence-198796-        3542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses deGFP fluorescent protein in E. coli. The expression is 
            controlled by a pGlnA promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Guo S, Yeung E, Murray RM
  TITLE     Implementation and System Identification of a Phosphorylation-Based 
            Insulator in a Cell-Free Transcription-Translation System
  JOURNAL   bioRxiv 122606
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            122606"
COMMENT     Sequence Label: pSG05-pGlnA-deGFP
FEATURES             Location/Qualifiers
     source          1..3542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(375..396)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(636..655)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     983..1004
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     rep_origin      1309..1854
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1755..1774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1971..1988
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(join(2221..3012,3013..3081))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2844..2863
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3082..3186)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3254..3272
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcatcttat taatcagata aaatatttct agagcatcct ccgcaaacaa gtattgcaga
       61 gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc attgcaccaa
      121 catggtgctt aatgtttcca ttgaagcact atattggtgc aacattcaca tcgtggtgca
      181 gcccttttgc acgatggtgc gcatgataac gccttttagg ggcaatttaa aagttggcac
      241 agatttcgct ttatcttttt tacggcgaca cggccaaaat aattgcagat ttcgttacca
      301 cgacgaccta actttcaatt ctaataagga ggaagacttc aaccatggag cttttcactg
      361 gcgttgttcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      421 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      481 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      541 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      601 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      661 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      721 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      781 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      841 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      901 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      961 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatct
     1021 aactcgagca aagcccgccg aaaggcgggc ttttctgtgt cgaccgatgc ccttgagagc
     1081 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     1141 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcatgataag ctgtcaaaca
     1201 tgagaattgc aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa
     1261 ttgcactgaa atctagaaat attttatctg attaataaga tgatcttctt gagatcgttt
     1321 tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc
     1381 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc
     1441 accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc
     1501 taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact
     1561 caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac
     1621 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     1681 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg
     1741 agggagccgc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac
     1801 tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc
     1861 tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc
     1921 cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag
     1981 tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct
     2041 tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc
     2101 cagtatacac tccgctaggg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2161 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2221 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2281 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2341 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2401 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2461 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2521 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2581 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2641 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2701 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2761 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2821 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2881 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2941 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3001 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3061 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3121 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3181 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3241 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tctggcgaat
     3301 cctctgacca gccagaaaac gacctttctg tggtgaaacc ggatgctgca attcagagcg
     3361 ccagcaagtg ggggacagca gaagacctga ccgccgcaga gtggatgttt gacatggtga
     3421 agactatcgc accatcagcc agaaaaccga attttgctgg gtgggctaac gatatccgcc
     3481 tgatgcgtga acgtgacgga cgtaaccacc gcgacatgtg tgtgctgttc cgctgggcat
     3541 gc
//
