LOCUS       sequence-198804-       20329 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracyclin inducible expression construct for dCas9 fused to 
            GFP-binding Protein (N-terminally) and mRFP (C-terminus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 20329)
  AUTHORS   Anton T, Bultmann S
  TITLE     Site-specific recruitment of epigenetic factors with a modular 
            CRISPR/Cas system.
  JOURNAL   Nucleus. 2017 May 4;8(3):279-286. doi: 
            10.1080/19491034.2017.1292194. Epub 2017 Feb 23.
  PUBMED    28448738
REFERENCE   2  (bases 1 to 20329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2017.1292194"; journalName: "Nucleus"; date: 
            "2017-05-4- 4"; volume: "8"; issue: "3"; pages: "279-286"
COMMENT     Sequence Label: pRTS-GBP-dCas9-mRFP
FEATURES             Location/Qualifiers
     source          1..20329
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     336..358
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(504..1121,1122..1127,1128..1163,1164..1166,1167..1202,
                     1203..1205,1206..1241,1242..1250)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1977..1996)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             2540..2560
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2663..2857
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     polyA_signal    2928..3062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3336..3355)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     polyA_signal    3395..3529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3759..4358)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3842..3862)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4339..4358
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4433..4759)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4447..4579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4498..4517)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4744..4764
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4954..5542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5034..5053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5713..6504,6505..6573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6336..6355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6574..6678)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8805..10594
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     polyA_signal    11477..11611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(11635..12348,12349..12351,12352..12354))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(11638..12348,12349..12351,12352..12354))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11680..11701)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     12029..12048
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12288..12309
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    join(12516..12534,12535..12557,12558..12576,12577..12599,
                     12600..12618,12619..12641,12642..12660,12661..12683,
                     12684..12702,12703..12725,12726..12744,12745..12767,
                     12768..12786)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        12819..12857
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             13025..13366
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     CDS             13025..13366
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     CDS             join(13391..13417,13418..13420,13421..15904,15905..15910,
                     15911..15976,15977..15979,15980..17494)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(13391..13417,13418..13420,13421..15907,15908..15910,
                     15911..17494)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13391..17494
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             17507..17527
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      17583..17592
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17603..17623
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             17624..18298
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             17624..18298
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(17754..17772)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     18022..18041
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     18216..18239
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             18323..18346
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          18410..18982
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(19118..19137)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    19183..19238
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(19237..19256)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
       61 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
      121 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
      181 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
      241 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
      301 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
      361 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
      421 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcgtcg acgggatcct
      481 ggccagctac tagcgaattc accatgtcta gactggacaa gagcaaagtc ataaacggcg
      541 ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
      601 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
      661 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
      721 agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac
      781 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
      841 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
      901 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
      961 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     1021 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     1081 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg
     1141 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     1201 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctattaaaac
     1261 agctctgggg ttgtacccac cccagaggcc cacgtggcgg ctagtactcc ggtattgcgg
     1321 tacccttgta cgcctgtttt atactccctt cccgtaactt agacgcacaa aaccaagttc
     1381 aatagaaggg ggtacaaacc agtaccacca cgaacaagca cttctgtttc cccggtgatg
     1441 tcgtatagac tgcttgcgtg gttgaaagcg acggatccgt tatccgctta tgtacttcga
     1501 gaagcccagt accacctcgg aatcttcaat gcgttgcgct cagcactcaa ccccagagtg
     1561 tagcttaggc tgatgagtct ggacatccct caccggtgac ggtggtccag gctgcgttgg
     1621 cggcctacct atggctaacg ccatgggacg ctagttgtga acaaggtgtg aagagcctat
     1681 tgagctacat aagaatcctc cggcccctga atgcggctaa tcccaacctc ggagcaggtg
     1741 gtcacaaacc agtgattggc ctgtcgtaac gcgcaagtcc gtggcggaac cgactacttt
     1801 gggtgtccgt gtttcctttt attttattgt ggctgcttat ggtgacaatc acagattgtt
     1861 atcataaagc gaattggatt cccgggtaac taagtaagga tccatgtcta gattagataa
     1921 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     1981 aacccgtaaa ctcgcccaga agcttggtgt agagcagcct acattgtatt ggcatgtaaa
     2041 aaataagcgg gcccttatga acatgctttc agaggcaata ctggcgaagc atcacacccg
     2101 ttcagcaccg ttaccgactg agagttggca gcagtttctc caggaaaatg ctctgagttt
     2161 ccgtaaagca ttactggtcc atcgtgatgg agcccgattg catataggga cctctcctac
     2221 gcccccccag tttgaacaag cagaggcgca actacgctgt ctatgcgatg cagggttttc
     2281 ggtcgaggag gctcttttca ttctgcaatc tatcagccat tttacgttgg gtgcagtatt
     2341 agaggagcaa gcaacaaacc agatagaaaa taatcatgtg atagacgctg caccaccatt
     2401 attacaagag gcatttaata ttcaggcgag aacctctgct gaaatggcct tccatttcgg
     2461 gctgaaatca ttaatatttg gattttctgc acagttagat gaaaaaaagc atacacccat
     2521 tgaggatggt aataaatcgc caaaaaagaa gagaaaggtc gacggcggtg gtgctttgtc
     2581 tcctcagcac tctgctgtca ctcaaggaag tatcatcaag aacaaggagg gcatggatgc
     2641 taagtcacta actgcctggt cccggacact ggtgaccttc aaggatgtat ttgtggactt
     2701 caccagggag gagtggaagc tgctggacac tgctcagcag atcgtgtaca gaaatgtgat
     2761 gctggagaac tataagaacc tggtttcctt gggttatcag cttactaagc cagatgtgat
     2821 cctccggttg gagaagggag aagagccctg gctggtggag agagaaattc accaagagac
     2881 ccatcctgat tcagagactg catttgaaat caaatcatca gtttaaagat ccagacatga
     2941 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3001 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3061 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     3121 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct gcaagcctcg
     3181 tcgtcctggc cggaccacgc tatctgtgca aggtccccgg ccccggacgc gcgagcttgg
     3241 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     3301 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     3361 gtacctataa ccagaccgtt cagttgcggc cgcagatcca gacatgataa gatacattga
     3421 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3481 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3541 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     3601 aaacctctac aaatgtggta tggctgatta tgatcctcta gagtcggtgg gcctcggggg
     3661 cgggtgcggg gtcggcgggg ccgccccggg tggcttcggt cggagccatg gggtcgtgcg
     3721 ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc
     3781 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     3841 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     3901 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     3961 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     4021 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     4081 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     4141 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     4201 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccgggtcgac ggtgtggcgc
     4261 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggcccgggg gacgtcgtcg
     4321 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg aaggtcgtct ccttgtgagg
     4381 ggtcaggggc gtgggtcagg ggatggtggc ggcaccgggc ggatctgagc tttttgcaaa
     4441 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgcggcct
     4501 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     4561 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     4621 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     4681 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     4741 cacaccctaa ctgacacaca ttccacagcc aagctcggta tacccgacgc gaggctggat
     4801 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     4861 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggcc agcaaaaggc
     4921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     5401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5641 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6001 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     6061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6301 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     6361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     6721 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     6781 attctcatgt ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt
     6841 tcttccgttg ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc
     6901 cgagaatccc catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt
     6961 gaggctcagg acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt
     7021 ggtgatgagg acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac
     7081 agcgaccatg aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc
     7141 ctgtattcac tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat
     7201 gtctgacgag gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc
     7261 tggaccagaa ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg
     7321 aggacgggga agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg
     7381 atcagggcca agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg
     7441 ctgcaaaggg acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg
     7501 aggggcagga gcaggaggag gggcaggagc aggaggaggn ncaggagcag gaggtggagg
     7561 ccggggtcga ggaggcagtg gaggccgggg tcgaggaggt agtggaggcc ggggtcgagg
     7621 aggtagtgga ggccgccggg gtagaggacg tgaaagagcc agggggggaa gtcgtgaaag
     7681 agccaggggg agaggtcgtg gacgtggaga aaagaggccc aggagtccca gtagtcagtc
     7741 atcatcatcc gggtctccac cgcgcaggcc ccctccaggt agaaggccat ttttccaccc
     7801 tgtaggggaa gccgattatt ttgaatacca ccaagaaggt ggcccagatg gtgagcctga
     7861 cgtgcccccg ggagcgatag agcagggccc cgcagatgac ccaggagaag gcccaagcac
     7921 tggaccccgg ggtcagggtg atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca
     7981 tcgtggtcaa ggaggttcca acccgaaatt tgagaacatt gcagaaggtt taagagctct
     8041 cctggctagg agtcacgtag aaaggactac cgacgaagga acttgggtcg ccggtgtgtt
     8101 cgtatatgga ggtagtaaga cctcccttta caacctaagg cgaggaactg cccttgctat
     8161 tccacaatgt cgtcttacac cattgagtcg tctccccttt ggaatggccc ctggacccgg
     8221 cccacaacct ggcccgctaa gggagtccat tgtctgttat ttcatggtct ttttacaaac
     8281 tcatatattt gctgaggttt tgaaggatgc gattaaggac cttgttatga caaagcccgc
     8341 tcctacctgc aatatcaggg tgactgtgtg cagctttgac gatggagtag atttgcctcc
     8401 ctggtttcca cctatggtgg aaggggctgc cgcggagggt gatgacggag atgacggaga
     8461 tgaaggaggt gatggagatg agggtgagga agggcaggag tgatgtaact tgttaggaga
     8521 cgccctcaat cgtattaaaa gccgtgtatt cccccgcact aaagaataaa tccccagtag
     8581 acatcatgcg tgctgttggt gtatttctgg ccatctgtct tgtcaccatt ttcgtcctcc
     8641 caacatgggg caattgggca tacccatgtt gtcacgtcac tcagctccgc gctcaacacc
     8701 ttctcgcgtt ggaaaacatt agcgacattt acctggtgag caatcagaca tgcgacggct
     8761 ttagcctggc ctccttaaat tcacctaaga atgggagcaa ccagcaggaa aaggacaagc
     8821 agcgaaaatt cacgccccct tgggaggtgg cggcatatgc aaaggatagc actcccactc
     8881 tactactggg tatcatatgc tgactgtata tgcatgagga tagcatatgc tacccggata
     8941 cagattagga tagcatatac tacccagata tagattagga tagcatatgc tacccagata
     9001 tagattagga tagcctatgc tacccagata taaattagga tagcatatac tacccagata
     9061 tagattagga tagcatatgc tacccagata tagattagga tagcctatgc tacccagata
     9121 tagattagga tagcatatgc tacccagata tagattagga tagcatatgc tatccagata
     9181 tttgggtagt atatgctacc cagatataaa ttaggatagc atatactacc ctaatctcta
     9241 ttaggatagc atatgctacc cggatacaga ttaggatagc atatactacc cagatataga
     9301 ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc cagatataaa
     9361 ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc cagatataga
     9421 ttaggatagc ctatgctacc cagatataga ttaggatagc atatgctatc cagatatttg
     9481 ggtagtatat gctacccatg gcaacattag cccaccgtgc tctcagcgac ctcgtgaata
     9541 tgaggaccaa caaccctgtg cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag
     9601 atcgcagcaa tcgcgcccct atcttggccc gcccacctac ttatgcaggt attccccggg
     9661 gtgccattag tggttttgtg ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc
     9721 agccaagtta ttacaccctt attttacagt ccaaaaccgc agggcggcgt gtgggggctg
     9781 acgcgtgccc ccactccaca atttcaaaaa aaagagtggc cacttgtctt tgtttatggg
     9841 ccccattggc gtggagcccc gtttaatttt cgggggtgtt agagacaacc agtggagtcc
     9901 gctgctgtcg gcgtccactc tctttcccct tgttacaaat agagtgtaac aacatggttc
     9961 acctgtcttg gtccctgcct gggacacatc ttaataaccc cagtatcata ttgcactagg
    10021 attatgtgtt gcccatagcc ataaattcgt gtgagatgga catccagtct ttacggcttg
    10081 tccccacccc atggatttct attgttaaag atattcagaa tgtttcattc ctacactagt
    10141 atttattgcc caaggggttt gtgagggtta tattggtgtc atagcacaat gccaccactg
    10201 aaccccccgt ccaaatttta ttctgggggc gtcacctgaa accttgtttt cgagcacctc
    10261 acatacacct tactgttcac aactcagcag ttattctatt agctaaacga aggagaatga
    10321 agaagcaggc gaagattcag gagagttcac tgcccgctcc ttgatcttca gccactgccc
    10381 ttgtgactaa aatggttcac taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg
    10441 acagctcatg gggtgggaga tatcgctgtt ccttaggacc cttttactaa ccctaattcg
    10501 atagcatatg cttcccgttg ggtaacatat gctattgaat tagggttagt ctggatagta
    10561 tatactacta cccgggaagc atatgctacc cgtttagggt taacaagggg gccttataaa
    10621 cactattgct aatgccctct tgagggtccg cttatcggta gctacacagg cccctctgat
    10681 tgacgttggt gtagcctccc gtagtcttcc tgggcccctg ggaggtacat gtcccccagc
    10741 attggtgtaa gagcttcagc caagagttac acataaaggc aatgttgtgt tgcagtccac
    10801 agactgcaaa gtctgctcca ggatgaaagc cactcagtgt tggcaaatgt gcacatccat
    10861 ttataaggat gtcaactaca gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac
    10921 atgtggaaca gggcccagtt ggcaagttgt accaaccaac tgaagggatt acatgcactg
    10981 ccccgcgaag aaggggcaga gatgtcgtag tcaggtttag ttcgtccggg gcggggatcg
    11041 agatccggcg cgccacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    11101 gcgtatcacg aggccctttc gtcttcactc gacgcggcca gcttggtcga gctggatact
    11161 tcccgtccgc cagggggaca tgccggcgat gctgaaggtc gcgcgcattc ccgatgaaga
    11221 ggccggttac cgcctgttga cctggtggga cgggcagggc gccgcccgag tcttcgcctc
    11281 ggcggcgggc gctctgctca tggagcgcgc gtccggggcc ggggaccttg cacagatagc
    11341 gtggtccggc caggacgacg aggcttgcag gatcataatc agccatacca catttgtaga
    11401 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    11461 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    11521 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    11581 actcatcaat gtatcttatc atgtctggat cctctagaac taggtcgaca gatcttactt
    11641 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
    11701 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
    11761 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
    11821 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
    11881 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
    11941 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
    12001 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
    12061 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
    12121 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
    12181 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
    12241 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
    12301 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatccgcgg
    12361 ggatccacta gtatttaaat ctgcaggaat tcggggccgc ggaggctgga tcggtcccgg
    12421 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
    12481 aaacgagctc tgcttatata ggtcgagttt accactccct atcagtgata gagaaaagtg
    12541 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
    12601 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
    12661 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
    12721 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
    12781 tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg
    12841 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
    12901 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc
    12961 tcggtacccg gggatcctct agtcagcaag cttttcggcc tcactggccg gcgatatggc
    13021 cgatgtgcag ctggtggagt ctgggggagc cttggtgcag ccgggggggt ctctgagact
    13081 ctcctgtgca gcctctggat tccccgtcaa tcgctatagt atgaggtggt accgccaggc
    13141 tccagggaag gagcgcgagt gggtcgcggg tatgagtagt gctggtgatc gttcaagtta
    13201 tgaagactcc gtgaagggcc gattcaccat ctccagagac gacgccagga atacggtgta
    13261 tctgcaaatg aacagcctga aacctgagga cacggccgtg tattactgta atgtcaatgt
    13321 gggctttgag tactggggcc aggggaccca ggtcaccgtc tcctcaggta gcggcagcgg
    13381 tagctctaga atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg
    13441 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa
    13501 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga
    13561 gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa
    13621 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt
    13681 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc
    13741 aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca
    13801 tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc
    13861 gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga
    13921 caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga
    13981 agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc
    14041 caaatcccgg cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct
    14101 gtttggtaat cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga
    14161 cctggccgaa gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa
    14221 tctgctggcc cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc
    14281 agacgccatt ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct
    14341 gagcgctagt atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc
    14401 ccttgtcaga cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa
    14461 tggctacgcc ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa
    14521 gcccatcttg gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga
    14581 tctgttgcgc aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg
    14641 cgaactgcac gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag
    14701 ggaaaagatt gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg
    14761 gggaaattcc agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa
    14821 cttcgaggaa gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa
    14881 ctttgataaa aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta
    14941 cttcacagtt tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc
    15001 agcattcctg tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg
    15061 gaaagttacc gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc
    15121 tgttgaaatc agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct
    15181 cctgaaaatc attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga
    15241 ggacattgtc ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa
    15301 aacttacgct catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac
    15361 aggatggggg cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa
    15421 gacaatcctg gattttctta agtccgatgg atttgccaac aggaacttca tgcagttgat
    15481 ccatgatgac tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg
    15541 ggacagtctt cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat
    15601 actgcagacc gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga
    15661 gaatatcgtt atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag
    15721 tagggaaagg atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa
    15781 ggaacaccca gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca
    15841 gaacggcagg gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga
    15901 cgtggatgct atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt
    15961 gacaagatcc gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa
    16021 gaaaatgaaa aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt
    16081 cgataatctg actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat
    16141 caaaaggcag cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc
    16201 acgcatgaac accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac
    16261 tctgaagtct aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga
    16321 gatcaacaat taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact
    16381 tatcaaaaaa tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga
    16441 tgttaggaaa atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt
    16501 cttttacagc aatattatga attttttcaa gaccgagatt acactggcca atggagagat
    16561 tcggaagcga ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg
    16621 tagggatttc gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa
    16681 gaccgaagta cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga
    16741 caagctgatc gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc
    16801 tacagtcgct tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact
    16861 caaaagcgtc aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa
    16921 ccccatcgac tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa
    16981 gcttcccaag tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc
    17041 gggcgagctg cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta
    17101 tctggccagc cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct
    17161 gttcgtggaa caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc
    17221 caaaagagtg atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca
    17281 cagggataag cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa
    17341 cttgggcgcg cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac
    17401 ctctacaaag gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga
    17461 aacaagaatc gacctctctc agctcggtgg agacagcagg gctgacccca agaagaagag
    17521 gaaggtgatt gtacaagagc ggcctgagga gcagagccca ggcgagcaac agcgccgtgg
    17581 acgccaccat gggcgatcgc atccaaagaa gaagagaaag gtcatggcct cctccgagga
    17641 cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
    17701 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
    17761 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcca
    17821 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
    17881 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
    17941 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
    18001 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
    18061 caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa
    18121 gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc
    18181 cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga
    18241 ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgccgg
    18301 cctgtacaag atcgcggtta ccgattataa agatgatgat gataaataag gccagtgagg
    18361 ccgcatgcgt actgctgtcg actgagtctc tagagctgag aacttcaggg tgagtttggg
    18421 gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa
    18481 agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg
    18541 ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt
    18601 ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt
    18661 cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa
    18721 tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg
    18781 ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag
    18841 aaacaactac accctggtca tcatcctgcc tttctcttta tggttacaat gatatacact
    18901 gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca
    18961 tgccttcttc tctttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca
    19021 ttttggcaaa gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
    19081 ggccaatgcc ctggctcaca aataccactg agatcgatct ttttccctct gccaaaaatt
    19141 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
    19201 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
    19261 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
    19321 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
    19381 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
    19441 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
    19501 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
    19561 gatccctcga cctgcagccc aagctggaag atcccgtacg cctgcaggtt aattaaccct
    19621 aggaaaaata gttaaatact gtgactttaa aatgtgagag ggttttcaag tactcatttt
    19681 tttaaatgtc caaaattttt gtcaatcaat ttgaggtctt gtttgtgtag aactgacatt
    19741 acttaaagtt taaccgagga atgggagtga ggctctctca taccctattc agaactgact
    19801 tttaacaata ataaattaag tttaaaatat ttttaaatga attgagcaat gttgagttgg
    19861 agtcaagatg gccgatcaga accagaacac ctgcagcagc tggcaggaag caggtcatgt
    19921 ggcaaggcta tttggggaag ggaaaataaa accactaggt aaacttgtag ctgtggtttg
    19981 aagaagtggt tttgaaacac tctgtccagc cccaccaaac cgaaagtcca ggctgagcaa
    20041 aacaccacct gggtaatttg catttctaaa ataagttgag gattcagccg aaactggaga
    20101 ggtcctcttt taacttattg agttcaacct tttaatttta gcttgagtag ttctagtttc
    20161 cccaaactta agtttatcga cttctaaaat gtatttagaa ttggtttaaa cctcgaatgg
    20221 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
    20281 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcggg
//
