LOCUS       sequence-198811-        5650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector compatible with ColE1 plasmids with medium copy 
            number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Peterson J, Phillips GJ
  TITLE     New pSC101-derivative cloning vectors with elevated copy numbers.
  JOURNAL   Plasmid. 2008 May;59(3):193-201. doi: 10.1016/j.plasmid.2008.01.004.
            Epub 2008 Mar 4.
  PUBMED    18295880
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2008.01.004"; journalName: "Plasmid"; date: 
            "2008-05"; volume: "59"; issue: "3"; pages: "193-201"
COMMENT     Sequence Label: pJPC13
FEATURES             Location/Qualifiers
     source          1..5650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..108
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     17..33
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(68..84)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(68..84)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(121..141)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(121..139)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(160..176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(160..176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(173..195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    184..200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(208..214,215..232,233..238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    253..274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(391..408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      1553..1775
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1823..2773
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFRIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(3811..4470)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4385..4404
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     gap             4750..4796
                     /estimated_length=47
     rep_origin      complement(5001..5456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5138..5159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5239..5650,1..164))
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAG
                     FPRQALNRGLPLGFRFSALRHLDPKKLD"
     primer_bind     5350..5369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5583..5605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5597..5614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5598..5614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5624..5643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5624..5642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
       61 cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc
      121 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      181 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      241 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      301 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      361 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      421 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      481 aggggataac gcaggaaaga acatgtgaga tctctacggg tcggatttga agtcgtcttg
      541 gtaggaggca gcctgaatgg cgaatgccga tgcccttgag agccttcaac ccagtcagct
      601 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
      661 tgcaactcgt aggacagggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
      721 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
      781 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
      841 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
      901 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
      961 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     1021 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     1081 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     1141 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     1201 ggcacctcgc taacggattc accactccgc agacccgcca taaaacgccc tgagaagccc
     1261 gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa aaggcgcctg
     1321 tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg aatgtcacga
     1381 aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca gcgatttgcc
     1441 cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt gtagaggagc
     1501 aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta gtgagttata
     1561 cacagggctg ggatctattc tttttatctt tttttattct ttctttattc tataaattat
     1621 aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta cagagggtct
     1681 agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac ccacaactca
     1741 aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata gtgattaaaa
     1801 tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa atgaactagc
     1861 gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct gtgtggcact
     1921 actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt tcacttataa
     1981 ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat tagctaaagc
     2041 aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta aaggctttcg
     2101 gatcttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat tagtttttag
     2161 tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata atctggaaca
     2221 tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat taaaagaact
     2281 aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat ttaagttcat
     2341 gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg ttttgaaacc
     2401 aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata agcgaggccg
     2461 cccgactgat acgttgattt tccaagttga actagataga caaatggatc tcgtaaccga
     2521 acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca ttacatcaga
     2581 ttcctaccta cataacggac taagaaaaac actacacgat gctttaacag caaaaattca
     2641 gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagtatg atctcaatgg
     2701 ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac tggctaaata
     2761 cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca agactaacaa
     2821 acaaaagtag aacaactgtt caccgttaca tatcaaaggg aaaactgtcc atatgcacag
     2881 atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt ggtgcattca
     2941 aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta acacctaata
     3001 gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac ctgagacaac
     3061 ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg cttatcgaat
     3121 caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa tcatggcaat
     3181 tctggaagaa atagcgcttt cagccggcaa accggctgaa gccggatctg cgattctgat
     3241 aacaaactag caacaccaga acagcccgtt tgcgggcagc aaaacccgta cttttggacg
     3301 ttccggcggt tttttgtggc gagtggtgtt cgggcggtgc gcgcaagatc cattatgtta
     3361 aacgggcgag tttacatctc aaaaccgccc gcttaacacc atcagaaatc ctcagcgcga
     3421 ttttaagcac caaccccccc ccgtaacacc caaatccata ctgaaagtgg ctttgttgaa
     3481 taaatcagat ttcgggtaag tctcccccgt agcgggttgt gttttcaggc aatacgcacg
     3541 ctttcaggca tacctgcttt cgtcattttg ttcagcgctc gtaccagggc catagcctcc
     3601 gcaacctgac catcgtagtc acgcagcgtc agtgaacccc cgaacagaga tctgacgctc
     3661 agtggaacga aaactcacgt taagggattt tggtcatgag acaataaccc tgataaatgc
     3721 ttcaataatc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat
     3781 ttctaggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta
     3841 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     3901 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     3961 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     4021 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     4081 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     4141 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     4201 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     4261 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     4321 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     4381 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     4441 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     4501 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     4561 tacaaaagga tctaggtgaa gatccttttt gataatctca tgagcggata catatttgaa
     4621 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4681 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa ttttdagaat gcattttccg
     4741 agttaatacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcc
     4801 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     4861 tggtgaaagt tggaacctct tacaaaagga tctaggtgaa gatccttttt gataatctca
     4921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4981 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     5041 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     5101 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     5161 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     5221 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     5281 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     5341 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     5401 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     5461 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5521 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5581 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
     5641 gggcgaattg
//
