LOCUS       sequence-198812-        6762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW
  TITLE     Optimized large-scale production of high titer lentivirus vector 
            pseudotypes.
  JOURNAL   J Virol Methods. 2004 Dec 15. 122(2):131-9.
  PUBMED    15542136
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol 
            Methods. 2004 Dec 15. 122(2):131-9."
COMMENT     Sequence Label: pHCMV-EcoEnv
FEATURES             Location/Qualifiers
     source          1..6762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        113..492
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     647..667
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     693..717
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          808..1380
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3483..3502)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3548..3603
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3602..3621)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3961..3977)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3961..3977)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3974..3996)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3985..4001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4009..4015,4016..4033,4034..4039))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4054..4075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4192..4209)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4363..4951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4443..4462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5122..5913,5914..5982))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5745..5764
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5983..6087)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6113..6568)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6250..6271)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6462..6481
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6695..6717
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6709..6726
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6710..6726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6736..6755
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6736..6754
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggtggcgg ccgctctaga gagcttggcc cattgcatac gttgtatcca
       61 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
      121 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      181 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      241 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      301 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      361 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      421 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      481 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      541 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      601 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      661 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      721 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      781 cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt
      841 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga
      901 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt
      961 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg
     1021 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat
     1081 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact
     1141 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat
     1201 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac cctggtcatc
     1261 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac
     1321 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc tttcctacag
     1381 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccgcggg
     1441 cggccgctct agaggatccg ctagactgac atggcgcgtt caacgctctc aaaacccctt
     1501 aaaaataagg ttaacccgcg aggcccccta atccccttaa ttcttctgat gctcagaggg
     1561 gtcagtactg cttcgcccgg ctccagtcct catcaagtct ataatatcac ctgggaggta
     1621 accaatggag atcgggagac ggtatgggca acttctggca accaccctct gtggacctgg
     1681 tggcctgacc ttaccccaga tttatgtatg ttagcccacc atggaccatc ttattggggg
     1741 ctagaatatc aatccccttt ttcttctccc ccggggcccc cttgttgctc agggggcagc
     1801 agcccaggct gttccagaga ctgcgaagaa cctttaacct ccctcacccc tcggtgcaac
     1861 actgcctgga acagactcaa gctagaccag acaactcata aatcaaatga gggattttat
     1921 gtttgccccg ggccccaccg cccccgagaa tccaagtcat gtgggggtcc agactccttc
     1981 tactgtgcct attggggctg tgagacaacc ggtagagctt actggaagcc ctcctcatca
     2041 tgggatttca tcacagtaaa caacaatctc acctctgacc aggctgtcca ggtatgcaaa
     2101 gataataagt ggtgcaaccc cttagttatt cggtttacag acgccgggag acgggttact
     2161 tcctggacca caggacatta ctggggctta cgtttgtatg tctccggaca agatccaggg
     2221 cttacatttg ggatccgact cagataccaa aatctaggac cccgcgtccc aatagggcca
     2281 aaccccgttc tggcagacca acagccactc tccaagccca aacctgttaa gtcgccttca
     2341 gtcaccaaac cacccagtgg gactcctctc tcccctaccc aacttccacc ggcgggaacg
     2401 gaaaataggc tgctaaactt agtagacgga gcctaccaag ccctcaacct caccagtcct
     2461 gacaaaaccc aagagtgctg gttgtgtcta gtagcgggac ccccctacta cgaaggggtt
     2521 gccgtcctgg gtacctactc caaccatacc tctgctccag ccaactgctc cgtggcctcc
     2581 caacacaagt tgaccctgtc cgaagtgacc ggacagggac tctgcatagg agcagttccc
     2641 aaaacacatc aggccctatg taataccacc cagacaagca gtcgagggtc ctattatcta
     2701 gttgccccta caggtaccat gtgggcttgt agtaccgggc ttactccatg catctccacc
     2761 accatactga accttaccac tgattattgt gttcttgtcg aactctggcc aagagtcacc
     2821 tatcattccc ccagctatgt ttacggcctg tttgagagat ccaaccgaca caaaagagaa
     2881 ccggtgtcgt taaccctggc cctattattg ggtggactaa ccatgggggg aattgccgct
     2941 ggaataggaa cagggactac tgctctaatg gccactcagc aattccagca gctccaagcc
     3001 gcagtacagg atgatctcag ggaggttgaa aaatcaatct ctaacctaga aaagtctctc
     3061 acttccctgt ctgaagttgt cctacagaat cgaaggggcc tagacttgtt atttctaaaa
     3121 gaaggagggc tgtgtgctgc tctaaaagaa gaatgttgct tctatgcgga ccacacagga
     3181 ctagtgagag acagcatggc caaattgaga gagaggctta atcagagaca gaaactgttt
     3241 gagtcaactc aaggatggtt tgagggactg tttaacagat ccccttggtt taccaccttg
     3301 atatctacca ttatgggacc cctcattgta ctcctaatga ttttgctctt cggaccctgc
     3361 attcttaatc gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt
     3421 ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagct cgagggccca
     3481 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     3541 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     3601 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     3661 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     3721 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     3781 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     3841 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     3901 atagctgtcc ctcttctctt atggagatcc ctcgacggat cggccgcaat tcgtaatcat
     3961 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4021 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4081 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4141 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4201 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4261 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4321 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4381 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4441 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4501 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4561 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4621 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4681 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4741 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4801 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4861 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4921 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4981 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5041 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5101 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5161 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5221 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5281 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5341 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5401 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5461 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5521 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5581 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5641 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5701 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5761 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5821 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5881 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5941 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6001 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6061 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     6121 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     6181 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6241 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6301 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6361 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6421 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6481 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6541 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     6601 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     6661 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     6721 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tg
//
