LOCUS       sequence-198816-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Frank SB, Berger PL, Ljungman M, Miranti CK
  TITLE     Human prostate luminal cell differentiation requires NOTCH3 
            induction by p38-MAPK and MYC.
  JOURNAL   J Cell Sci. 2017 Apr 26. pii: jcs.197152. doi: 10.1242/jcs.197152.
  PUBMED    28446540
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2017 Apr 26. pii: jcs.197152. doi: 10.1242/jcs.197152."
COMMENT     Sequence Label: pLenti-Tet-MKK6(DD)-Puro
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..122
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(123..191,192..983)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(341..360)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1154..1742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1643..1662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1896..1913
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2030..2051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2066..2071,2072..2089,2090..2096)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2104..2120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2109..2131
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2128..2144
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2128..2144
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2163..2183
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2165..2183
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2211..2437
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2438..2618
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2665..2790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3283..3516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3701..3745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4012..4128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4151..4454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4455..4658
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4609..4629
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4660..4678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4681..4699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4740..4764
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4799..4828
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(6020..6044)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6075..6663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6128..6148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6546..6557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6677..7176
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6712..6735)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7109..7128
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7197..7796
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7197..7216)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7693..7713
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7934..8167
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8239..8360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8276..8295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8330..8349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8400..8535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8555..8574)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8556..8574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8584..8601)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8584..8600)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8593..8615)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8742..9197
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8829..8848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9039..9060
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
       61 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      121 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      181 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      241 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      301 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      361 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      421 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      481 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      541 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
      601 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
      661 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
      721 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
      781 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
      841 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
      901 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
      961 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1021 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1081 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1141 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1201 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1261 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1321 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1381 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1441 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1501 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1561 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1621 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1681 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1741 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1801 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1861 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1921 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1981 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2041 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2101 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     2161 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     2221 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     2281 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     2341 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     2401 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     2461 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2521 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2581 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     2641 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     2701 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     2761 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     2821 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     2881 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     2941 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     3001 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     3061 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     3121 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     3181 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     3241 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     3301 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     3361 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     3421 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     3481 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     3541 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     3601 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     3661 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     3721 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     3781 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     3841 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     3901 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     3961 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     4141 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     4201 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4261 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     4321 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4381 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4441 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4501 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4561 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4621 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
     4681 tccctatcag tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa
     4741 caagtttgta caaaaaagca ggctctttaa aggaaccaat tcagtcgaca ccatgccgga
     4801 gcagaagctg atatccgagg aggacctggc catggctcag tcgaaaggca agaagcgaaa
     4861 ccctggcctt aaaattccaa aagaagcatt tgaacaacct cagaccagtt ccacaccacc
     4921 tcgagattta gactccaagg cttgcatttc tattggaaat cagaactttg aggtgaaggc
     4981 agatgacctg gagcctataa tggaactggg acgaggtgcg tacggggtgg tggagaagat
     5041 gcggcacgtg cccagcgggc agatcatggc agtgaagcgg atccgagcca cagtaaatag
     5101 ccaggaacag aaacggctac tgatggattt ggatatttcc atgaggacgg tggactgtcc
     5161 attcactgtc accttttatg gcgcactgtt tcgggagggt gatgtgtgga tctgcatgga
     5221 gctcatggat acatcactag ataaattcta caaacaagtt attgataaag gccagacaat
     5281 tccagaggac atcttaggga aaatagcagt ttctattgta aaagcattag aacatttaca
     5341 tagtaagctg tctgtcattc acagagacgt caagccttct aatgtactca tcaatgctct
     5401 cggtcaagtg aagatgtgcg attttggaat cagtggctac ttggtggacg atgttgctaa
     5461 agatattgat gcaggttgca aaccatacat ggcccctgaa agaataaacc cagagctcaa
     5521 ccagaaggga tacagtgtga agtctgacat ttggagtctg ggcatcacga tgattgagtt
     5581 ggccatcctt cgatttccct atgattcatg gggaactcca tttcagcagc tcaaacaggt
     5641 ggtagaggag ccatcgccac aactcccagc agacaagttc tctgcagagt ttgttgactt
     5701 tacctcacag tgcttaaaga agaattccaa agaacggcct acatacccag agctaatgca
     5761 acatccattt ttcaccctac atgaatccaa aggaacagat gtggcatctt ttgtaaaact
     5821 gattcttgga gactaactcg agatcgatta gactagtcta gaaattcacc ccaccagtgc
     5881 aggctgccta tcagaaagtg gtggctggtg tggctaatgc cctggcccac aagtatcact
     5941 aagctcgctt tcttgctgtc caatttctat taaaggttcc tttgttccct aagtccgcgg
     6001 ccgcactcga gatatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt
     6061 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6121 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6181 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6241 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6301 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6361 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6421 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6481 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6541 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6601 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6661 cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     6721 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     6781 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     6841 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     6901 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     6961 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     7021 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     7081 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     7141 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     7201 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     7261 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     7321 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     7381 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     7441 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     7501 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     7561 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     7621 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     7681 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     7741 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     7801 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
     7861 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7921 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     7981 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8041 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8101 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8161 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8221 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8281 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8341 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8401 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8461 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8521 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     8581 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     8641 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     8701 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     8761 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8821 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8881 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8941 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9001 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9061 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     9121 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     9181 attaacgctt acaatttagg tggca
//
