LOCUS       sequence-198832-        3306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF695 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF695
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(649..748)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(765..784)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(766..784)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(789..805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(789..805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1236..2027
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2120..2708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2609..2628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2862..2879
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3038..3065)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3157..3243)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3292..3306,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3306,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagttgg catgggacta ttggcattca gggatgtggc tctagaattc tctccagagg
      181 agtgggaatg cctggaccca gctcagcgga gtttgtatag ggatgtgatg ttagagaact
      241 acagaaacct gatctccctt ggtgaggata gcttcaatat gcaattccta tttcacagtc
      301 ttgctatgtc taagccagaa ctgatcatct gtctggaggc aaggaaagag ccctggaacg
      361 tgaacacaga gaagacagcc aaacactcag ctttgtcttc ttatcttact gaagacattt
      421 tgccagagca gggcctgcaa gtttcattcc aaaaagtgat gctgagaaga tatgaaagat
      481 gttgtcttga gaaattacgc ttaaggaatg actgggaaat tccatgtgaa gatgtgcttg
      541 cttccccttt gccttctgcc atgattctaa gtttcctgag gcctccccag aagcagaagc
      601 atgtaaagcc cacagaaccg gtccagtcgt cgaccatcga gctgctttgc ccaactttct
      661 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      721 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
      781 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      841 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
      901 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
      961 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     1021 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1081 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     1141 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1201 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     1261 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1321 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     1381 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     1441 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     1501 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     1561 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     1621 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     1681 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     1741 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     1801 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     1861 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     1921 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     1981 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     2041 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2101 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2701 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2761 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2821 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2881 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     2941 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     3001 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     3061 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     3121 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     3181 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     3241 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
     3301 gacgtt
//
