LOCUS       sequence-198853-        6088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Duan L, Che D, Zhang K, Ong Q, Guo S, Cui B
  TITLE     Optogenetic control of molecular motors and organelle distributions 
            in cells.
  JOURNAL   Chem Biol. 2015 May 21;22(5):671-82. doi: 
            10.1016/j.chembiol.2015.04.014. Epub 2015 May 9.
  PUBMED    25963241
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2015.04.014"; journalName: "Chem Biol"; date: 
            "2015-05-21- 21"; volume: "22"; issue: "5"; pages: "671-82"
COMMENT     Sequence Label: Kif5A-CIBN
FEATURES             Location/Qualifiers
     source          1..6088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      606..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2349..2858
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     polyA_signal    2876..2997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2913..2932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2967..2986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3004..3459)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3141..3162)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3353..3372
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3486..3590
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3592..3949
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3592..3783
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3615..3635)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3800..3935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3984..4778
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3984..4778
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4038..4057)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4648..4667
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4966..4985)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5010..5057
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5386..5974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5875..5894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 agcttgccac catgggcgcg cctgaattcg cggagaccaa taacgaatgc agcatcaagg
      661 tgctttgccg atttcggccc ctgaaccagg ccgagatcct gcggggggac aagttcatcc
      721 ccattttcca aggggacgac agcgtcatta ttgggggaaa gccatatgtc ttcgaccgtg
      781 tattcccccc aaacacgact caggagcagg tttaccacgc ctgtgccatg cagatcgtca
      841 aagacgtcct tgctggctac aacggcacaa tttttgctta tggacagaca tcctcaggga
      901 aaacgcacac catggaggga aagctgcacg accctcagct catgggcatc attccccgga
      961 tcgcccgaga catcttcaac cacatctact ccatggatga gaaccttgag ttccacatta
     1021 aggtgtctta ctttgagatt tacctggata agatccgtga ccttttggat gtgaccaaga
     1081 caaacctgtc tgtgcatgag gacaaaaacc gggtgccgtt tgtcaagggt tgtaccgaac
     1141 gctttgtgtc cagcccagag gagattctgg atgtgattga tgaggggaag tcgaaccgtc
     1201 acgttgccgt caccaacatg aatgagcaca gctctcggag tcacagcatc ttcctcatca
     1261 acatcaagca ggagaacata gagacggagc agaagctcag cgggaaactg taccttgtgg
     1321 atctggccgg aagcgagaag gtcagcaaga caggagcaga gggagccgtt ctggatgagg
     1381 caaagaatat caataagtcg ctgtcggccc tggggaatgt gatctctgct ctggccgagg
     1441 gcactaaaag ctacgtgccg taccgtgaca gcaaaatgac gaggattctc caggactctc
     1501 tgggagggaa ctgcaggacc accatgttca tctgctgctc accatccagc tacaatgacg
     1561 cagagaccaa gtccacactc atgtttgggc agcgggcaaa gaccatcaag aacactgcct
     1621 cagtgaatct ggagttgact gctgagcaat ggaagaagaa atatgagaag gagaaggaga
     1681 agaccaaggc ccagaaggag acaattgcga agctggaggc tgagcttagc cggtggcgca
     1741 atggagagaa tgtgcctgag actgagcgcc tggctggaga ggactcagct ctggcagccg
     1801 agatctgtga ggagacccct gtgaatgaca actcatccat cgtggtacgc atcgcaccgg
     1861 aggaaaggca gaaatacgag gaagagatcc gccgtctcta caagcagctc gacgacaagg
     1921 atgatgagat caaccagcag agccaactca ttgagaagct gaagcagcag atgctggacc
     1981 aggaagagct gctagtgtcc actcggggag acaatgagaa ggtccagcgg gagctgagcc
     2041 acctgcagtc tgagaacgat gccgcgaagg aggaggtgaa ggaagtgttg caggccctgg
     2101 aggagctggc agtcaactat gaccagaagt cccaggaggt ggaggagaag agccagcaga
     2161 accagctgct ggtggatgag ctgtcccaga aagtggccac catgctgtcc ctggagtctg
     2221 agctacagcg gctccaggaa gtcagtggac accagcgaaa acggattgct gaggtgctga
     2281 atggactgat gaaggacctg agtgagttca gtgtcatcgt gggcaagtcg actatgtaca
     2341 agggatccat gaatggagct ataggaggtg accttttgct caattttcct gacatgtcgg
     2401 tcctagagcg ccaaagggct cacctcaagt acctcaatcc cacctttgat tctcctctcg
     2461 ccggcttctt tgccgattct tcaatgatta ccggcggcga gatggacagc tatctttcga
     2521 ctgccggttt gaatcttccg atgatgtacg gtgagacgac ggtggaaggt gattcaagac
     2581 tctcaatttc gccggaaacg acgcttggga ctggaaattt caaggcagcg aagtttgata
     2641 cagagactaa ggattgtaat gaggcggcga agaagatgac gatgaacaga gatgacctag
     2701 tagaagaagg agaagaagag aagtcgaaaa taacagagca aaacaatggg agcacaaaaa
     2761 gcatcaagaa gatgaaacac aaagccaaga aagaagagaa caatttctct aatgattcat
     2821 ctaaagtgac gaaggaattg gagaaaacgg attatattta acaattgttg ttgttaactt
     2881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     3001 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3061 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     3481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     3601 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     3661 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     3721 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     3781 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3841 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3901 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     3961 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4021 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4081 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4141 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     4201 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4261 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4321 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4381 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4441 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4501 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4561 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4621 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     4681 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     4741 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     4801 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     4861 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4921 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     4981 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     5041 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     5101 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     5161 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     5221 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5281 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5521 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6061 tgtggataac cgtattaccg ccatgcat
//
