LOCUS       sequence-198856-        6193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Duan L, Che D, Zhang K, Ong Q, Guo S, Cui B
  TITLE     Optogenetic control of molecular motors and organelle distributions 
            in cells.
  JOURNAL   Chem Biol. 2015 May 21;22(5):671-82. doi: 
            10.1016/j.chembiol.2015.04.014. Epub 2015 May 9.
  PUBMED    25963241
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2015.04.014"; journalName: "Chem Biol"; date: 
            "2015-05-21- 21"; volume: "22"; issue: "5"; pages: "671-82"
COMMENT     Sequence Label: BICDN-CIBN
FEATURES             Location/Qualifiers
     source          1..6193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2454..2963
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     polyA_signal    2981..3102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3018..3037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3072..3091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3109..3564)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3246..3267)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3458..3477
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3591..3695
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3697..4054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3697..3888
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3720..3740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3905..4040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4089..4883
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4089..4883
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4143..4162)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4753..4772
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5071..5090)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5115..5162
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5491..6079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5980..5999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gccaccatgt
      661 ccgcgccgtc ggaggaggag gagtatgcgc gtctggtgat ggaggcgcag ccggagtggc
      721 tgcgcgccga ggtgaagcgg ctgtcccacg agctggccga gaccacgcgt gagaagatcc
      781 aggcggccga gtatggactg gcggtgctgg aggagaagca ccagctcaag ctgcagttcg
      841 aggagctcga ggtggactat gaggccatcc gcagcgagat ggagcagctc aaagaggcat
      901 ttggccaggc acatacaaac cacaagaagg tggctgctga tggtgagagc cgggaggaga
      961 gcctgatcca ggagtcggcc tccaaggagc agtactacgt gcggaaggtg ctggagctgc
     1021 agacagagct gaaacagctg cgcaacgtcc tcaccaacac ccagtctgag aatgagcgcc
     1081 tcacgtctgt ggcccaggag ctaaaagaga tcaaccagaa tgtggagatc cagcgtggtc
     1141 gcctgcggga tgacatcaag gagtacaagt tccgggaggc ccgtctactt caggactact
     1201 cagagctgga ggaggagaac atcagcctgc agaaacaagt gtctgtgctc aggcagaacc
     1261 aggtggagtt tgagggcctc aagcatgaaa tcaagcgcct ggaggaggag acagagtacc
     1321 tcaacagcca gctagaggat gccatccggc ttaaggagat ctctgaacgg cagttggagg
     1381 aggcgctgga gacactgaag acagagcggg agcagaagaa caacctgcgc aaggagttgt
     1441 cgcactacat gagcatcaac gattccttct ataccagcca cctgcaggtc tccttagatg
     1501 gcctcaagtt cagtgatgat actgtcaccg cagagcccaa caatgacgcc gaagccctgg
     1561 tcaatggctt tgagcacagt ggcttggtca aatcatcctt ggacaacaag acatccacac
     1621 ccaggaagga tggcctagcc ccaccctccc ccagcctcgt ctctgacctg ctcagtgagc
     1681 tccacatatc tgagatccag aagctgaaac agcagctggt gcagatggag cgggagaagg
     1741 tgggcctgct ggcaacactg caggacacac agaagcagct ggagcaggct cggggaaccc
     1801 tctctgagca gcatgagaag gtgaatcgtc tcacagagaa ccttagtgcc ctccggcgcc
     1861 tgcaggctgg caaggagcga cagacctcgt tggataatga gaaggaccgt gacagccacg
     1921 aagacggtga ctactatgag gtggacatca atgggcctga gatcctggcc tgcaagtacc
     1981 acgtggctgt ggctgaggct ggcgagctcc gggagcagct caaggcgttg cgcagcacac
     2041 acgaagctcg cgaagcccag cacgcagaag aaaagggccg gtatgaggct gagggccagg
     2101 cactcactga gaagatctct ctgctggaga aggctagcca ccaggaccga gagctgctgg
     2161 cccatctgga aaaggagctg aagaaggtga gcgatgtggc tggtgagacc cagggcagcc
     2221 tgaatgtggc tcaggatgag ctggtgacct tcagcgagga gctggccaac ctctaccacc
     2281 atgtgtgcat gtgcaacaac gagacgccca accgtgtcat gctcgactat tatcgtgagg
     2341 gccagggcaa ggctggccgc accagcccag agggccgcgg gcgccggtca cctgtcctct
     2401 tgcccaaggg gctgttggcc acagaggtgg gccgagaatt gtacaaggga tccatgaatg
     2461 gagctatagg aggtgacctt ttgctcaatt ttcctgacat gtcggtccta gagcgccaaa
     2521 gggctcacct caagtacctc aatcccacct ttgattctcc tctcgccggc ttctttgccg
     2581 attcttcaat gattaccggc ggcgagatgg acagctatct ttcgactgcc ggtttgaatc
     2641 ttccgatgat gtacggtgag acgacggtgg aaggtgattc aagactctca atttcgccgg
     2701 aaacgacgct tgggactgga aatttcaagg cagcgaagtt tgatacagag actaaggatt
     2761 gtaatgaggc ggcgaagaag atgacgatga acagagatga cctagtagaa gaaggagaag
     2821 aagagaagtc gaaaataaca gagcaaaaca atgggagcac aaaaagcatc aagaagatga
     2881 aacacaaagc caagaaagaa gagaacaatt tctctaatga ttcatctaaa gtgacgaagg
     2941 aattggagaa aacggattat atttaacaat tgttgttgtt aacttgttta ttgcagctta
     3001 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3061 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     3121 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3181 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3241 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3301 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3361 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3421 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3481 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3541 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3661 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     3721 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3781 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3901 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3961 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4021 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     4081 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4141 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4201 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4261 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4321 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4381 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4441 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4501 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4561 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     4621 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4681 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4741 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4801 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4861 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     4921 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     4981 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5041 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5101 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     5161 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5221 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5281 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     5341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6181 taccgccatg cat
//
