LOCUS       sequence-198865-        5352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and
            dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded 
            tetM resistance gene, origin of transfer of RP4 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Matteau D, Pepin ME, Baby V, Gauthier S, Arango Giraldo M, Knight 
            TF, Rodrigue S
  TITLE     Development of oriC-Based Plasmids for Mesoplasma florum.
  JOURNAL   Appl Environ Microbiol. 2017 Mar 17;83(7). pii: e03374-16. doi: 
            10.1128/AEM.03374-16. Print 2017 Apr 1.
  PUBMED    28115382
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2017-03-17"; volume: "83(7). pii: 
            e03374-16. doi"; pages: " 10.1128/AEM.03374-16. Print 2017 Apr 1"
COMMENT     Sequence Label: pMflT-o4
FEATURES             Location/Qualifiers
     source          1..5352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2325..2345
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     2346..2365
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2346..2364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2372..2389
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     rep_origin      complement(2472..3060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2552..2571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3066..3084
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     oriT            3142..3251
                     /note="incP origin of transfer"
ORIGIN
        1 tccctttagt gagggttaat gtcgtgactg ggaaaaccct ggcggaattc ggccagtcta
       61 catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat
      121 tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac
      181 tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt
      241 tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta
      301 tgctttgtat acctatggtt atgcataaaa atcccggtga taaaagtatt tatcactggg
      361 atttttatgc ccttttgggt ttttgaatgg aggaaatcta gatgaaaatc ataaatatcg
      421 gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt ttatataact
      481 ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact gataatacat
      541 tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt caatgggaaa
      601 atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca gaagtatacc
      661 gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat ggagttcaag
      721 ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca attttcttta
      781 taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat atcaaagaaa
      841 aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac gtttgcgtaa
      901 caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat gatttattag
      961 aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa gaaagtattc
     1021 gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt aacataggta
     1081 ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat cgtggtcctt
     1141 ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa cgtttagctt
     1201 atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt agtgaaaaag
     1261 aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc aaaattgacc
     1321 gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt aatagtgtac
     1381 ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac ccattacttc
     1441 aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat gctcttttag
     1501 aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact catgaaatta
     1561 ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta caagaaaaat
     1621 atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt ccattaaaaa
     1681 atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct tctattggtc
     1741 tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc gtaagtttag
     1801 gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac ggttgcgaac
     1861 aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat ggactttact
     1921 attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt ttagaacaag
     1981 ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa atctatgcac
     2041 cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct aatattgttg
     2101 atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca cgttgtattc
     2161 aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc ttaactgaat
     2221 taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt cctaatagtc
     2281 gtattgataa agttcgttat atgttcaaca aaatcacata ataatactag tagcggccgc
     2341 tgcagtaata cgactcacta tagggcatac gatttaggtg acactatagt agggataaca
     2401 gggtaattta gtaggtaggg ctatatggtc gttatgcgat actgatgcat aaaggccgcg
     2461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2641 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2881 aagttgtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3061 cgcgcaatta accctcacta aagggaacaa aagctggagc gctgcaggaa ttcgatatca
     3121 agcttatcga taccgtcgac gccggccagc ctcgcagagc aggattcccg ttgagcaccg
     3181 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
     3241 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc
     3301 aaaatcctgt atatcgtgcg aaaaagcatg gatataccga aaaaatcgct ataatgaccc
     3361 cgaagcaggg ttatgcagcg gaaaagcgct ggtacccagc gatgaggatg aacccactca
     3421 gggacctgat gtgacgtgcc gtgaacacga gcgtgtttag tttttgatgg ttgtcaagtt
     3481 cttttcattg tattcaaacc cccttttaaa gttgataatt cttgttaatt ataatataag
     3541 tgctggaaaa agtatagttt tttattgtta tttttaagaa aatttatcca ttgtgttttt
     3601 atatgaactt tttcaacatt ttttataacc ttttgtggaa aagcggaaaa aagatatata
     3661 aatactaaac tattgctttt tagtgttatt cattatgttt atttcttttt aaaatatttt
     3721 tttatccaca cattgtggaa gacttttatt attatgtgtt ttaatttatt ttatgaaggg
     3781 gaattaatat ggaaacaaaa gcgctttgag aaaaattaat taataagtta aagaaggaaa
     3841 aattaataga ccaagacatt attgaagaac atattgttac ttcagaatta attaaaattt
     3901 caaatacaga gttcgtaatc cttgttagaa gtaatcttgg tgtaactatt ttaaatgaat
     3961 ttaaagaagt atttgtgtac gaatttaaat aagttttaaa cagttatgtg tctgttgatt
     4021 ttttaactaa agaaattttt gagaaaaata caaaaaaaga gaataaaaaa gaacccataa
     4081 atactgtttt atctgaaaat gcacttactt ttgagaattt tatagttggg tcaagtaata
     4141 aacaagctaa tttagctgct aaaaatgttg ttgcaaaccc tggtatgagt tttaatcctt
     4201 tatttattta tggagattca ggactgggta aaacacattt attacaagct attaaaaatc
     4261 aagcagaatt aaatggaaaa aaagttttat atttaacatc tgaagaattt acaaaaagaa
     4321 ttgttaatgc tttaaacaaa ggtgatttaa gtgaaataga agaattaaaa actgagataa
     4381 attctaatga attctttatt ttagatgatg ttcaattttt aagtaaaaaa gataaaacaa
     4441 acgagttttt ctttaacatt attaacaatt ttactgaaaa tggtaaacaa ttagtttttt
     4501 ctagtgataa gactcctgaa ttgttaaatg gttttgataa aagaatgata actagattta
     4561 attcaggttt atcaacacct attaatgctt tagatattcc aactgccaaa ttgataattg
     4621 aagctgaaat taaaaaacaa ggtttaaaac aaaaaattaa agaagatgcc gttgtttact
     4681 tagctcaaaa ctttagcgat gatgttagaa aaattaaagg tttagttaat agattacttt
     4741 tctttggtat tcaaaacgat ttaggtcaca taattgattt ggaagatgtt attgacttat
     4801 ttaaagatac accttcagct aatttaggat tattaaatgt taaaaaaatt aaagaagttg
     4861 ttgctaaaaa atatgatgtt actataaaag ccatcgatgg taaagcaaga acaactgcta
     4921 taaaaaatgc tagacatctt tctatgtatt ttgcaaaaat aattttaaac catacatcaa
     4981 ctcaaattgg tgcagaattt ggtggaagag accatagtac agttttaagt gctatttcgc
     5041 gtattgaaaa attaatatat aaagaaaaag aattcaaaaa aatagttgag tctttaaaaa
     5101 acgaaataat aggaaaatag atgtaaattc gcatctattt tttttgtgtg gaaaagaaat
     5161 taaacttgtt gaataaaaag tcatttaaac ctttatttac cgttgtttta tgagtgtttt
     5221 ccacatatca actgcattat aaatattact tatataaaga ataatataat atataaaagc
     5281 atgcgtattt cttttgtttt tattttataa atatgagata atatatattg taaataagag
     5341 gtgttatatg ag
//
