LOCUS       sequence-198868-        8476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human codon optimized NgAgo; pCDNA3 vector backbone, mammalian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   O'Geen H, Ren C, Coggins NB, Bates SL, Segal DJ
  TITLE     Unexpected binding behaviors of bacterial Argonautes in human cells 
            cast doubts on their use as targetable gene regulators.
  JOURNAL   PLoS One. 2018 Mar 27;13(3):e0193818. doi: 
            10.1371/journal.pone.0193818. eCollection 2018.
  PUBMED    29584750
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-03-27"; volume: "13(3):e0193818. doi"; pages: " 
            10.1371/journal.pone.0193818. eCollection 2018"
COMMENT     Sequence Label: pCDNA-hNgAgo
FEATURES             Location/Qualifiers
     source          1..8476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        208..411
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     362..382
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     408..432
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             529..549
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             568..633
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             718..3378
                     /codon_start=1
                     /product="Argonaute protein from Natronobacterium gregoryi 
                     (Gao et al., 2016)"
                     /label=NgAgo
                     /note="reportedly a DNA-guided endonuclease that does not 
                     require a PAM site, although this interpretation has been 
                     challenged (Cyranoski, 2016)"
                     /translation="MTVIDLDSTTTADELTSGHTYDISVTLTGVYDNTDEQHPRMSLAF
                     EQDNGERRYITLWKNTTPKDVFTYDYATGSTYIFTNIDYEVKDGYENLTATYQTTVENA
                     TAQEVGTTDEDETFAGGEPLDHHLDDALNETPDDAETESDSGHVMTSFASRDQLPEWTL
                     HTYTLTATDGAKTDTEYARRTLAYTVRQELYTDHDAAPVATDGLMLLTPEPLGETPLDL
                     DCGVRVEADETRTLDYTTAKDRLLARELVEEGLKRSLWDDYLVRGIDEVLSKEPVLTCD
                     EFDLHERYDLSVEVGHSGRAYLHINFRHRFVPKLTLADIDDDNIYPGLRVKTTYRPRRG
                     HIVWGLRDECATDSLNTLGNQSVVAYHRNNQTPINTDLLDAIEAADRRVVETRRQGHGD
                     DAVSFPQELLAVEPNTHQIKQFASDGFHQQARSKTRLSASRCSEKAQAFAERLDPVRLN
                     GSTVEFSSEFFTGNNEQQLRLLYENGESVLTFRDGARGAHPDETFSKGIVNPPESFEVA
                     VVLPEQQADTCKAQWDTMADLLNQAGAPPTRSETVQYDAFSSPESISLNVAGAIDPSEV
                     DAAFVVLPPDQEGFADLASPTETYDELKKALANMGIYSQMAYFDRFRDAKIFYTRNVAL
                     GLLAAAGGVAFTTEHAMPGDADMFIGIDVSRSYPEDGASGQINIAATATAVYKDGTILG
                     HSSTRPQLGEKLQSTDVRDIMKNAILGYQQVTGESPTHIVIHRDGFMNEDLDPATEFLN
                     EQGVEYDIVEIRKQPQTRLLAVSDVQYDTPVKSIAAINQNEPRATVATFGAPEYLATRD
                     GGGLPRPIQIERVAGETDIETLTRQVYLLSQSHIQVHNSTARLPITTAYADQASTHATK
                     GYLVQTGAFESNVGFL"
     CDS             3454..3501
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3520..3576
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(3640..3659)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3684..3732
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3934..4362
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4021..4040)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4231..4252
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4371..4391)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4376..4705
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4556..4691
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4618..4637
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4772..5566
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4826..4845)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5436..5455
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5742..5863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5779..5798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5833..5852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5912..5928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5912..5928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5925..5947)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5936..5952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5960..5966,5967..5984,5985..5990))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6005..6026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6143..6160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6314..6902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6394..6413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7073..7864,7865..7933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7696..7715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7934..8038)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8113..8132)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8304..8476,1..207)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
       61 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      121 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      181 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      241 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      301 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      361 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      481 gaccgatcca gcctccggac tctagaggat cgaacccttg ccaccatgcc caagaagaag
      541 aggaaggtgg gaggctccgg aggaagcgac tataaggacc acgacggaga ctacaaggat
      601 catgatattg attacaaaga cgatgacgat aagggtggcg ggtccggcgg tggatccggt
      661 accggaggga gtggtggaag cggcggttct ggtggctcag gcggcagcgg ccggccaatg
      721 acagtgatag atctggattc aacaacaacc gcagacgagc ttacgtctgg acatacttat
      781 gatatcagcg tcactcttac cggggtctac gataacactg acgagcagca cccccgcatg
      841 tcccttgctt tcgaacaaga caatggcgag cgacgctaca taaccctctg gaagaacaca
      901 actcccaagg acgtcttcac ttacgattat gccacaggca gtacgtatat tttcactaat
      961 attgactacg aggtcaagga cggttatgaa aaccttaccg ccacctacca aacaacggtt
     1021 gaaaatgcta cagctcaaga agtcggcacc acggatgaag acgaaacatt tgccgggggc
     1081 gaaccattgg atcaccacct ggatgatgca ctgaacgaga cccccgatga tgcggaaacg
     1141 gaaagtgatt caggccatgt aatgacatca ttcgcgtcac gcgatcagct gccggagtgg
     1201 acattgcaca catacactct cacggccacg gatggggcta aaacagatac ggaatacgcg
     1261 agaagaactc tggcttacac tgttcggcaa gaactttata ccgaccacga cgcagcacca
     1321 gtggccaccg acggcttgat gcttctcact cctgagcctc tgggagagac ccctcttgat
     1381 cttgattgtg gcgtgcgcgt tgaggctgac gagacaagga cgttggatta cacaaccgca
     1441 aaagatagac ttctcgctcg ggaacttgtg gaggaggggt tgaaacggtc tctttgggac
     1501 gattacctcg tgaggggcat agatgaagtc ctgtcaaagg agcccgtgtt gacttgtgat
     1561 gagtttgacc tgcacgagcg ctatgacctg tctgtcgagg ttggtcattc tggccgggct
     1621 tatcttcaca ttaacttccg ccaccgcttc gtaccgaagc tcacgcttgc agacatagat
     1681 gacgacaaca tttatccggg gttgcgggtg aaaacaacct acagacctcg ccgaggccat
     1741 atagtctggg gtctccgcga cgaatgtgcg accgactccc ttaatacttt gggtaatcag
     1801 tcagtagtag cttatcaccg caataaccag acaccaatca ataccgacct tctcgatgct
     1861 attgaagccg cagaccgcag agtcgtcgaa acgcgcaggc aaggacatgg ggatgacgcc
     1921 gtatcattcc cccaggagct tctcgcagta gaacctaata cacatcaaat aaagcagttc
     1981 gcctccgacg ggtttcatca gcaggctagg tctaagaccc gactcagtgc atcaagatgt
     2041 tcagaaaaag cacaagcatt tgcggagcgg cttgaccctg tgcgattgaa cgggtctacg
     2101 gtggaattct cttctgagtt cttcacgggg aacaacgagc aacagctccg gttgttgtac
     2161 gaaaacggcg aatccgtgtt gacctttcgg gatggtgcca ggggggccca tcccgacgaa
     2221 acatttagta agggaatagt caatccacca gagtcatttg aggtagcagt cgtgctgcct
     2281 gaacagcagg ctgacacatg taaagcccag tgggacacta tggcagacct cttgaatcag
     2341 gcgggcgctc ctcctactcg atcagagacg gttcaatatg atgcgttctc ttctcctgaa
     2401 agtatttctc tcaatgtagc cggtgcgata gacccgtcag aagtcgatgc agcgttcgtc
     2461 gttctgcccc ctgatcagga gggtttcgct gatcttgctt ctccaaccga aacctatgac
     2521 gaactgaaaa aagcacttgc gaacatgggc atttattctc aaatggcata cttcgatcgc
     2581 tttcgggacg ctaagatttt ctacaccagg aacgttgcct tgggtttgct ggcagcagcg
     2641 ggcggcgtcg cttttacgac agaacacgcg atgcctggag atgcggacat gtttattgga
     2701 attgatgtga gccgaagcta tccggaagat ggggcgtcag gtcagattaa tatagcggct
     2761 accgcgacgg ccgtctataa ggacggtacg atattgggtc actcttccac gaggcctcaa
     2821 ctcggagaaa agctccaatc aactgacgtc agagatataa tgaagaatgc gatattgggc
     2881 tatcagcaag tgacgggtga atcccctacc catattgtta tccatcgcga cggatttatg
     2941 aacgaagatt tggatcccgc gactgagttt ctcaacgaac agggtgtgga gtatgacatt
     3001 gtggagatta ggaaacaacc ccaaacacgg ctcttggccg tgtcagacgt ccagtacgat
     3061 acgcctgtga agtcaattgc agccatcaat cagaacgaac cccgggcgac cgttgcgacc
     3121 tttggagctc cggagtatct tgcgactcgc gatggcggag ggttgccgag accgatacag
     3181 atagagaggg tcgccggcga aacggatata gaaacgctca cacgccaagt ataccttctt
     3241 tctcaatctc atatccaggt gcacaactca acggctcgct tgcccataac gacggcatac
     3301 gctgaccagg cgtcaacgca tgcgacgaag ggttatctgg tacaaacggg ggcgttcgag
     3361 agtaacgtcg gttttttggg tggtagtgga ggttcaggag gatccggggg gagcggaggg
     3421 agcgctagcg gtggaggctc cggaggtgga tctaaaaggc cggcggccac gaaaaaggcc
     3481 ggtcaggcaa aaaagaaaaa gggtggtagt ggaagcggag ctactaactt cagcctgctg
     3541 aagcaggctg gagacgtgga ggagaaccct ggacctgcgg ccgcatgaaa gggttcgatc
     3601 cctaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga aggagacaat
     3661 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     3721 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     3781 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     3841 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     3901 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     3961 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4021 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4081 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4141 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4201 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4261 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     4321 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4381 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4441 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4501 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4561 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4621 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     4681 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     4741 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     4801 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4861 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4921 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     4981 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5041 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5101 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5161 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5221 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5281 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     5341 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5401 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5461 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5521 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     5581 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5641 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5701 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5761 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5821 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5881 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5941 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6001 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6061 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6121 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6181 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6241 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6481 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     6541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6721 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6961 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7021 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7081 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7141 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7201 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7261 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7321 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7381 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7441 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7501 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7561 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7621 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7681 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7741 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7801 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7861 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7921 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7981 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8041 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     8101 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     8161 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     8221 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     8281 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     8341 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     8401 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     8461 tcccatagta acgcca
//
