LOCUS       sequence-19888-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pGP-FF
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(60..65,66..83,84..90)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    98..114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     103..125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             416..439
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(699..718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacggga attccgggct
       61 ttacacttta tgcttccggc tcgtataatg tgtcgacttg tgagcggata acaatttcac
      121 acaggaaaca gctatgcctc aacagcagca aatgcaacct cccaattcaa gtgcggacaa
      181 caaccctttg caacagcaat catcacaaaa taccgtacca aacgtcctca accaaattaa
      241 ccaaatcttt tctccagagg agcaacgcag cttattacaa gaagccatcg aaacctgcaa
      301 gaattttgaa aaaacacaat tgggtagtac gatgacggaa cctgtcaagc aaagttttat
      361 taggaaatac attgtgcaaa aggccctgag aaaaatccaa gctttggcgg ccgctgacta
      421 caaagacgat gatgacaaat ctagagtgca gtgataacct ctactcacct gcgtggatcc
      481 tctgcacagt agtaactaga gactagaaaa aggccgacaa gtcccgctcc gctgaagatc
      541 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      601 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      841 acgtagtggg ccatcgccct gatagacggg ttttcgccct ttgacgttgg agtccacgtt
      901 ctttaatagt ggactcttgt tccaaa
//
