LOCUS       sequence-198881-        4462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Delghandi MP, Johannessen M, Moens U
  TITLE     The cAMP signalling pathway activates CREB through PKA, p38 and MSK1
            in NIH 3T3 cells.
  JOURNAL   Cell Signal. 2005 Nov . 17(11):1343-51.
  PUBMED    16125054
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Signal. 2005 Nov . 17(11):1343-51."
COMMENT     Sequence Label: pGAL4-CREB S133A
FEATURES             Location/Qualifiers
     source          1..4462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..34
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     16..33
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(44..64)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             378..818
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     complement(1856..1872)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1856..1872)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    1987..2121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2024..2043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2078..2097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2131..2150)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2132..2150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2237..2254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2408..2996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2488..2507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3167..3958,3959..4027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3790..3809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4028..4132)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4200..4218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4256..4278)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4378..4397
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatcatacac atacgattta ggtgacacta tagaactcga tgtggaatgt gtgtcagtta
       61 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      121 tagtcagcaa ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      181 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      241 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      301 ggccgcctcg gcctctgagc tattccagaa gtagtgaaga ggcttttttg gaggagatct
      361 aagctgcctc ctgaaagatg aagctactgt cttctatcga acaagcatgc gatatttgcc
      421 gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca
      481 actgggagtg tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga
      541 cagaagtgga atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag
      601 aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag
      661 gattatttgt acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg
      721 agactgatat gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag
      781 agagtagtaa caaaggtcaa agacagttga ctgtatcgcc ggaattcgga tccgactctg
      841 gagcagacaa ccagcagagt ggagatgctg ctgtaacaga agctgaaagt caacaaatga
      901 cagttcaagc ccagccacag attgccacat tagcccaggt atccatgcca gcagctcatg
      961 cgacgtcatc tgctcccact gtaaccttag tgcagctgcc caatgggcag acagtccagg
     1021 tccatggggt catccaggcg gcccagccat cagttattca gtctccacaa gtccaaacag
     1081 ttcagtcttc ctgtaaggac ttaaaaagac ttttctccgg aactcagatt tcaactattg
     1141 cagaaagtga agattcacag gagtctgtgg atagtgtaac tgattcccaa aaacgaaggg
     1201 aaatcctttc aaggaggcct gcctacagga aaattttgaa tgacttatct tctgatgcac
     1261 caggggtgcc aaggattgaa gaagaaaaat cagaagaaga gacttcagcc cctgccatca
     1321 ccactgtaac agtgccaacc ccgatttacc aaactagcag tgggcagtat attgccatta
     1381 cccagggagg agcaatacag ctggctaaca atggtaccga tggggtacag ggcctgcaga
     1441 cattaaccat gaccaatgca gctgccactc agccgggtac taccattcta caatatgcac
     1501 agaccactga tggacagcag attctagtgc ccagcaacca agttgttgtt caagctgcct
     1561 ctggtgatgt acaaacatac cagattcgca cagcacccac tagcaccatt gcccctggag
     1621 ttgttatggc gtcctcccca gcacttccta cacagcctgc tgaagaagca gcacgaaaga
     1681 gagaggttcg tctaatgaag aacagggaag cagcaagaga atgtcgtaga aagaagaaag
     1741 aatatgtgaa atgtttagag aacagagtgg cagtgcttga aaaccaaaac aaaacattga
     1801 ttgaggagct aaaagcactt aaggaccttt actgccacaa gtcagattaa tttgggatcc
     1861 actagttcta gataagtaat gatcataatc agccatatca catctgtaga ggttttactt
     1921 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1981 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2041 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2101 gtatcttatc atgtctggat ctgccggtct ccctatagtg agtcgtatta atttcgataa
     2161 gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2821 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4141 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4201 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4261 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     4321 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     4381 catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta gaacgcggct
     4441 acaattaata cataacctta tg
//
