LOCUS       sequence-198882-        9278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian
            cells driven by UBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Gu X, Orozco JM, Saxton RA, Condon KJ, Liu GY, Krawczyk PA, Scaria 
            SM, Harper JW, Gygi SP, Sabatini DM
  TITLE     SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2017 Nov 10;358(6364):813-818. doi: 
            10.1126/science.aao3265.
  PUBMED    29123071
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-10- 10"; volume: "358"; 
            issue: "6364"; pages: "813-818"
COMMENT     Sequence Label: pLJC5-Flag-SAMTOR
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    39..60
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(75..80,81..98,99..105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    113..129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     118..140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     137..153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     137..153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     172..192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        174..192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        220..446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             447..627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    674..799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1292..1525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1710..1754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2043..3254
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3091..3110
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3275..3284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3284..3307
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4591..4708
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4757..5267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5186..5204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5289..5888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5289..5308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5785..5805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6016..6249
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6321..6442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6358..6377)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6412..6431
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6482..6617
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6637..6656)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6638..6656)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6666..6683)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6666..6682)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6675..6697)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6824..7279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6911..6930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7121..7142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7305..7409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7410..7478,7479..8270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7628..7647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8441..9029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8930..8949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9183..9200
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
       61 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      121 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      181 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      241 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      301 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      361 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      421 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      481 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      541 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      601 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      661 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      721 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      781 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      841 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
      901 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
      961 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1021 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1081 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcagc
     1141 agctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1201 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1261 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1321 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1381 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1441 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1501 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1561 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1621 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1681 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1741 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1801 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1861 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     1921 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     1981 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcgggat
     2041 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     2101 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     2161 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     2221 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     2281 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     2341 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     2401 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     2461 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     2521 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     2581 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     2641 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     2701 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     2761 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     2821 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     2881 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     2941 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     3001 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     3061 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     3121 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     3181 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     3241 cttttttgtt agacgaagcg ctagcgctac cggtgccacc atggactaca aggatgacga
     3301 tgacaagggt ggtggtggtg gtgcgtcgac gatggagcca ggggccggcg gccgaaatac
     3361 tgctcgtgcg cagagggccg ggtccccgaa cactccgccg ccccgggagc aggagcggaa
     3421 actggagcag gagaagctct ccggtgtggt gaagagcgtc caccggcggc tccgcaagaa
     3481 gtaccgagaa gtgggagatt ttgataagat ctggcgagaa cactgtgagg atgaagaaac
     3541 tctttgtgaa tatgccgttg caatgaaaaa tttggcagat aatcattggg caaaaacttg
     3601 tgagggcgaa ggtcgtattg aatggtgttg tagtgtatgc agagaatatt tccaaaatgg
     3661 tgggaagaga aaagcacttg agaaagatga aaaaagagct gtacttgcca ctaagaccac
     3721 tccagcctta aatatgcatg agtcttctca acttgaaggt catttaacca acctcagctt
     3781 tacaaaccct gaatttataa ctgagttgct acaagcctca ggaaaaatca gattacttga
     3841 tgttggcagc tgctttaacc catttctgaa gtttgaagaa tttctaactg ttggcataga
     3901 tattgtacct gctgtagaga gtgtctataa atgtgatttc ctgaacttac agcttcagca
     3961 accactccag cttgcacagg atgctataga tgcttttttg aagcagctga aaaaccctat
     4021 tgattctctt cctggagagc ttttccatgt ggttgttttc tctctccttc tttcttattt
     4081 tccatctcct taccagcgat ggatttgctg caagaaagcc catgaactgt tagtgttaaa
     4141 tggtttatta ctaatcatca cacctgattc ctcccatcag aaccgtcatg ctatgatgat
     4201 gaaaagctgg aagattgcta tagagtccct gggctttaaa cgcttcaagt actcaaaatt
     4261 ttcacatatg catctgatgg catttaggaa aatctctcta aaaaccacaa gtgacttggt
     4321 tagtaggaac tacccaggaa tgttatatat tcctcaagat ttcaacagta tagaagatga
     4381 ggaatattct aacccttcct gctatgttcg atcagatata gaagatgaac aactagcata
     4441 tggtttcaca gaactccctg atgcgccata tgactcagat tctggagaaa gtcaagccag
     4501 ctctattcct ttctatgagc tagaagaccc catattactt ttaagttaag aattctcgac
     4561 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     4621 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     4681 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     4741 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     4801 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     4861 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     4921 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     4981 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     5041 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     5101 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     5161 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     5221 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg
     5281 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5341 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5401 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5461 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5521 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5581 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5641 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5701 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5761 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5821 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5881 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     5941 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6001 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6061 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6121 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6181 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6241 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6301 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6361 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6421 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6481 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6541 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6601 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6661 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6721 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6781 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6961 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7021 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7201 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7261 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7321 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7381 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7441 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7501 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7561 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7621 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7681 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7741 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7801 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7861 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7921 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7981 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8041 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8101 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8161 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8221 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8281 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8341 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8581 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8761 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8821 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8881 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8941 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9001 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9061 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9181 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9241 cgcgttggcc gattcattaa tgcagctggc acgacagg
//
