LOCUS       sequence-198890-        4956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF219 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF219
FEATURES             Location/Qualifiers
     source          1..4956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2299..2398)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2415..2434)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2416..2434)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2439..2455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2439..2455)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2886..3677
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3770..4358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4259..4278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4512..4529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4688..4715)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4807..4893)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4942..4956,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4956,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagttgg catggagggc tcacgtcccc gcgccccgag cggccactta gcgccgtcgc
      181 cgccggcttt cgacggcgag ctggatctgc agcgatactc caacgggcca gccgtgagcg
      241 cagggtcgct cgggatggga gcggtgagct ggtctgagag tcgtgcaggc gaacggcgct
      301 tcccctgccc tgtatgcggg aagcgcttcc gcttcaactc tatccttgct ttgcacctgc
      361 gggcgcaccc aggagcccag gccttccagt gccctcactg cggccaccgc gcggcgcagc
      421 gggctctgct gcgctcgcac ctgcgcacac accagcccga gcgcccacgt agtcctgctg
      481 cacgcctgtt gctggagttg gaagagcgcg cgctactacg cgaggcccga ctggggagag
      541 cccgaagctc agggggcatg caggccaccc ctgccactga gggtctggcg cggccccagg
      601 ctccttcatc gtccgccttc cgttgcccct actgcaaagg caagtttcgc acctcggcgg
      661 agcgcgaacg ccacctgcac atcctgcata ggccctggaa gtgcggcctg tgcagtttcg
      721 gctccagcca ggaggaggag ctgctgcacc acagcctgac ggcccacggg gctcccgagc
      781 gtcccctggc ggccacctcc gctgcgcctc cgcctcagcc tcagcctcag cctccacccc
      841 agcccgaacc cagatcagtc ccccagccgg agccggagcc ggagcccgaa cgtgaggcaa
      901 ccccgaccac agctcctgcc gctcccgagg agcccccagc gcctccggag ttccgctgcc
      961 aagtgtgcgg ccagagcttt acacagtctt ggtttctcaa gggccacatg cgtaagcaca
     1021 aggcctcctt cgatcatgcg tgtccggtgt gcggccgctg cttcaaggag ccctggttcc
     1081 ttaagaacca catgaaggtg cacgccagca agctgggccc actgcgtgcc ccggggcctg
     1141 cctccgggcc tgcccgcgcc ccccagcctc ctgacctcgg cctgctggcc tatgagccgt
     1201 tgggcccagc gctcctcttg gccccggctc ccaccccggc cgagcgccgt gagcccccga
     1261 gcctcttggg ctacctgagc ctgcgagctg gcgagggccg gcccaacggc gagggtgctg
     1321 agcccggtcc cggccgcagc ttcggaggct tccgcccgct gtcctctgct ctcccggccc
     1381 gggctcgccg gcaccgtgcg gaggagcctg aggaagaaga ggaggtggtg gaggccgagg
     1441 aggaaacctg ggcccggggc aggtcgctgg gctctctggc ttccctgcac ccgcgcccgg
     1501 gtgagggacc ggggcactct gcctctgctg ctggggccca ggcaagatcg accgccacgc
     1561 aggaagagaa tgggctgttg gttggaggga cccggcctga agggggccgg ggcgccaccg
     1621 gcaaggattg tcctttctgc ggaaaatctt tccgctcagc acatcacctc aaagtgcatc
     1681 tgcgagtgca cacaggcgag cggccctaca agtgtccgca ctgcgactac gcgggcaccc
     1741 agtccggctc gctcaagtat cacctacagc gccaccaccg ggagcagagg agcggggccg
     1801 gccccgggcc acccccggag ccaccgcctc cttcccagcg gggttcggcc ccgcaatctg
     1861 gagccaagcc gtctccgcag cctgcgacct gggtggaggg cgcctcaagt ccccggcctc
     1921 cttctagcgg tgctgggccg gggtcccgtc ggaagcccgc cagccctggg aggaccctgc
     1981 gcaacgggcg aggcggtgag gccgaacccc tggacctgtc cttgcgggca gggccgggag
     2041 gcgaggccgg gcctgggggt gccctccacc gctgcctctt ctgcccgttc gccactggag
     2101 ccccagagct catggccttg caccttcaag tgcaccacag ccgccgggct aggggccgcc
     2161 ggccacccca ggctgacgcg tccccgccct atgcccgagt accatcagga gagacccctc
     2221 ccagtccttc gcaggaaggg gaggagggct ccgggctgtc cagacccgga gaggcagggc
     2281 tgggggggca agaacggtac ccaactttct tgtacaaagt tggcattata agaaagcatt
     2341 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2401 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2461 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2521 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2581 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2641 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2701 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2761 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2821 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2881 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2941 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3001 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     3061 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3121 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3181 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3241 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3301 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3361 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3421 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3481 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3541 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3601 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3661 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3721 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3781 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3841 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3901 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3961 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4021 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4081 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4141 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4201 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4261 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4321 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4381 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4441 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4501 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4561 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4621 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4681 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4741 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4801 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4861 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     4921 taacgctagc atggatgttt tcccagtcac gacgtt
//
