LOCUS       sequence-198939-        9736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag GRK5
FEATURES             Location/Qualifiers
     source          1..9736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    805..1004
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1205..1621
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1551..1573
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1589..1605
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..1686
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1741..1765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3536..3560)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3619..4080
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3675..3692)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3902..3921
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4085..4879
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4139..4158)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4749..4768
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5066..5535
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5571..5675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5676..5744,5745..6536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5894..5913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8302..8890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8791..8810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9044..9061
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9165..9592)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9275..9296)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9487..9506
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgcg
      601 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
      661 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
      721 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
      781 accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg
      841 tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg
      901 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
      961 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     1021 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     1081 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     1141 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1201 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1261 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1321 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1381 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1441 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1501 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1561 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1621 cggccggatc cagaattcgc caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
     1681 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
     1741 acaagtttgt acaaaaaagt tggcatggag ctggaaaaca tcgtggccaa cacggtcttg
     1801 ctgaaagcca gggaaggggg cggaggaaag cgcaaaggga aaagcaagaa gtggaaagaa
     1861 atcctgaagt tccctcacat tagccagtgt gaagacctcc gaaggaccat agacagagat
     1921 tactgcagtt tatgtgacaa gcagccaatc gggaggctgc ttttccggca gttttgtgaa
     1981 accaggcctg ggctggagtg ttacattcag ttcctggact ccgtggcaga atatgaagtt
     2041 actccagatg aaaaactggg agagaaaggg aaggaaatta tgaccaagta cctcacccca
     2101 aagtcccctg ttttcatagc ccaagttggc caagacctgg tctcccagac ggaggagaag
     2161 ctcctacaga agccgtgcaa agaactcttt tctgcctgtg cacagtctgt ccacgagtac
     2221 ctgaggggag aaccattcca cgaatatctg gacagcatgt tttttgaccg ctttctccag
     2281 tggaagtggt tggaaaggca accggtgacc aaaaacactt tcaggcagta tcgagtgcta
     2341 ggaaaagggg gcttcgggga ggtctgtgcc tgccaggttc gggccacggg taaaatgtat
     2401 gcctgcaagc gcttggagaa gaagaggatc aaaaagagga aaggggagtc catggccctc
     2461 aatgagaagc agatcctcga gaaggtcaac agtcagtttg tggtcaacct ggcctatgcc
     2521 tacgagacca aggatgcact gtgcttggtc ctgaccatca tgaatggggg tgacctgaag
     2581 ttccacatct acaacatggg caaccctggc ttcgaggagg agcgggcctt gttttatgcg
     2641 gcagagatcc tctgcggctt agaagacctc caccgtgaga acaccgtcta ccgagatctg
     2701 aaacctgaaa acatcctgtt agatgattat ggccacatta ggatctcaga cctgggcttg
     2761 gctgtgaaga tccccgaggg agacctgatc cgcggccggg tgggcactgt tggctacatg
     2821 gctccagagg tcctgaacaa ccagaggtac ggcctgagcc ccgactactg gggccttggc
     2881 tgcctcatct atgagatgat cgagggccag tcgccgttcc gcggccgcaa ggagaaggtg
     2941 aagcgggagg aggtggaccg ccgggtcctg gagacggagg aggtgtactc ccacaagttc
     3001 tccgaggagg ccaagtccat ctgcaagatg ctgctcacga aagatgcgaa gcagaggctg
     3061 ggctgccagg aggagggggc tgcagaggtc aagagacacc ccttcttcag gaacatgaac
     3121 ttcaagcgct tagaagccgg gatgttggac cctcccttcg ttccagaccc ccgcgctgtg
     3181 tactgtaagg acgtgctgga catcgagcag ttctccactg tgaagggcgt caatctggac
     3241 cacacagacg acgacttcta ctccaagttc tccacgggct ctgtgtccat cccatggcaa
     3301 aacgagatga tagaaacaga atgctttaag gagctgaacg tgtttggacc taatggtacc
     3361 ctcccgccag atctgaacag aaaccaccct ccggaaccgc ccaagaaagg gctgctccag
     3421 agactcttca agcggcagca tcagaacaat tccaagagtt cgcccagctc caagaccagt
     3481 tttaaccacc acataaactc aaaccatgtc agctcgaact ccaccggaag cagcttgcca
     3541 actttcttgt acaaagtggt ttgatctagt cgaccaattc cggttatttt ccaccatatt
     3601 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     3661 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     3721 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     3781 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     3841 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     3901 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     3961 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4021 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     4081 taccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4141 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4201 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4261 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4321 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4381 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4441 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4501 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4561 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     4621 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4681 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4741 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4801 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4861 tcttgacgag ttcttctgag cgggactctg gggttcgcat cgataaaata aaagatttta
     4921 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
     4981 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
     5041 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     5101 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
     5161 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     5221 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     5281 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     5341 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     5401 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     5461 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     5521 agcgggggtc tttcacaggc cagtggggcc gcacttttcg gggaaatgtg cgcggaaccc
     5581 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5641 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5701 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5761 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5821 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5881 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5941 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6001 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6061 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6121 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6181 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6241 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6301 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6361 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6421 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6481 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6541 cagaccaagt ttactcatat atactttaga ttgatttgcg gccgcgaaaa ggacaagcag
     6601 cgaaaattca cgcccccttg ggaggtggcg gcatatgcaa aggatagcac tcccactcta
     6661 ctactgggta tcatatgctg actgtatatg catgaggata gcatatgcta cccggataca
     6721 gattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     6781 gattaggata gcctatgcta cccagatata aattaggata gcatatacta cccagatata
     6841 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     6901 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca tggcaacatt
     6961 agcccaccgt gctctcagcg acctcgtgaa tatgaggacc aacaaccctg tgcttggcgc
     7021 tcaggcgcaa gtgtgtgtaa tttgtcctcc agatcgcagc aatcgcgccc ctatcttggc
     7081 ccgcccacct acttatgcag gtattccccg gggtgccatt agtggttttg tgggcaagtg
     7141 gtttgaccgc agtggttagc ggggttacaa tcagccaagt tattacaccc ttattttaca
     7201 gtccaaaacc gcagggcggc gtgtgggggc tgacgcgtgc ccccactcca caatttcaaa
     7261 aaaaagagtg gccacttgtc tttgtttatg ggccccattg gcgtggagcc ccgtttaatt
     7321 ttcgggggtg ttagagacaa ccagtggagt ccgctgctgt cggcgtccac tctctttccc
     7381 cttgttacaa atagagtgta acaacatggt tcacctgtct tggtccctgc ctgggacaca
     7441 tcttaataac cccagtatca tattgcacta ggattatgtg ttgcccatag ccataaattc
     7501 gtgtgagatg gacatccagt ctttacggct tgtccccacc ccatggattt ctattgttaa
     7561 agatattcag aatgtttcat tcctacacta gtatttattg cccaaggggt ttgtgagggt
     7621 tatattggtg tcatagcaca atgccaccac tgaacccccc gtccaaattt tattctgggg
     7681 gcgtcacctg aaaccttgtt ttcgagcacc tcacatacac cttactgttc acaactcagc
     7741 agttattcta ttagctaaac gaaggagaat gaagaagcag gcgaagattc aggagagttc
     7801 actgcccgct ccttgatctt cagccactgc ccttgtgact aaaatggttc actaccctcg
     7861 tggaatcctg accccatgta aataaaaccg tgacagctca tggggtggga gatatcgctg
     7921 ttccttagga cccttttact aaccctaatt cgatagcata tgcttcccgt tgggtaacat
     7981 atgctattga attagggtta gtctggatag tatatactac tacccgggaa gcatatgcta
     8041 cccgtttagg gttaacaagg gggccttata aacactattg ctaatgccct cttgagggtc
     8101 cgcttatcgg tagctacaca ggcccctctg attgacgttg gtgtagcctc ccgtagtctt
     8161 cctgggcccc tgggaggtac atgtcggccg caaaaggatc taggtgaaga tcctttttga
     8221 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8281 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8341 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8401 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8461 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8521 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8581 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8641 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8701 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8761 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8821 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8881 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8941 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9001 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9061 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9121 atgcaactat ggttggcgcg ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     9181 ttgttaaatc agctcatttt ttaaccaata gaccgaaatc ggcaaaatcc cttataaatc
     9241 aaaagaatag cccgagatag agttgagtgt tgttccagtt tggaacaaga gtccactatt
     9301 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     9361 acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     9421 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     9481 aaaggaaggg aagaaagcga aaggagcggg cgctaaggcg ctggcaagtg tagcggtcac
     9541 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgccgatctt
     9601 caatattggc cattagccat attattcatt ggttatatag cataaatcaa tattggctat
     9661 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     9721 atatgaccgc catgtt
//
