LOCUS       sequence-198956-       10603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of
            serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC 
            phosphorylation of the tail. Also carries S1915A mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Rai V, Thomas DG, Beach JR, Egelhoff TT
  TITLE     Myosin IIA Heavy Chain Phosphorylation Mediates Adhesion Maturation 
            and Protrusion in Three Dimensions.
  JOURNAL   J Biol Chem. 2017 Feb 24;292(8):3099-3111. doi: 
            10.1074/jbc.M116.733402. Epub 2017 Jan 4.
  PUBMED    28053086
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.733402"; journalName: "J Biol Chem"; date: 
            "2017-02-24- 24"; volume: "292"; issue: "8"; pages: "3099-3111"
COMMENT     Sequence Label: pEGFP-NMHC-IIA-S1916A
FEATURES             Location/Qualifiers
     source          1..10603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        101..404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        405..608
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     559..579
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      647..656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(653..655,656..658,659..1369)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(653..655,656..658,659..1369)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(698..719)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(959..978)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1306..1327
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7431..7552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7468..7487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7522..7541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7559..8014)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7696..7717)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7908..7927
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8041..8145
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8147..8504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8147..8338
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8170..8190)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8355..8490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8539..9333
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8539..9333
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8593..8612)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9203..9222
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9521..9540)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9565..9612
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9941..10529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10430..10449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
       61 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      121 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      181 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      241 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      301 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      361 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      421 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      481 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      541 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      601 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag
      661 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      721 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      781 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      841 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      901 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      961 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1021 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1081 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1141 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1201 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1261 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1321 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt actcagatct
     1381 cgagctcaag cttatggcac agcaagctgc cgataagtat ctctatgtgg ataaaaactt
     1441 catcaacaat ccgctggccc aggccgactg ggctgccaag aagctggtat gggtgccttc
     1501 cgacaagagt ggctttgagc cagccagcct caaggaggag gtgggcgaag aggccatcgt
     1561 ggagctggtg gagaatggga agaaggtgaa ggtgaacaag gatgacatcc agaagatgaa
     1621 cccgcccaag ttctccaagg tggaggacat ggcagagctc acgtgcctca acgaagcctc
     1681 ggtgctgcac aacctcaagg agcgttacta ctcagggctc atctacacct attcaggcct
     1741 gttctgtgtg gtcatcaatc cttacaagaa cctgcccatc tactctgaag agattgtgga
     1801 aatgtacaag ggcaagaaga ggcacgagat gccccctcac atctatgcca tcacagacac
     1861 cgcctacagg agtatgatgc aagaccgaga agatcaatcc atcttgtgca ctggtgaatc
     1921 tggagctggc aagacggaga acaccaagaa ggtcatccag tatctggcgt acgtggcgtc
     1981 ctcgcacaag agcaagaagg accagggcga gctggagcgg cagctgctgc aggccaaccc
     2041 catcctggag gccttcggga acgccaagac cgtgaagaat gacaactcct cccgcttcgg
     2101 caaattcatt cgcatcaact ttgatgtcaa tggctacatt gttggagcca acattgagac
     2161 ttatcttttg gagaaatctc gtgctatccg ccaagccaag gaagaacgga ccttccacat
     2221 cttctattat ctcctgtctg gggctggaga gcacctgaag accgatctcc tgttggagcc
     2281 gtacaacaaa taccgcttcc tgtccaatgg acacgtcacc atccccgggc agcaggacaa
     2341 ggacatgttc caggagacca tggaggccat gaggattatg ggcatcccag aagaggagca
     2401 aatgggcctg ctgcgggtca tctcaggggt tcttcagctc ggcaacatcg tcttcaagaa
     2461 ggagcggaac actgaccagg cgtccatgcc cgacaacaca gctgcccaaa aggtgtccca
     2521 tctcttgggt atcaatgtga ccgatttcac cagaggaatc ctcaccccgc gcatcaaggt
     2581 gggacgggat tacgtccaga aggcgcagac taaagagcag gctgactttg ccatcgaggc
     2641 cttggccaag gcgacctatg agcggatgtt ccgctggctg gtgctgcgca tcaacaaggc
     2701 tctggacaag accaagaggc agggcgcctc cttcatcggg atcctggaca ttgccggctt
     2761 cgagatcttt gatctgaact cgtttgagca gctgtgcatc aattacacca atgagaagct
     2821 gcagcagctc ttcaaccaca ccatgttcat cctggagcag gaggagtacc agcgcgaggg
     2881 catcgagtgg aacttcatcg actttggcct cgacctgcag ccctgcatcg acctcattga
     2941 gaagccagca ggccccccgg gcattctggc cctgctggac gaggagtgct ggttccccaa
     3001 agccaccgac aagagcttcg tggagaaggt gatgcaggag cagggcaccc accccaagtt
     3061 ccagaagccc aagcagctga aggacaaagc tgatttctgc attatccact atgccggcaa
     3121 ggtggattac aaagctgacg agtggctgat gaagaacatg gatcccctga atgacaacat
     3181 cgccacactg ctccaccagt cctctgacaa gtttgtctcg gagctgtgga aggatgtgga
     3241 ccgcatcatc ggcctggacc aggtggccgg catgtcggag accgcactgc ccggggcctt
     3301 caagacgcgg aagggcatgt tccgcactgt ggggcagctt tacaaggagc agctggccaa
     3361 gctgatggct acgctgagga acacgaaccc caactttgtc cgctgcatca tccccaacca
     3421 cgagaagaag gccggcaagc tggacccgca tctcgtgctg gaccagctgc gctgcaacgg
     3481 tgttctcgag ggcatccgta tctgccgcca gggcttcccc aacagggtgg tcttccagga
     3541 gtttcggcag agatatgaga tcctgactcc aaactccatt cccaagggtt tcatggacgg
     3601 gaagcaggcg tgcgtgctca tgataaaagc cctggagctc gacagcaatc tgtaccgcat
     3661 tggccagagc aaagtcttct tccgtgccgg tgtgctggcc cacctggagg aggagcgaga
     3721 cctgaagatc accgacgtca tcatagggtt ccaggcctgc tgcaggggct acctggccag
     3781 gaaagcattt gccaagcggc agcagcagct taccgccatg aaggtcctcc agcggaactg
     3841 cgctgcctac ctgaagctgc ggaactggca gtggtggcgg ctcttcacca aggtcaagcc
     3901 gctgctgcag gtgagccggc aggaggagga gatgatggcc aaggaggagg agctggtgaa
     3961 ggtcagagag aagcagctgg ctgcggagaa caggctcacg gagatggaga cgctgcagtc
     4021 tcagctcatg gcagagaaat tgcagctgca ggagcagctc caggcagaaa ccgagctgtg
     4081 tgccgaggct gaggagctcc gggcccgcct gaccgccaag aagcaggaat tagaagagat
     4141 ctgccatgac ctagaggcca gggtggagga ggaggaggag cgctgccagc acctgcaggc
     4201 ggagaagaag aagatgcagc agaacatcca ggagcttgag gagcagctgg aggaggagga
     4261 gagcgcccgg cagaagctgc agctggagaa ggtgaccacc gaggcgaagc tgaaaaagct
     4321 ggaggaggag cagatcatcc tggaggacca gaactgcaag ctggccaagg aaaagaaact
     4381 gctggaagac agaatagctg agttcaccac caacctcaca gaagaggagg agaaatctaa
     4441 gagcctcgcc aagctcaaga acaagcatga ggcaatgatc actgacttgg aagagcgcct
     4501 ccgcagggag gagaagcagc gacaggagct ggagaagacc cgccggaagc tggagggaga
     4561 ctccacagac ctcagcgacc agatcgccga gctccaggcc cagatcgcgg agctcaagat
     4621 gcagctggcc aagaaagagg aggagctcca ggccgccctg gccagagtgg aagaggaagc
     4681 tgcccagaag aacatggccc tcaagaagat ccgggagctg gaatctcaga tctctgaact
     4741 ccaggaagac ctggagtctg agcgtgcttc caggaataaa gctgagaagc agaaacggga
     4801 ccttggggaa gagctagagg cgctgaaaac agagttggag gacacgctgg attccacagc
     4861 tgcccagcag gagctcaggt caaaacgtga gcaggaggtg aacatcctga agaagaccct
     4921 ggaggaggag gccaagaccc acgaggccca gatccaggag atgaggcaga agcactcaca
     4981 ggccgtggag gagctggcgg agcagctgga gcagacgaag cgggtgaaag caaacctcga
     5041 gaaggcaaag cagactctgg agaacgagcg gggggagctg gccaacgagg tgaaggtgct
     5101 gctgcagggc aaaggggact cggagcacaa gcgcaagaaa gtggaggcgc agctgcagga
     5161 gctgcaggtc aagttcaacg agggagagcg cgtgcgcaca gagctggccg acaaggtcac
     5221 caagctgcag gtggagctgg acaacgtgac cgggcttctc agccagtccg acagcaagtc
     5281 cagcaagctc accaaggact tctccgcgct ggagtcccag ctgcaggaca ctcaggagct
     5341 gctgcaggag gagaaccggc agaagctgag cctgagcacc aagctcaagc aggtggagga
     5401 cgagaagaat tccttccggg agcagctgga ggaggaggag gaggccaagc acaacctgga
     5461 gaagcagatc gccaccctcc atgcccaggt ggccgacatg aaaaagaaga tggaggacag
     5521 tgtggggtgc ctggaaactg ctgaggaggt gaagaggaag ctccagaagg acctggaggg
     5581 cctgagccag cggcacgagg agaaggtggc cgcctacgac aagctggaga agaccaagac
     5641 gcggctgcag caggagctgg acgacctgct ggtggacctg gaccaccagc gccagagcgc
     5701 gtgcaacctg gagaagaagc agaagaagtt tgaccagctc ctggcggagg agaagaccat
     5761 ctctgccaag tatgcagagg agcgcgaccg ggctgaggcg gaggcccgag agaaggagac
     5821 caaggctctg tcgctggccc gggccctgga ggaagccatg gagcagaagg cggagctgga
     5881 gcggctcaac aagcagttcc gcacggagat ggaggacctt atgagctcca aggatgatgt
     5941 gggcaagagt gtccacgagc tggagaagtc caagcgggcc ctagagcagc aggtggagga
     6001 gatgaagacg cagctggaag agctggagga cgagctgcag gccaccgaag atgccaagct
     6061 gcggttggag gtcaacctgc aggccatgaa ggcccagttc gagcgggacc tgcagggccg
     6121 ggacgagcag agcgaggaga agaagaagca gctggtcaga caggtgcggg agatggaggc
     6181 agagctggag gacgagagga agcagcgctc gatggcagtg gccgcccgga agaagctgga
     6241 gatggacctg aaggacctgg aggcgcacat cgactcggcc aacaagaacc gggacgaagc
     6301 catcaaacag ctgcggaagc tgcaggccca gatgaaggac tgcatgcgcg agctggatga
     6361 cacccgcgcc tctcgtgagg agatcctggc ccaggccaaa gagaacgaga agaagctgaa
     6421 gagcatggag gccgagatga tccagttgca ggaggaactg gcagccgcgg agcgtgccaa
     6481 gcgccaggcc cagcaggagc gggatgagct ggctgacgag atcgccaaca gcagcggcaa
     6541 aggagccctg gcgttagagg agaagcggcg tctggaggcc cgcatcgccc agctggagga
     6601 ggagctggag gaggagcagg gcaacacgga gctgatcaac gaccggctga agaaggccaa
     6661 cctgcagatc gaccagatca acaccgacct gaacctggag cgcagccacg cccagaagaa
     6721 cgagaatgct cggcagcagc tggaacgcca gaacaaggag cttaaggtca agctgcagga
     6781 gatggagggc actgtcaagt ccaagtacaa ggcctccatc accgccctcg aggccaagat
     6841 tgcacagctg gaggagcagc tggacaacga gaccaaggag cgccaggcag cctgcaaaca
     6901 ggtgcgtcgg accgagaaga agctgaagga tgtgctgctg caggtggatg acgagcggag
     6961 gaacgccgag cagtacaagg accaggccga caaggcatct acccgcctga agcagctcaa
     7021 gcggcagctg gaggaggccg aagaggaggc ccagcgggcc aacgcctccc gccggaaact
     7081 gcagcgcgag ctggaggacg ccactgagac ggccgatgcc atgaaccgcg aagtcgccgc
     7141 cctaaagaac aagctcaggc gcggggacct gccgtttgtc gtgccccgcc gaatggcccg
     7201 gaaaggcgcc ggggatggct ccgacgaaga ggtagatggc aaagcggatg gggctgaggc
     7261 caaacctgcc gaataaacta gtcgacggta ccgcgggccc gggatccacc ggatctagat
     7321 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     7381 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     7441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     7561 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     7621 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     7681 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     7741 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     7801 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     7861 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     7921 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     7981 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     8041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     8161 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     8221 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     8281 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     8341 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8401 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8461 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     8521 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     8581 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     8641 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     8701 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     8761 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8821 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8881 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8941 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     9001 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     9061 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     9121 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     9181 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     9241 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     9301 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     9361 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     9421 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     9481 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     9541 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     9601 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     9661 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     9721 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     9781 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9841 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9901 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9961 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10021 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10081 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10141 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10201 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10261 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10321 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10381 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10441 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10501 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10561 tggccttttg ctggcctttt gctcacatgt tctttcctgc gtt
//
